Attenuation of liver mitochondrial oxidative damage by the extract and desulfo glucosinolate fraction of Lepidium perfoliatum L. seeds

Mirzaee, Fatemeh; Mohammadi, Hamidreza; Azarpeik, Sahar; Amiri, Fereshteh Talebpour; Shahani, Somayeh

doi:10.1016/j.sajb.2021.01.011

Cited by 8 publications

(4 citation statements)

References 46 publications

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“…Glucosinolates are sulfur- and nitrogen-rich secondary metabolites which could be distinguished into aliphatic GSL (AGS), indole GSL (IGS), and aromatic GSL(RGS) according to their amino acid precursors [ 9 , 10 ]. The desulfo glucosinolate fraction from Lepidium perfoliatum seed significantly reduced Reactive Oxygen Species (ROS) and Malondialdehyde (MDA) levels in liver mitochondria, which showed a significant hepatoprotective effect [ 11 ]. Blue light and UV-A at different intensities or wavelengths enhanced the GSL accumulation in red-leaf and green-leaf pak choi [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of Supplemental UV-A Intensity on Growth and Quality of Kale under Red and Blue Light

Jiang

et al. 2022

IJMS

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Different intensities of UV-A (6, 12, 18 μmol·m−2s−1) were applied in a plant factory to evaluate the combined influences of supplemental UV-A and red and blue light (Red:Blue = 1:1 at PPFD of 250 μmol·m−2 s−1) on the biomass, antioxidant activity and phytochemical accumulation of kale. Supplemental UV-A treatments (T1: 6 μmol·m−2 s−1, T2: 12 μmol·m−2 s−1 and T3: 18 μmol·m−2 s−1) resulted in higher moisture content, higher pigment content, and greater leaf area of kale while T2 reached its highest point. T2 treatment positively enhanced the antioxidant capacity, increased the contents of soluble protein, soluble sugar and reduced the nitrate content. T1 treatment markedly increased the content of aliphatic glucosinolate (GSL), whereas T2 treatment highly increased the contents of indolic GSL and total GSL. Genes related to GSL biosynthesis were down-regulated in CK and T3 treatments, while a majority of them were greatly up-regulated by T1 and T2. Hence, supplemental 12 μmol·m−2 s−1 UV-A might be a promising strategy to enhance the growth and quality of kale in a plant factory.

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Section: Introductionmentioning

confidence: 99%

Effect of Supplemental UV-A Intensity on Growth and Quality of Kale under Red and Blue Light

Jiang

et al. 2022

IJMS

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“…Here, the hydroalcoholic extract of LP was examined against T. gondii (TG) in vitro. Our data revealed that glucoerucin protects LP from CCl 4-mediated hepatic destruction via suppression of the oxidative damage networks [7]. The water extract of L. virginicum (LV) contains anti-cancer and anti-in ammatory properties.…”

Section: Introductionmentioning

confidence: 79%

A Comparative Study of the Chloroplast Genomes of Five Lepidium Medicinal Plants

Zhou¹,

Chen²,

Ming-yuan³

et al. 2021

Preprint

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Background Herb genomics is a rapidly developing field of medicinal plant research and development. Plant genomic studies demonstrate the unique advantage of employing plants in medicinal therapy. The genus Lepidium falls under the Brassicaceae family and it includes crucial medicinal plants. Herein, we sequenced the complete chloroplast (cp) genomes of Lepidium apetalum (LA) and Lepidium perfoliatum (LP) and assessed their genetic profiles against the reported profiles of Lepidium sativum (LS), Lepidium meyenii (LM), and Lepidium virginicum (LV). Results In particular, we examined genomic arrangement, gene number, type, and repeat sequences. Based on our annotation data, both LA and LP possessed 130 distinct genes that included 85 protein-coding, 37 transfer RNA (tRNA), and 8 ribosomal RNA (rRNA) genes. Our repeat analyses revealed that LA harbored 20 forward repeats, 16 palindrome repeats, 30 tandem repeats, and 87 simple sequence repeats, whereas LP had 15 forward repeats, 20 palindrome repeats,4 reverse repeats, 21 tandem repeats, and 98 simple sequence repeats. Using syntenic analysis, we also revealed a high degree of sequence similarity within the coding regions of Lepidium cp genomes and a remarkably high degree of divergence among the intergenic spacers. Pairwise alignment and single-nucleotide polymorphism (SNP) examinations further revealed certain Lepidium-specific gene fragments, particularly in the intergenic regions of the trnK-atpA, trnC-psbC, trnT-rbcL, ndhF-ndhH, ycf1-trnR, accD, ccsA, matK, ndhF, rpoB, rpoC2, and ycf1 genes. Moreover, following codon usage analysis, we observed that codon 14 was the most frequently used codon in the Lepidium CDS. In addition, correlation investigations revealed that the ENC (the effective number of codon) content was strongly associated with GC3, GC3s, and N. Conclusion Based on these data, LA and LP originate from very similar genetic backgrounds. Furthermore, neutrality, ENC, and PR2-plots analyses demonstrated that the CUB (the codon usage bias) of Lepidium cp genome was strongly influenced by mutation and natural selection. Our analysis of the cp genomic sequences of LA and LP will likely enhance breeding, species recognition, phylogenetic evolution, and cp genetic engineering of the Lepidium medicinal plants.

