Attenuated amiloride-sensitive current and augmented calcium-activated chloride current in marsh rice rat (<i>Oryzomys palustris</i>) airways

Kuan, Shin‐Ping; Liao, Yan‐Shin; Davis, Katelyn M.; Messer, Jonathan G.; Zubcevic, Jasenka; Aguirre, J. Ignacio; Reznikov, Leah R.

doi:10.1101/624320

Cited by 3 publications

(3 citation statements)

References 59 publications

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“…Airway cells were isolated and cultured at an air‐liquid interface as previously described (Kuan et al, 2019; Reznikov et al, 2019). Cultures were treated with 100 nM SP (Sigma Aldrich) for two consecutive days to mimic the 48‐h exposure in lung slices.…”

Section: Methodsmentioning

confidence: 99%

Overexpression of Substance P in pig airways increases MUC5AC through an NF‐kβ pathway

et al. 2021

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Substance P (SP) is a tachykinin that regulates airway mucous secretion in both health and disease. Our study aimed to determine whether overexpression of SP without pre‐existing inflammation was sufficient to induce changes in mucin secretion and transport in small airways. Utilizing porcine precision‐cut lung slices, we measured the impact of AAV‐mediated overexpression of SP on airway physiology ex vivo. Immunofluorescence signal intensity for MUC5AC was significantly increased in SP‐overexpressed precision‐cut lung slices compared to GFP controls. No difference in MUC5B signal intensity between treatments was detected. SP‐overexpressed precision‐cut lung slices also exhibited decreased IL10 mRNA, an important inhibitor of mucous cell metaplasia. Overt deficits in mucociliary transport were not noted, though a trend for decreased mean transport speed was detected in methacholine‐challenged airways overexpressing SP compared to GFP controls. Pharmacologic inhibition of the NF‐kβ pathway abrogated the effects of overexpression of SP on both MUC5AC and IL10. Collectively, these data suggest that overexpression of SP in the absence of existing inflammation increases MUC5AC via activation of the NF‐kβ pathway. Thus, these data further highlight SP as a key driver of abnormal mucous secretion and underscore NF‐kβ signaling as a pathway of potential therapeutic intervention.

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Section: Methodsmentioning

confidence: 99%

Overexpression of Substance P in pig airways increases MUC5AC through an NF‐kβ pathway

et al. 2021

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“…MA512178), mouse anti‐acetyl α‐tubulin (clone 6‐11B‐1, 1:500; EMD Millipore, Burlington, MA, USA; catalogue no. MABT868) (Kuan et al., 2019), followed by goat anti‐rabbit and goat anti‐mouse secondary antibodies conjugated to Alexa‐Fluor 488 or 568 (1:1000; ThermoFisher Scientific, Waltham, MA, USA). We used WGA–rhodamine (Vector Laboratories) and jacalin–fluorescein isothiocyanate (Vector Laboratories, Burlingame, CA, USA) at 1:1000 dilution.…”

Section: Methodsmentioning

confidence: 99%

Airway cholinergic history modifies mucus secretion properties to subsequent cholinergic challenge in diminished chloride and bicarbonate conditions

Liao

Collins

Guevara

et al. 2020

Experimental Physiology

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Viral infections precipitate exacerbations in many airway diseases, including asthma and cystic fibrosis. Although viral infections increase cholinergic transmission, few studies have examined how cholinergic history modifies subsequent cholinergic responses in the airway. In our previous work, we found that airway resistance in response to a second cholinergic challenge was increased in young pigs with a history of airway cholinergic stimulation. Given that mucus secretion is regulated by the cholinergic nervous system and that abnormal airway mucus contributes to exacerbations of airway disease, we hypothesized that prior cholinergic challenge would also modify subsequent mucus responses to a secondary cholinergic challenge. Using our established cholinergic challenge-rechallenge model in pigs, we atomized the cholinergic agonist bethanechol or saline control to pig airways. Forty-eight hours later, we removed tracheas and measured mucus secretion properties in response to a second cholinergic stimulation. The second cholinergic stimulation was conducted in conditions of diminished chloride and bicarbonate transport to mimic a cystic fibrosis-like environment. In pigs previously challenged with bethanechol, a second cholinergic stimulation produced a mild increase in sheet-like mucus films; these films were scarcely observed in animals originally challenged with saline control. The subtle increase in mucus films was not associated with changes in mucociliary transport. These data suggest that prior cholinergic history might modify mucus secretion characteristics with subsequent stimulation in certain environmental conditions or disease states. Such modifications and/or more repetitive stimulation might lead to retention of mucus on the airway surface, thereby potentiating exacerbations of airway disease.

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“…Indeed, the rice rat has been used to study photoperiod and the pineal gland's role in reproduction and reproductive development Stetson, 1993, 1994;Edmonds et al, 1995;Edmonds and Stetson, 2001;Edmonds et al, 2005). They have also been utilized for studying the airway epithelium and disclose the distinctive transport properties that these species possess in their airways, which may explain their ability to survive in marsh salt environments (Kuan et al, 2019). The rice rat also appears to be a unique species for studying retinal pigmented epithelium degeneration (Casavant et al, 2000;Grahn et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Bone Structural, Biomechanical and Histomorphometric Characteristics of the Postcranial Skeleton of the Marsh Rice Rat (Oryzomys palustris)

Castillo

Croft

Jiron

et al. 2021

Preprint

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INTRODUCTION: The rice rat (Oryzomys palustris) is a non-conventional laboratory rodent species used to model some human bone disorders. However, no studies have been conducted to characterize the postcranial skeleton. Therefore, we aimed to investigate age- and gender-related features of the appendicular skeleton of this species. METHODS: We used femurs and tibiae from 94 rats of both genders aged 4-28 wks. Bone mineral content (BMC), bone mineral density (BMD), and biomechanical properties were determined in femurs. In addition, bone histomorphometry of tibiae was conducted to assess bone cells activities and bone turnover over time. RESULTS: Bodyweight, bone length, total metaphysis BMC/BMD, cortical BMC/BMD, cortical thickness, and cortical area progressively augmented with age. Whereas the increase in these parameters plateaued at age 16-22 wks in female rats, they continued to rise to age 28 wks in male rats. Furthermore, bone strength parameters increased with age, with few differences between genders. We also observed a rapid decrease in longitudinal growth between ages 4-16 wks. Whereas young rats had a greater bone formation rate and bone turnover, older rice rats had greater bone volume and trabecular thickness, with no differences between genders. CONCLUSIONS: 1) Sexual dimorphism in the rice rat becomes grossly evident at age 16 wks; 2) the age-related increases in bone mass, structural cortical parameters, and in some biomechanical property parameters plateau at an older age in male than in female rats; and 3) bone growth and remodeling significantly decreased with age indistinctive of the gender.

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Attenuated amiloride-sensitive current and augmented calcium-activated chloride current in marsh rice rat (Oryzomys palustris) airways

Cited by 3 publications

References 59 publications

Overexpression of Substance P in pig airways increases MUC5AC through an NF‐kβ pathway

Overexpression of Substance P in pig airways increases MUC5AC through an NF‐kβ pathway

Airway cholinergic history modifies mucus secretion properties to subsequent cholinergic challenge in diminished chloride and bicarbonate conditions

Bone Structural, Biomechanical and Histomorphometric Characteristics of the Postcranial Skeleton of the Marsh Rice Rat (Oryzomys palustris)

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