Attenuation potentials of royal jelly against hydroxyurea-induced infertility through inhibiting oxidation and release of pro-inflammatory cytokines in male rats

Tohamy, Hossam G.; El-Karim, Dina R. Gad; El‐Sayed, Yasser S.

doi:10.1007/s11356-019-05521-3

Cited by 13 publications

(19 citation statements)

References 48 publications

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“…Hydroxyurea (HU), a United States Food and Drug Administration-approved drug [1], is an inhibitor of ribonucleotide reductase commonly used to treat myeloproliferative disorders and sickle cell anemia [2]. In addition, HU is used as an anti-tumor drug to treat various malignancies [3]. However, HU has several adverse effects and should be used with caution in pregnant women and children.…”

Section: Introductionmentioning

confidence: 99%

Hydroxyurea affects in vitro porcine oocyte maturation through increased apoptosis and oxidative stress

et al. 2021

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Hydroxyurea (HU) is an FDA-approved drug used to treat a variety of diseases, especially malignancies, but is harmful to fertility. We used porcine oocytes as an experimental model to study the effect of HU during oocyte maturation. Exposure of cumulus-oocyte complexes (COCs) to 20 µM (p < 0.01) and 50 µM (p < 0.001) HU reduced oocyte maturation. Exposure to 20 µM HU induced approximately 1.5-fold and 2-fold increases in Caspase-3 (p < 0.001) and P53 (p < 0.01) gene expression levels in cumulus cells, respectively, increased Caspase-3 (p < 0.01) and P53 (p < 0.001) protein expression levels in metaphase II (MII) oocytes and increased the percentage of apoptotic cumulus cells (p < 0.001). In addition, HU decreased the mitochondrial membrane potential (Δφm) (p < 0.01 and p < 0.001) and glutathione (GSH) levels (p < 0.01 and p < 0.001) of both cumulus cells and MII oocytes, while increasing their reactive oxygen species (ROS) levels (p < 0.001). Following parthenogenetic activation of embryos derived from MII oocytes, exposure to 20 µM HU significantly reduced total blastocyst cell numbers (p < 0.001) and increased apoptosis of blastocyst cells (p < 0.001). Moreover, HU exposure reduced the rate of development of 2-cell, 4 -8 cell, blastocyst, and hatching stages after parthenogenetic activation (p < 0.05). Our findings indicate that exposure to 20 µM HU caused significant oxidative stress and apoptosis of MII oocytes during maturation, which affected their developmental ability. These results provide valuable information for safety assessments of HU.

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Section: Introductionmentioning

confidence: 99%

Hydroxyurea affects in vitro porcine oocyte maturation through increased apoptosis and oxidative stress

et al. 2021

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“…The epididymal lesions were sloughing off some germinal epithelial in the lumen of some ducts of the epididymis, which indicate testicular malfunction [ 53 , 54 ] and interstitial congestion of blood vessel with perivascular inflammatory cell infiltrations; besides, most epididymal ducts seemed to have no or low sperm quantities in their lumen, which reflected the cessation of spermatogenesis. The prostates and seminal vesicle lesions experienced necrosis and desquamation of some glandular epithelial cells with low luminal discharges and severe interstitial leukocytes infiltration [ 53 ]. These alterations may be due to reduced testosterone, which needs differentiation, development, and the preservation of epithelial cells of accessory sex glands [ 55 ].…”

Section: Discussionmentioning

confidence: 99%

“…Another important finding in atrophied seminiferous tubules was noticed in our study due to the cytotoxic effect of FPN. The epididymal lesions were sloughing off some germinal epithelial in the lumen of some ducts of the epididymis, which indicate testicular malfunction [53,54] and interstitial congestion of blood vessel with perivascular inflammatory cell infiltrations; besides, most epididymal ducts seemed to have no or low sperm quantities in their lumen, which reflected the cessation of spermatogenesis. The prostates and seminal vesicle lesions experienced necrosis and desquamation of some glandular epithelial cells with low luminal discharges and severe interstitial leukocytes infiltration [53].…”

