Attenuating effects of coenzyme Q10 and amlodipine in ulcerative colitis model in rats

Morsy, Engy M. El; Kamel, Rehab; Ahmed, Maha A.E.

doi:10.3109/08923973.2015.1021357

Cited by 37 publications

(21 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, at present, it is not possible to align the multiple contradicting results of the reported clinical trials. On the other side, a plethora of literature supports the role of oxidative stress in UC and consequently justifies the use of antioxidants as a therapeutic strategy [15,27,52,54,60,[98][99][100][101][102]. Since UC is a multifactorial disease, pharmacological targeting of individual factors appears of limited use to provide effective and sustainable therapy outcomes.…”

Section: Discussionmentioning

confidence: 99%

Idebenone Protects against Acute Murine Colitis via Antioxidant and Anti-Inflammatory Mechanisms

Shastri

Shinde

Sohal

et al. 2020

IJMS

View full text Add to dashboard Cite

Oxidative stress is a key player of the inflammatory cascade responsible for the initiation of ulcerative colitis (UC). Although the short chain quinone idebenone is considered a potent antioxidant and a mitochondrial electron donor, emerging evidence suggests that idebenone also displays anti-inflammatory activity. This study evaluated the impact of idebenone in the widely used dextran sodium sulphate (DSS)-induced mouse model of acute colitis. Acute colitis was induced in C57BL/6J mice via continuous exposure to 2.5% DSS over 7 days. Idebenone was co-administered orally at a dose of 200 mg/kg body weight. Idebenone significantly prevented body weight loss and improved the disease activity index (DAI), colon length, and histopathological score. Consistent with its reported antioxidant function, idebenone significantly reduced the colonic levels of malondialdehyde (MDA) and nitric oxide (NO), and increased the expression of the redox factor NAD(P)H (nicotinamide adenine dinucleotide phosphate) dehydrogenase quinone-1 (NQO-1) in DSS-exposed mice. Immunohistochemistry revealed a significantly increased expression of tight junction proteins, which protect and maintain paracellular intestinal permeability. In support of an anti-inflammatory activity, idebenone significantly attenuated the elevated levels of pro-inflammatory cytokines in colon tissue. These results suggest that idebenone could represent a promising therapeutic strategy to interfere with disease pathology in UC by simultaneously inducing antioxidative and anti-inflammatory pathways.

show abstract

Section: Discussionmentioning

confidence: 99%

Idebenone Protects against Acute Murine Colitis via Antioxidant and Anti-Inflammatory Mechanisms

Shastri

Shinde

Sohal

et al. 2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Our findings are agreement with many studies. CoQ 10 significantly attenuated TNF-α level in ulcerative colitis in rats [7]. On the other hand, IL-6 and TNF-α levels were decreased by CoQ 10 application in patients with multiple sclerosis [24].…”

Section: Discussionmentioning

confidence: 92%

“…This function was accomplished either directly via protecting cellular components from free radicals or indirectly through regeneration of other endogenous antioxidants [4,5]. In experimental studies, it has been demonstrated antioxidant, anti-inflammatory and ATP-regenerative properties of CoQ 10 in various diseases such as gastric ulcer, osteoarthritis and diabetes [6,7,15,16].…”

Section: Introductionmentioning

confidence: 99%

The Effects of Coenzyme Q10 on Inflammation Markers in Streptozotocin-Induced Diabetic Rats

Uluişik

Keskin

2017

Acta Scientiae. Vet.

