Attenuated alloreactivity of dendritic cells engineered with surface-modified microspheres carrying a plasmid encoding interleukin-10

Long, Jia; Kovacs, Jeffrey R.; Zheng, Ying; Gawalt, Ellen S.; Shen, Hongmei; Meng, Wilson S.

doi:10.1016/j.biomaterials.2005.09.032

Cited by 10 publications

(10 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2). In MS O10H6 , the DNA is adsorbed on the microsphere surface via ionic interactions and can be dislodged by heparin, an anionic polysaccharide [19,20]. In contrast, the DNA is embedded in PLGA O10H6 -ODN and cannot be released by surface displacement with heparin (data not shown).…”

Section: Discussionmentioning

confidence: 69%

See 1 more Smart Citation

Characterization of particles fabricated with poly(D,L-lactic-co-glycolic acid) and an ornithine–histidine peptide as carriers of oligodeoxynucleotide for delivery into primary dendritic cells

Zheng

Kovacs²,

Gawalt³

et al. 2006

Journal of Biomaterials Science, Polymer Edition

Self Cite

View full text Add to dashboard Cite

We report the formulation of particles to deliver oligodeoxynucleotides (ODN) into primary murine dendritic cells (DCs), the most potent antigen-presenting cells (APCs) known, using poly(D,L-lactic-co-glycolic acid) (PLGA) and a small cationic peptide. PLGA polymer and the ODN were fabricated into nano-sized spherical particles with the aid of O10H6 (O = ornithine, H = histidine). We have previously determined that O10H6 condenses DNA and is less toxic to DCs than a similar lysine-based peptide. The colloidal particles are stabilized by negative surface potentials. The peptide and the ODN can be detected in the fabricated particles with reflectance infrared spectroscopy. Significant ODN uptake can be observed in DCs exposed to the particles. Confocal imaging studies reveal that ODN can be internalized and escape from lysosomes in DCs. Taken together, these data suggest that combining PLGA and O10H6 is a feasible method to generate ODN-containing nano-sized particles for applications in DCs.

show abstract

Section: Discussionmentioning

confidence: 69%

“…We have previously shown that O10H6 forms stable condensates with nucleic acids on the surface of carboxylate polystyrene microspheres and this self-assembled system can facilitate DNA uptake in DCs [19,20]. The imidazole in histidine facilitates endosomal escape by disrupting the osmotic balance of the vesicles [21,22].…”

Section: Introductionmentioning

confidence: 99%

Characterization of particles fabricated with poly(D,L-lactic-co-glycolic acid) and an ornithine–histidine peptide as carriers of oligodeoxynucleotide for delivery into primary dendritic cells

Zheng

Kovacs²,

Gawalt³

et al. 2006

Journal of Biomaterials Science, Polymer Edition

Self Cite

View full text Add to dashboard Cite

show abstract

“…DNA co-internalized with O10H6 can escape endosomal sequestration [9], presumably assisted by the buffering capacity of the histidines [11,12]. DNA complexed with O10H6 is integrated into PLGA particles by adsorbtion to polyvinyl alcohol (PVA) on the surface [13,14]. Through hydrogen-bonding, O10H6 interacts with the alcohol groups of PVA, creating a cationic charge surface conducive for DNA binding.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Nickel-Decorated PLGA Particles Anchored with a His-tagged Polycation

Kovacs

Tidball

Ross

et al. 2009

Journal of Biomaterials Science, Polymer Edition

Self Cite

View full text Add to dashboard Cite

The pharmacological impact of oligodeoxynucleotides (ODN) as transcription factors decoys (anti-sense) depends on the efficiency of cellular uptake. In this study, we sought to generate nickel-decorated particles to facilitate the entry of ODN into dendritic cells (DCs), the primary instigators of immune responses. Nickel ions were incorporated into the matrix of poly(D,L-lactide-co-glycolide) (PLGA) particles using the metal chelating lipid DOGS-NTA-Ni. Submicrometer-sized PLGA particles containing nickel ions (PLGA-Ni) were formed using a double-emulsion solvent evaporation method. Infrared spectroscopy provided chemical proofs of nickel incorporation into the PLGA matrix. Binding of the polycation O10H6 raised the surface potential of PLGA-Ni from -17 mV to +13 mV. This change was partially reserved by the presence of free imidazole, suggesting the binding was mediated by nickel-histidine coordination. When compared to PLGA particles without nickel, ODN bound to O10H6-coated PLGA-Ni particles exhibited enhanced capacity to accumulate in DCs cultured in vitro. DCs exhibited cellular stress after exposure to PLGA-Ni complexed with O10H6 and DNA, but this effect can be prevented by serum and was reversed overnight. These data suggest PLGA-Ni should be further explored as a nucleic acid carrier in the context of anti-sense gene down-regulation.

show abstract

“…IL-10 can directly inhibit the phosphorylation of CD28, and thereby abrogates any further downstream signaling. In addition, when human naїve T cells were cultured with IL-10, with or without IFN-α, they became regulatory T cells that are able to suppress the activation and proliferation of other bystander T cells (8). IL-10 may also induce tolerance indirectly, as it interferes with the DC function.…”

Section: Introductionmentioning

confidence: 99%

IL-10 Gene Modified Dendritic Cells Inhibit T Helper Type 1-Mediated Alloimmune Responses and Promote Immunological Tolerance in Diabetes

et al. 2008

View full text Add to dashboard Cite

Attenuated alloreactivity of dendritic cells engineered with surface-modified microspheres carrying a plasmid encoding interleukin-10

Cited by 10 publications

References 30 publications

Characterization of particles fabricated with poly(D,L-lactic-co-glycolic acid) and an ornithine–histidine peptide as carriers of oligodeoxynucleotide for delivery into primary dendritic cells

Characterization of particles fabricated with poly(D,L-lactic-co-glycolic acid) and an ornithine–histidine peptide as carriers of oligodeoxynucleotide for delivery into primary dendritic cells

Characterization of Nickel-Decorated PLGA Particles Anchored with a His-tagged Polycation

IL-10 Gene Modified Dendritic Cells Inhibit T Helper Type 1-Mediated Alloimmune Responses and Promote Immunological Tolerance in Diabetes

Contact Info

Product

Resources

About