Section: Discussionmentioning

confidence: 99%

Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats

Tohamy

El-kazaz

Alotaibi

et al. 2021

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The study investigated the ability of boswellic acid (BA) to alleviate the testicular and oxidative injury FPN insecticide intoxication in the male rat model. Rats were randomly assigned to six equivalent groups (six rats each) as the following: control rats orally administered with 2 mL physiological saline/kg of body weight (bwt); boswellic acid (BA1) rats orally administered 250 mg BA/kg bwt; boswellic acid (BA2) rats orally administered 500 mg BA/kg bwt; fipronil (FPN) rats orally administered 20 mg FPN/kg bwt; (FPN + BA1) rats orally administered 20 mg FPN/kg bwt plus 250 mg BA/kg bwt, and (FPN + BA2) rats orally administered 20 mg FPN/kg bwt plus 500 mg BA/kg bwt. After 60 days, semen viability percentage and live spermatozoa percentage were decreased, and a considerably increased abnormality of the sperm cells in FPN-administered rats improved substantially with the co-administration of BA. BA had refinement of the histological architecture of testes and sexual glands. Quantitative analysis recorded a noticeable decline in the nuclear cell-proliferating antigen (PCNA) percentage area. FPN triggered cell damage, which was suggested by elevated malondialdehyde and interleukin 6, tumor necrosis factors alpha, and decreased glutathione level. Proapoptotic factor overexpression is mediated by FPN administration, while it decreased the antiapoptotic protein expression. Similarly, BA has shown significant upregulation in steroidogenic and fertility-related gene expression concerning the FPN group. Pathophysiological damages induced by FPN could be alleviated by BA’s antioxidant ability and antiapoptotic factor alongside the upregulation of steroidogenic and fertility-related genes and regimented the detrimental effects of FPN on antioxidant and pro-inflammatory biomarkers.

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“…Twenty-eight adult Wistar rats administered with royal jelly (100 mg/kg bw) for 6 weeks showed increases in CAT and FRAP activities [ 115 ]. Rats induced with hydroxylurea (225 or 450 mg kg/bw/day) followed by administration of royal jelly (100 mg kg/bw/day) for 60 days revealed improved sperm quality, hormonal, and antioxidant status as well as histology architecture [ 116 ] ( Table 5 ).…”

Section: Role Of Bee Products In Male Reproductive Impairmentmentioning

confidence: 99%

Review on Bee Products as Potential Protective and Therapeutic Agents in Male Reproductive Impairment

2021

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Bee products are sources of functional food that have been used in complementary medicine to treat a variety of acute and chronic illnesses in many parts of the world. The products vary from location to location as well as country to country. Therefore, the aim of this review was to identify various bee products with potential preventive and therapeutic values used in the treatment of male reproductive impairment. We undertook a vigorous search for bee products with preventive and therapeutic values for the male reproductive system. These products included honey, royal jelly, bee pollen, bee brood, apilarnil, bee bread, bee wax, and bee venom. We also explained the mechanisms involved in testicular steroidogenesis, reactive oxygen species, oxidative stress, inflammation, and apoptosis, which may cumulatively lead to male reproductive impairment. The effects of bee pollen, bee venom, honey, propolis, royal jelly, and bee bread on male reproductive parameters were examined. Conclusively, these bee products showed positive effects on the steroidogenic, spermatogenic, oxidative stress, inflammatory, and apoptotic parameters, thereby making them a promising possible preventive and therapeutic treatment of male sub/infertility.

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Attenuation potentials of royal jelly against hydroxyurea-induced infertility through inhibiting oxidation and release of pro-inflammatory cytokines in male rats

Cited by 13 publications

References 48 publications

Hydroxyurea affects in vitro porcine oocyte maturation through increased apoptosis and oxidative stress

Hydroxyurea affects in vitro porcine oocyte maturation through increased apoptosis and oxidative stress

Ameliorative Effects of Boswellic Acid on Fipronil-Induced Toxicity: Antioxidant State, Apoptotic Markers, and Testicular Steroidogenic Expression in Male Rats

Review on Bee Products as Potential Protective and Therapeutic Agents in Male Reproductive Impairment

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