Attenuated phagocytic activity of monocytes in type 2 diabetic Goto–Kakizaki rats

Takeda, Yuji; Marumo, Mikio; Wakabayashi, Ichiro

doi:10.1016/j.imbio.2011.05.003

Cited by 10 publications

(9 citation statements)

References 56 publications

(59 reference statements)

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“…Additionally, the samples were stored at −20°C in methanol until the flow cytometry measurements were obtained. These advances simplify the procedure [55, 57, 73]. …”

Section: Measurement Of Monocyte Response and Amplification Circuitsmentioning

confidence: 99%

Relationship between Immunological Abnormalities in Rat Models of Diabetes Mellitus and the Amplification Circuits for Diabetes

Takeda

Shimomura

Asao

et al. 2017

Journal of Diabetes Research

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A better understanding of pathogenic mechanisms is required in order to treat diseases. However, the mechanisms of diabetes mellitus and diabetic complications are extremely complex. Immune reactions are involved in the pathogenesis of diabetes and its complications, while diabetes influences immune reactions. Furthermore, both diabetes and immune reactions are influenced by genetic and environmental factors. To address these issues, animal models are useful tools. So far, various animal models of diabetes have been developed in rats, which have advantages over mice models in terms of the larger volume of tissue samples and the variety of type 2 diabetes models. In this review, we introduce rat models of diabetes and summarize the immune reactions in diabetic rat models. Finally, we speculate on the relationship between immune reactions and diabetic episodes. For example, diabetes-prone Biobreeding rats, type 1 diabetes model rats, exhibit increased autoreactive cellular and inflammatory immune reactions, while Goto-Kakizaki rats, type 2 diabetes model rats, exhibit increased Th2 reactions and attenuation of phagocytic activity. Investigation of immunological abnormalities in various diabetic rat models is useful for elucidating complicated mechanisms in the pathophysiology of diabetes. Studying immunological alterations, such as predominance of Th1/17 or Th2 cells, humoral immunity, and innate immune reactions, may improve understanding the structure of amplification circuits for diabetes in future studies.

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“…Additionally, the samples were stored at −20°C in methanol until the flow cytometry measurements were obtained. These advances simplify the procedure [55, 57, 73]. …”

Section: Measurement Of Monocyte Response and Amplification Circuitsmentioning

confidence: 99%

Relationship between Immunological Abnormalities in Rat Models of Diabetes Mellitus and the Amplification Circuits for Diabetes

Takeda

Shimomura

Asao

et al. 2017

Journal of Diabetes Research

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“…CD11b has been associated with high-fat diet-induced obesity [37], and carotid intima-media thickness [38], and CD11b expression was described as lowest for non-classical monocytes after lipopolysaccharide stimulation [39] and in a rodent model of type 2 diabetes [40]. Results concerning CD36 atherogenicity are also contradictory since an increase [35] or deficiency [41] of its expression have been associated with the pathogenesis of atherosclerosis and CVD.…”

Section: Discussionmentioning

confidence: 99%

Predictors of Subclinical Inflammatory Obesity: Plasma Levels of Leptin, Very Low-Density Lipoprotein Cholesterol and CD14 Expression of CD16+ Monocytes

Leite

Santos

et al. 2017

Obes Facts

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Objective: Predictors of subclinical inflammatory obesity (SIO) can be important tools for early therapeutic interventions in obesity-related comorbidities. Waist circumference (WC) and BMI have different SIO sensitivity. We aimed to i) identify SIO predictors and ii) investigate whether CD16+ monocytes are associated with BMI- (generally) or WC-defined (centrally) obesity. Methods: Anthropometric and metabolic/endocrine (namely catecholamines, adrenaline and noradrenaline) parameters were evaluated, and CD16+ monocytes were studied by flow cytometry in the peripheral blood from 63 blood donors, and compared and correlated to each other. Multiple linear regression analysis was performed to identify variables that best predict SIO. Results: CD16+ monocyte counts were similar in BMI and WC groups. CD16+ monocytes from centrally obese (CO) showed a more inflammatory pattern, as compared to non-CO subjects. WC was sensitive to lipidemia and, in CO subjects, lipidemia was associated with a more inflammatory phenotype of CD16+ monocytes. These differences were not noticed between BMI groups. Adrenaline was correlated with CD16+ monocyte expansion with a lower inflammatory pattern. Leptin, very low-density lipoprotein cholesterol (VLDL-C), and CD14 expression of CD16+ monocytes were found to be CO predictors. Conclusions: WC-, but not BMI-defined obesity, was associated with a more inflammatory pattern of CD16+ monocytes, without monocyte expansion, suggesting that a monocyte maturation process rather than an independent arise of CD16+ monocytes occurs in CO. Thus, in a population with low cardiovascular risk, leptin, VLDL-C, and CD14 expression of CD16+ monocytes predict CO, constituting a putative tool for screening of SIO.

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“…The decreased perfusion also limits the total number of immune cells delivered to the infection site (12). Diabetes has been shown to impair cellular and humoral immunity, which is characterized by abnormalities in antibody titers associated with possible B-cell dysfunction and impairments in margination, chemotaxis, phagocytosis, and bactericidal functions, which are vital in immune defense (13)(14)(15)(16)(17)(18).…”

Section: Discussionmentioning

confidence: 99%

Is there any difference in tetanus IgG levels of diabetic patientswith respect to the presence of foot ulcers?

Teki̇n¹,

Sürmeli

Çizmeci

et al. 2017

Turk J Med Sci

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The aim of this study was to reveal the tetanus immunization status of diabetic patients and to determine whether diabetic patients with foot ulcers have different TIG levels. Materials and methods:A cross-sectional study was designed that included diabetic patients with foot ulcers (n = 30) and diabetic patients without ulcers (n = 30). The groups were compared for serum TIG levels along with total serum protein, albumin, C-reactive protein (CRP), and total immunoglobulin G (Ig G).Results: For diabetic patients without foot ulcers, 17 of 30 (56.6%) patients were found to have nonprotective TIG levels whereas for diabetic patients with foot ulcers, 28 of 30 (93.3%) patients were found to have nonprotective TIG levels. The mean value of TIG for diabetic patients without foot ulcers was 0.345 ± 0.281 IU/mL and for diabetic patients with foot ulcers the mean TIG value was 0.055 ± 0.033 IU/mL. Statistically significant differences were observed in TIG (P = 0.008), total protein (P < 0.001), albumin (P < 0.001), and CRP levels (P < 0.001) between the two groups. Conclusion:The majority of the diabetic patients had low TIG levels and they were significantly lower in diabetic patients with ulcers. A booster dose of tetanus vaccine should be considered for diabetic patients with and without diabetic foot ulcers.

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Attenuated phagocytic activity of monocytes in type 2 diabetic Goto–Kakizaki rats

Cited by 10 publications

References 56 publications

Relationship between Immunological Abnormalities in Rat Models of Diabetes Mellitus and the Amplification Circuits for Diabetes

Relationship between Immunological Abnormalities in Rat Models of Diabetes Mellitus and the Amplification Circuits for Diabetes

Predictors of Subclinical Inflammatory Obesity: Plasma Levels of Leptin, Very Low-Density Lipoprotein Cholesterol and CD14 Expression of CD16+ Monocytes

Is there any difference in tetanus IgG levels of diabetic patientswith respect to the presence of foot ulcers?

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