IN view of the rarity of induced epithelial tumours in fowls, it seems justifiable to record all successful attempts in this field. As described in earlier communications (Peacock andPeacock, 1949, 1954), we had repeatedly applied several well-known chemical carcinogens by various routes, mostly without success, though 2-acetylaminofluorene gave the most promising results. Subsequently we have observed another squamous carcinoma of the crop in a fowl injected intramurally with 23 mg. of 2-acetylaminofluorene given as a 1 per cent solution in arachis oil. Comparable intramural injections of methylcholanthrene in arachis oil have yielded 3 tumours, one of which has already been described (Peacock and Peacock, 1954).
METHODOf a 5 per cent solution (w/v) of methylcholanthrene in arachis oil 0*5 ml. was injected into the wall of the crop of 5 cocks and 4 hens of an inbred White Leghorn stock.Similar injections were repeated 3 times at intervals of 5 to 6 months; thereafter the surviving birds were injected more frequently at approximately monthly intervals, depending upon the palpability of the previous injections and the local reaction.The birds were kept on free grass range with access to unheated fowl houses and were fed twice daily with commercial poultry mash and pellets. Water was constantly available.
RESULTSThe birds that died or were killed within the first 30 months of the experiment showed no lesions attributable to the injections.The results are summarised in Table I, from which it will be seen that the first tumour was obtained after 35 months and that 2 of the 4 survivors died or were killed bearing epithelial tumours-one an adenocarcinoma of the proventriculus, the other a hepatoma.Post Mortem Findings Cock 3412 received 30 mg. methyleholanthrene in arachis oil and was killed 35 months after the start of the experiment. A tumour at the site of injection was described previously as an anaplastic carcinoma (Peacock and Peacock, 1954). Its histogenesis is still of a debatable nature (Fig. 1).