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“…5'6-dimethoxy-2',3'-methylenedioxy-7-C-β-D-glucopyranosyl isoflavone (an isoflavone C-glycoside) was isolated from LS seeds, which reduces serum lipids profile, free radicals, and improves hepatic function in hepatotoxicity with paracetamol [27]. Also, the desulfo-glucosinolate fraction of L. perfoliatum seeds (glucoerucin was the major ingredient) improved hepa- totoxicity by increasing GSH and decreasing Reactive Oxygen Species (ROS), and malondialdehyde [28]. Inflammation is one of the most important factors in liver toxicity.…”

Section: Discussionmentioning

confidence: 99%

Effects of Hydroalcoholic Extract of Lepidium Sativum L. on Carbon Tetrachloride-Induced Hepatotoxicity in Mice

Shokrzadeh¹,

Khalvati²,

Hosseinzadeh³

et al. 2022

PBR

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Background: Carbon tetrachloride (CCl4 ) as an organic solvent causes symptoms of acute and chronic liver injury, including necrosis, fat changes, liver cancer, and cirrhosis. Lepidium sativum contains flavonoids, alkaloids, and antioxidant components. Objectives: This study aims to investigate the hepatic protection of L. Sativum Extract (LSE) on CCl4 -induced hepatotoxicity in mice. Methods: A total of 25 male mice were randomly divided into five groups (n=5): control (olive oil), CCl4 , and 3 LSE groups. Except for the control group, all the mice received CCl4 (50%, 0.5 mL/kg) intraperitoneally twice a week for 4 weeks. The mice in the LSE groups were treated daily with LSE (200, 400, and 600 mg/kg) via IP injection. The animals were sacrificed 24 h after the last dose, and liver function parameters, such as Alkaline Phosphatase (ALP), Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), and Glutathione (GSH) were determined. Furthermore, 0.1 g of liver tissue was removed for histochemical analysis. Results: Significant differences were observed in GSH, ALP, AST, and ALT levels between the CCI4 and the control groups. Compared to the CCl4 group, LSE treatment significantly decreased plasma ALT (P<0.05), AST in all doses (P<0.001), and ALP in 600 mg/kg (P<0.001). In addition, LSE treatment significantly increased GSH in 400 mg/kg (P<0.01) and 600 mg/kg (P<0.001). Conclusion: LSE has hepatic protective activity against CCl4 -induced injuries. The possible anti-hepatotoxic mechanisms may be related to the presence of flavonoids, triterpenes, alkaloids, tannin, and coumarins in the LSE by inhibiting the free radicals mediated damage.

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Attenuation of liver mitochondrial oxidative damage by the extract and desulfo glucosinolate fraction of Lepidium perfoliatum L. seeds

Cited by 8 publications

References 46 publications

Effect of Supplemental UV-A Intensity on Growth and Quality of Kale under Red and Blue Light

Effect of Supplemental UV-A Intensity on Growth and Quality of Kale under Red and Blue Light

A Comparative Study of the Chloroplast Genomes of Five Lepidium Medicinal Plants

Effects of Hydroalcoholic Extract of Lepidium Sativum L. on Carbon Tetrachloride-Induced Hepatotoxicity in Mice

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