Attempts to determine the formation and character of γ-globulin and of natural and immune antibodies in young pigs reared without colostrum

Šterzl, J.; Kostka, J.; Říha, I.; Mandel, L.

doi:10.1007/bf02930894

Cited by 64 publications

(15 citation statements)

References 27 publications

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Section: Discussionmentioning

confidence: 92%

“…Sterzl et al (1960) estimated that the immunoglobulin content of pre-colostral piglet serum was 10-40 pg per ml, but were unable to demonstrate any antibody activity associated with this fraction. According to Sterzl et al (1964), the bactericidal effect of pre-colostral piglet serum depends on the amount of complement present. The bactericidal tests recorded by us were performed in the presence of excess of complement and it is therefore improbable that the results were influenced by differences in complement content of the original samples.…”

Section: Discussionmentioning

confidence: 92%

See 1 more Smart Citation

Transfer Of Escherichia Coli Antibodies From Sow To Piglet

Shreeve

Thomlinson

1971

Journal of Medical Microbiology

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EARLIER experiments Thomlinson, 1970a, 1971a) showed that piglets may be hypersensitive to certain serotypes of Escherichia coli at birth, and that pregnant sows immunised with either hen egg albumin or E. coli extracts conferred hypersensitivity on their young whether or not they had received colostrum. Sensitisation of the piglets may have resulted from transfer of maternal antibody across the placental barrier, or from transfer of antigen leading to active immunisation of the piglets.The purpose of the following experiments was to determine whether antibodies could be detected in the serum of newborn colostrum-deprived piglets from immunised dams and to investigate the origin of these antibodies. MATERIALS AND METHODSAnimals. Pregnant Large-White sows were obtained from the same source as those used in previous experiments (Shreeve and Thomlinson, 1970a). Piglets were separated from their dams before they received colostrum; blood samples were obtained from the anterior vena cava immediately after birth.Active immunisation ofsows. Five pregnant sows were immunised with E. coli serotype 0138:K81(B) extract prepared by the method of Erskine, Sojka and Lloyd (1957). The immunisation procedure was similar to that employed in previous experiments (Shreeve and Thomlinson, 1971a) except that, in two of the sows, it was begun in the first third of pregnancy, about the 35th day. All five received an intravenous injection of 2 ml of the same E. coli extract within 2 hr before parturition; this caused mild symptoms of shock. Two sows were immunised by the injection of a heat-killed formolised suspension of E. coli 0138:K81(B) by the intravenous route. Cultures were prepared on nutrient agar in Roux flasks and incubated at 37°C for 48 hr. The organisms were suspended and washed three times in sterile isotonic saline and were then heated in a waterbath at 60°C for 1 hr. Formaldehyde was added to give a concentration of 0.2 per cent. Before use, the suspension was diluted in saline to an opacity equal to that of Brown's tube no. 1. Three courses of injections were given at intervals of 10 days; each consisted of four injections at 48-hr intervals and the dose was increased progressively from 2 ml in the first course to 5 ml in the last. The final injection was given 10 days before the expected date of parturition.Passive immunisation of sows. Antiserum prepared from blood collected from sows that had been immunised with E. coli 0138:K81(B) extract was stored at -30°C. The pooled antiserum had an " 0 " agglutinin titre of 20,480. Two sows were each given an intravenous injection of 150 ml antiserum either 24 or 48 hr before parturition. This injection caused mild signs of shock.Immunoglobulin was prepared by adding ammonium sulphate to the antiserum to give a concentration of 40 per cent. The mixture was held at 4°C for 18 hr and centrifuged at 4500 r.p.m. for 45 min. The precipitate was dissolved in a minimal amount of distilled water and dialysed at 4°C overnight against a large volume of distilled water. The dialysate ...

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 92%

Transfer Of Escherichia Coli Antibodies From Sow To Piglet

Shreeve

Thomlinson

1971

Journal of Medical Microbiology

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“…Sterzl et al (18) have presented data which suggested to them that the gamma globulin in the pig at birth is not antibody. This concept would be supported by the experiences of Young and Underdahl who have used several thousand PFCD pigs in virus transmission experiments beginning in 1949.…”

Section: Immunity In Health-defined Animalsmentioning

confidence: 99%

Viral Infections of Domestic Animals

Young¹

1964

Annu. Rev. Microbiol.

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“…All serum proteins of foetal pigs are produced by the foetuses themselves and their immune response and, consequently, synthesis of immunoglobulins are therefore unaffected by the presence of maternal antibodies. For this reason, the foetal pig is an excellent model for studies of defence mechanisms in both human and veterinary medicine and has been receiving due attention as to the development of immunity.From a number of studies concerned with the incidence of immunoglobulins in foetal pigs and colostrum-deprived piglets (Sterzl et al 1960; Franek et al …”

mentioning

confidence: 99%

“…From a number of studies concerned with the incidence of immunoglobulins in foetal pigs and colostrum-deprived piglets (Sterzl et al 1960;Franek et al 1961;Sokol and i>urikovil! 1967;MenSik und Franz 1969;Proke §ova et a!.…”

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confidence: 99%

Synthesis of Immunoglobulins IgG, IgM and IgA during the Ontogeny of Foetal Pigs

Fränz¹,

Milon²,

Salmon³

1982

Acta Vet. Brno

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Transfer of proteins from dam to foetus has not been demonstrated in animals with the epitheliochorial placenta which include the pig (Sterzl and Silverstein 1967). Foetal pigs are therefore not affected by maternal immunoglobulins during their intra-uterine life. All serum proteins of foetal pigs are produced by the foetuses themselves and their immune response and, consequently, synthesis of immunoglobulins are therefore unaffected by the presence of maternal antibodies. For this reason, the foetal pig is an excellent model for studies of defence mechanisms in both human and veterinary medicine and has been receiving due attention as to the development of immunity.From a number of studies concerned with the incidence of immunoglobulins in foetal pigs and colostrum-deprived piglets (Sterzl et al. 1960; Franek et al.

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Attempts to determine the formation and character of γ-globulin and of natural and immune antibodies in young pigs reared without colostrum

Cited by 64 publications

References 27 publications

Transfer Of Escherichia Coli Antibodies From Sow To Piglet

Transfer Of Escherichia Coli Antibodies From Sow To Piglet

Viral Infections of Domestic Animals

Synthesis of Immunoglobulins IgG, IgM and IgA during the Ontogeny of Foetal Pigs

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