View full text Add to dashboard Cite

Background: Coenzyme Q10 is a well-known cofactor in the mitochondrial electron transport chain required for ATP production. Coenzyme Q10 is recognized as an intracellular antioxidant that protects cell membrane phospholipids, mitochondrial membrane protein, and plasma low-density lipoprotein against oxidative damage caused by free radicals. Diabetes and its complications have been related to increased levels of free radicals and systemic proinflammatory cytokines and to an abnormal lipid profile. The aim of this study was to investigate the effects of coenzyme Q10 supplementation on some cytokine levels in streptozotocin-induced diabetic rats.Materials, Methods & Results: In this study, 38 healthy, adult male rats were used. The rats were divided into 5 groups. All animals were housed in separated cages during the four weeks. The animals in group 1 was fed standard rat pellets for 4 weeks. It was administered at 0.3 mL corn oil intraperitoneally daily for four weeks in group 2 animals. The animals in group 3 was injected intraperitoneally with 10 mg/kg CoQ10 daily for 4 weeks. Group 4 was made diabetic by subcutaneous injections of streptozotocin at dose of 40 mg/kg in 0.1 M citrate buffer (pH 4.5) single daily dose for two days and group 5 was made diabetic by subcutaneous injections of streptozotocin at dose of 40 mg/kg in 0.1 M citrate buffer (pH 4.5) single daily dose for two days and then was injected intraperitoneally with 10 mg/kg CoQ10 daily for 4 weeks. During the experiment, three animals from group 4 and one animals from group 5 were died due to streptozotocin-induced hypoglycemia. At the end of the study, blood samples were taken from all animals. In these blood samples, IL-4, IL-6, IL-10 and TNF-α plasma levels were determined with ELISA using sandwich enzyme-linked immunosorbent method via commercial kits. In this study, IL-4 level as an anti-inflammatory cytokine significantly decreased (P < 0.05) with diabetes induction compared to control group level. IL-10 level in diabetic group was statistically different (P < 0.05) from control group level. CoQ10 application to diabetic animals improved the falling in IL-10 level of diabetic group (P < 0.05). IL-6 and TNF-α levels in diabetic group significantly increased (P < 0.05) in parallel with each other compared to control group levels. The same parameters were reduced (P < 0.05) by CoQ10 application in diabetic animals.Discussion: In this study, the occurred changes in pro- and anti-inflammatory cytokines with experimentally induced diabetes are expected results and these results are consistent with some studies related diabetes. These results may be considered to hazardous effects and inflammation caused by diabetes on liver, pancreas and other tissues. CoQ10 suppressed the increments in plasma pro-inflammatory cytokine levels, whereas it restored the reducing in anti-inflammatory cytokine levels arising due to diabetes. The obtained results from this study after CoQ10 application supported similar studies used CoQ10 application against deleterious effects of diabetes in animals and humans. Therefore, it is possible to say that CoQ10 may play important role in regulation of imbalance between inflammation markers in diabetes conditions and further studies are needed to clear the beneficial effects of CoQ10 treatment on the other inflammation markers in diabetes status.

show abstract

“…TNF- α , PGE 2 , and IL-6 also play an important role in the inflammatory injury involved in UC ( Yamamoto et al, 2000 ; Gombošová et al, 2011 ; El Morsy et al, 2015 ; Sadar et al, 2016 ; El-Ashmawy et al, 2017 ; Gawrońska et al, 2017 ; Rafa et al, 2017 ; Li et al, 2018 ; Loynes et al, 2018 ). Moreover, GO analysis indicated that the proteins regulated by SNT in order to mediate its effects of UC play important roles in the pathogenesis of UC by regulating neutrophil degranulation, cell secretion, and other biological pathways.…”

Section: Discussionmentioning

confidence: 99%

Systematic Investigation of the Efficacy of Sinitang Decoction Against Ulcerative Colitis

Wang

et al. 2020

Front. Pharmacol.

View full text Add to dashboard Cite

The aim of this study was to investigate the precise clinical use of Sinitang decoction (SNT) in ulcerative colitis (UC). Network pharmacology-based analysis of the drug components–targets–diseases–pathways was used to predict the possible clinical applications of SNT. Next, 2,4,6-trinitrobenzenesulfonic acid (TNBS) was used to establish a rat model of UC, and the efficacy of SNT against UC was tested, followed by a proteomic analysis of the specific signatures regulated by SNT against UC. SNT was predicted to be effective in inflammatory bowel disease, UC, and several other diseases. In the rats with UC, SNT decreased the disease activity index and colon mucosal damage index compared to the untreated UC model rats. Additionally, SNT reversed the upregulated levels of serum tumor necrosis factor (TNF)-α, prostaglandin E 2 (PGE 2 ), interleukin (IL)-6, and nitric oxide (NO) in UC model rats. The proteomic analysis identified 78 proteins that were differentially regulated by SNT in the rats with UC, which were associated with the Gene Ontology terms sulfur compound binding, calcium ion binding, and Toll-like receptor (TLR)-4 binding. Among these differentially regulated proteins, C-reactive protein (CRP) and collagen alpha-1(XII) chain (COL12A1) were found to be signature proteins associated with the efficacy of SNT against UC. This study represents the first precise investigation of the efficacy and mechanisms of SNT against UC, and shows that SNT is a promising candidate for personalized management of UC.

show abstract

Attenuating effects of coenzyme Q10 and amlodipine in ulcerative colitis model in rats

Cited by 37 publications

References 51 publications

Idebenone Protects against Acute Murine Colitis via Antioxidant and Anti-Inflammatory Mechanisms

Idebenone Protects against Acute Murine Colitis via Antioxidant and Anti-Inflammatory Mechanisms

The Effects of Coenzyme Q10 on Inflammation Markers in Streptozotocin-Induced Diabetic Rats

Systematic Investigation of the Efficacy of Sinitang Decoction Against Ulcerative Colitis

Contact Info

Product

Resources

About