Attenuation of Allergic Immune Response Phenotype by Mannosylated Egg White in Orally Induced Allergy in Balb/c Mice

Abstract

Food allergies are attributed to an imbalance in immune response to ubiquitous antigens. A previous study demonstrated that mannose glycation (mannosylation) of ovalbumin decreased allergenicity in vivo. The proposed research targets mannosylation of various common allergens that may help prevent food allergy. Balb/c mice (n = 8) were sensitized toxin egg white, peanut, and whey and treated with mannosylated forms of the test antigens. Glucosylated peanut and cholera toxins were used as controls. Allergic status was assessed as clinical signs, serum histamine, mouse mast cell protease (MMCP), antibody activity, cytokines, and T regulatory cells (T-regs). Significant preventative effects were observed with mannosylated egg white treatment such as reduced clinical signs, histamine, MMCP, specific G, G1, and E antibody activities, and IL-4 and increased IL-10 and CD25(+) Foxp3(+) cells. Other groups did not differ significantly. It was concluded that mannosylated egg white provides a powerful tool to prevent allergic phenotypes with possible relevance to control human egg allergy.

Diets Supplemented with 1% Egg White Induce Oral Desensitization and Immune Tolerance in an Egg White-Specific Allergic Mouse Model

“…In previous reports, the amount of allergen needed to induce oral desensitization in mice was approximately 1–5 mg [8-11]. In this study, because the daily food intake was approximately 2.7 g for each mouse (online suppl.…”

“…S1). Previous mouse studies have used cholera toxin as the oral adjuvant [8-11]; in contrast, we used alum as an intraperitoneal adjuvant. Different allergic mouse models are thought to require different minimum allergen amounts to induce oral desensitization.…”

“…Although pervious OIT studies in mice were administrated by oral gavage [8-11], the experimental OIT in mice can continuously ingest small amounts of allergen. The merits of this OIT method are: (1) the dose of the allergen is divided, (2) the dose of the allergen ingested can be easily adjusted, and (3) the taste and smell of the allergen are masked.…”

“…We reported that diet intake that includes 1% OM (30 mg/24 h/mouse) for 4 weeks induces oral desensitization in an OM-specific allergic mouse model [7]. Results of previous studies in mice suggested that 1–5 mg of allergen is needed to induce oral desensitization by oral gavage [8-11]. However, the minimum dietary concentration required to elicit oral desensitization was not clear.…”

Oral Immunotherapy with a Phosphorylated Hypoallergenic Allergen Ameliorates Allergic Responses More Effectively Than Intact Allergen in a Murine Model of Buckwheat Allergy

“…In a recent study, Rupa et al. reported that mannosylation effectively suppressed orally induced allergic immune responses to egg white proteins, but not whey or peanut proteins, in mice . Specifically, this mannosylation resulted in increased populations of Treg cells, but no changes in IgA levels.…”