Attenuation of DNA charge transport by compaction into a nucleosome core particle

Bjorklund, Chad C.; Davis, William B.

doi:10.1093/nar/gkl030

Cited by 28 publications

(53 citation statements)

References 54 publications

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“…Thus it appears that even within the nucleosome, DNA CT may proceed. This long range CT within DNA in the nucleosome core particle was confirmed in similar experiments using tethered anthraquinone as the photooxidant [31]. Some variations in relative intensities across the guanine doublets were observed for damage in the nucleosome versus that for the free DNA when comparing anthraquinone and the Rh intercalator.…”

Section: Long Range Ct In the Presence Of Dna-bound Proteinssupporting

confidence: 61%

Biological contexts for DNA charge transport chemistry

Merino

Boal

Barton

2008

Current Opinion in Chemical Biology

114

105

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SUMMARYMany experiments have now shown that double helical DNA can serve as a conduit for efficient charge transport (CT) reactions over long distances in vitro. These results prompt the consideration of biological roles for DNA-mediated CT. DNA CT has been demonstrated to occur in biologically relevant environments such as within the mitochondria and nuclei of HeLa cells as well as in isolated nucleosomes. In mitochondria, DNA damage that results from CT is funneled to a critical regulatory element. Thus DNA CT provides a strategy to funnel damage to particular sites in the genome. DNA CT might also be important in long range signaling to DNA-bound proteins. Both DNA repair proteins, containing Fe-S clusters, and the transcription factor, p53, which is regulated through thiol-disulfide switches, can be oxidized from a distance through DNA-mediated CT. These observations highlight a means through which oxidative stress may be chemically signaled in the genome over long distances through CT from guanine radicals to DNAbound proteins. Moreover, DNA-mediated CT may also play a role in signaling among DNAbinding proteins, as has been proposed as a mechanism for how DNA repair glycosylases more efficiently detect lesions inside the cell.

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Section: Long Range Ct In the Presence Of Dna-bound Proteinssupporting

confidence: 61%

Biological contexts for DNA charge transport chemistry

Merino

Boal

Barton

2008

Current Opinion in Chemical Biology

114

105

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“…These findings are in agreement with previously reported results. [22] Similar situations can be observed in the LysH + -G or HisH + -G complexes ( Figures S35-S38). …”

supporting

confidence: 69%

“…[15,16] For example, G · + reacts with H 2 O or a strong chemical oxidant at certain sites, giving rise to 8-oxo-7,8-dihydroguanine and other G lesions products. [17][18][19][20] Many efforts have been devoted to explore the protective mechanism of damaged G. The main strategies, which include repairing damaged DNA, [21] packaging DNA with lipid-like compounds, [22] and driving the radical cations away from the coding regions [23] have been described in detail.…”

Section: Introductionmentioning

confidence: 99%

Interactions of Amino Acids with Oxidized Guanine in the Gas Phase Associated with the Protection of Damaged DNA

et al. 2013

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Density functional theory calculations were employed to study the stabilization process of the guanine radical cation through amino acid interactions as well as to understand the protection mechanisms. On the basis of our calculations, several protection mechanisms are proposed in this work subject to the type of the amino acid. Our results indicate that a series of three-electron bonds can be formed between the amino acids and the guanine radical cation which may serve as relay stations supporting hole transport. In the three-electron-bonded, π-π-stacked, and H-bonded modes, amino acids can protect guanine from oxidation or radiation damage by sharing the hole, while amino acids with reducing properties can repair the guanine radical cation through proton-coupled electron transfer or electron transfer. Another important finding is that positively charged amino acids (ArgH(+), LysH(+), and HisH(+)) can inhibit ionization of guanine through raising its ionization potential. In this situation, a negative dissociation energy for hydrogen bonds in the hole-trapped and positively charged amino acid-Guanine dimer is observed, which explains the low hole-trapping efficiency. We hope that this work provides valuable information on how to protect DNA from oxidation- or radiation-induced damages in biological systems.

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“…Packaging DNA with lipid-like compounds provides some protection [20]. Low-oxidation-potential sacrificial donors such as a disulfide or an 8-oxoG will protect guanines located nearby.…”

Section: Protective Effect Of 8-oxog On the Reaction And Transportmentioning

confidence: 99%

Oxidative damage to DNA: Inhibition of guanine damage

Kanvah

Schuster

2006

Pure and Applied Chemistry

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One-electron oxidation of DNA results in chemical damage to nucleobases, particularly guanine in multiple G sequences. Oxidation may be triggered by numerous events, including photosensitization. We describe studies of photoinduced oxidations of DNA triggered by irradiation of covalently linked anthraquinone derivatives under various conditions that affect the global structure of the DNA. These structural changes have subtle effects on the result of the one-electron oxidation.

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Attenuation of DNA charge transport by compaction into a nucleosome core particle

Cited by 28 publications

References 54 publications

Biological contexts for DNA charge transport chemistry

Biological contexts for DNA charge transport chemistry

Interactions of Amino Acids with Oxidized Guanine in the Gas Phase Associated with the Protection of Damaged DNA

Oxidative damage to DNA: Inhibition of guanine damage

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