Attenuation of contractile dysfunction by atorvastatin after intestinal ischemia reperfusion injury in rats

Özaçmak, Veysel Haktan; Sayan, Hale; Igdem, Aysenur Akyildiz; Çetin, Abdurrahman; Ozacmak, I. Diler

doi:10.1016/j.ejphar.2007.01.061

Cited by 26 publications

(18 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, OFRs lead to lipid peroxidation within the cell membranes by reacting directly with polyunsaturated fatty acids. Various studies have demonstrated that OFRs cause tissue damage in the intestinal tissue subjected to I/R [30]. In the present study, the intestinal content of MDA, a product of lipid peroxidation, was significantly increased in the I/R group compared to the control.…”

Section: Discussionsupporting

confidence: 56%

The Effects of Amlodipine on the Biochemical and Histopathological Changes in the Rabbit Ileum Subjected to Ischemia-Reperfusion

Coşkun¹,

Günal²,

Halıcı³

et al. 2011

EAJM

View full text Add to dashboard Cite

Objective: The aim of this study was to determine the potential, protective effects of amlodipine in an experimental, ischemia-reperfusion (I/R) model in the rabbit small intestine. Materials and Methods:The rabbits were divided into four groups: sham-operated, amlodipine (10 mg/kg) + sham-operated, I/R, and I/R + amlodipine (10 mg/kg) groups. An intestinal I/R model was applied to the rabbits. The superior mesenteric artery was occluded for 1 h with an atraumatic vascular clamp and then was reperfused for 2 h. Animals in the amlodipine and I/R + amlodipine groups received the amlodipine by oral gavage. At the end of the 2-h-reperfusion period, the animals were sacrificed.Results: Pretreatment with amlodipine significantly increased SOD activity and GSH levels to values close to those found in the serum from the I/R group. Rabbits in the I/R group showed high levels of serum MDA. Amlodipine pretreatment significantly reduced the serum MDA levels compared to the I/R group, although the MDA levels in the I/R + amlodipine group were still higher than in the sham-operated group. The I/R damage was ameliorated by amlodipine pretreatment, as evidenced by histopathological analysis. Conclusion:The present study is the first to report an attenuation of I/Rinduced intestinal injury by the systemic administration of amlodipine.Key Words: Amlodipine, Ischemia-Reperfusion, Glutathione, Superoxide Dismutase, Malonyldialdehyde, Rabbit Özet Amaç: Biz bu çalışmada, tavşan ince barsak iskemi-reperfüzyon (I/R) deneysel modeli üzerine amlodipin'in koruyucu etkisini inceledik. Gereç ve Yöntem:Tavşanlar dört guruba ayrıldı: şam operasyon gurubu, 10mg/kg amlodipin+şam operasyon gurubu, I/R gurubu, I/R+ 10 mg/kg amlodipin gurubu. İntestinal iskemi/reperfüzyon modeli tavşanlara uygulanıldı. Superior mezenterik arter 1 saat sure ile atravmatik vasküler klemp ile klempe edildi sonrasında 2 saat reperfüzyon uygulanıldı. Amlodipin ve amlodipin+I/R guruplarına oral gavaj ile amlodipin verildi. İki saatlik reperfüzyon süresinin sonunda hayvanlar sakrifiye edildi. I/R hasarının amlodipin uygulaması ile düzeldiği histopatolojik analizler ile ispatlandı. Bulgular: Amlodipin uygulaması I/R 'da serumda bulunan SOD aktivitesi ve GSH seviyesini anlamlı şekilde artırdı. Tavşanlarda intestinal I/R'ında yüksek seviyede MDA gözlendi. Amlodipin uygulaması intestinal I/R bulunan tavşan serumunda MDA aktivite seviyesini anlamlı şekilde düşürürken bu düşüş I/R+amlodipin gurubu control gurubu ile mukayese edildiğinde çok daha fazlaydı. Sonuç:Mevcut çalışma I/R ile oluşan intestinal hasar üzerine amlodipin'in sistemik uygulamasının iyleştirici etkisini gösteren ilk çalışmadır.

show abstract

Section: Discussionsupporting

confidence: 56%

The Effects of Amlodipine on the Biochemical and Histopathological Changes in the Rabbit Ileum Subjected to Ischemia-Reperfusion

Coşkun¹,

Günal²,

Halıcı³

et al. 2011

EAJM

View full text Add to dashboard Cite

show abstract

“…MDA is a final product of lipid peroxide, and its concentration in tissues can directly reflect the extent of lipid peroxide injury [20] . The concentration of MDA in each group gradually increased after reperfusion, which is generally considered the result of generous release of free radicals and accumulation of lipid peroxide products after pan-intestinal IR [21] . However, the concentration of MDA was obviously lower in the melatonin exposure group than in the other two control groups 6, 12 and 24 h after reperfusion, indicating that melatonin can relieve the IR injury by eliminating free radicals [22] .…”

Section: Applicationsmentioning

confidence: 98%

Melatonin protects liver from intestine ischemia reperfusion injury in rats

Li¹,

Yin²,

Gu³

et al. 2008

WJG

View full text Add to dashboard Cite

AIM:To investigate the protective effect of melatonin on liver after intestinal ischemia-reperfusion injury in rats. METHODS: One hundred and fifty male Wistar rats, weighing 190-210 g, aged 7 wk, were randomly divided into melatonin exposure group, alcohol solvent control group and normal saline control group. Rats in the melatonin exposure group received intraperitoneal (IP) melatonin (20 mg/kg) 30 min before intestinal ischemia-reperfusion (IR), rats in the alcohol solvent control group received the same concentration and volume of alcohol, and rats in the normal saline control group received the same volume of normal saline. Serum samples were collected from each group 0.5, 1, 6, 12, and 24 h after intestinal IR. Levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured with an auto-biochemical analyzer. Serum TNF-α was tested by enzyme-linked immunosorbent assay (ELISA). Malondialdehyde (MDA) in liver was detected by colorimetric assay. Pathological changes in liver and immunohistochemical straining of ICAM-1 were observed under an optical microscope. RESULTS: The levels of ALT measured at various time points after intestinal IR in the melatonin exposure group were significantly lower than those in the other two control groups (P < 0.05). The serum AST levels 12 and 24 h after intestinal IR and the ICAM-1 levels (%) 6, 12 and 24 h after intestinal IR in the melatonin exposure group were also significantly lower than those in the other two control groups (P < 0.05). CONCLUSION: Exotic melatonin can inhibit the activity of ALT, AST and TNF-α, decrease the accumulation of MDA, and depress the expression of ICAM-1 in liver after intestinal IR injury, thus improving the liver function.

show abstract

“…However, statins are known to decrease inflammation, directly decreasing the release of pro-inflammatory cytokines, including IL-6, IL-8, and TNF-a [35][36][37]. Moreover, statins decrease oxidative stress by altering the balance between endothelial nitric oxide synthase and inducible nitric oxide synthase, thus protecting against endothelial cell dysfunction and bacterial translocation [25,32,38,39]. Preservation of endothelial cell integrity decreases leucocyte extravasation within the peritoneum and has been shown to lead to survival benefits in experimental models of abdominal sepsis and colitis [38,[40][41][42].…”

Section: Discussionmentioning

confidence: 99%