Attempts to Classify Herbicola Group-Enterobacter agglomerans Strains by Deoxyribonucleic Acid Hybridization and Phenotypic Tests

Brenner, Don J.; Fanning, G R; Knutson, Jean K. Leete; Steigerwalt, Arnold G.; Krichevsky, Micah I.

doi:10.1099/00207713-34-1-45

Cited by 53 publications

(49 citation statements)

References 22 publications

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“…o op pe en nU UP P ( (J Twenty-four Pantoea strains used in this study were previously used in DNA hybridisation studies [4], [5], [9], [10], [11] and [12] and are regarded as reference strains (Fig. 1).…”

Section: Faflp Analysis Of Pantoea Speciesmentioning

confidence: 99%

A FAFLP system for the improved identification of plant-pathogenic and plant-associated species of the genus Pantoea

Brady

Venter

Cleenwerck

et al. 2007

Systematic and Applied Microbiology

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Section: Faflp Analysis Of Pantoea Speciesmentioning

confidence: 99%

A FAFLP system for the improved identification of plant-pathogenic and plant-associated species of the genus Pantoea

Brady

Venter

Cleenwerck

et al. 2007

Systematic and Applied Microbiology

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“…Clustering between strains within a group was not identical in the two methods, but these fluctuations at the subgroup level are inconsequential as long as the aim is to ensure that strains remain within a species. Misidentified strains previously grouped into P. agglomerans were accurately assigned to P. ananatis, P. dispersa, or the recently defined species P. vagans or to discrete clades following Brenner's biogroups (10). For example, strains could be assigned to biogroup VII (LMG 5336, ATCC 27993, ATCC 27994, and EM2cb), biogroup VIII (LMG 5341, ATCC 27991, and ATCC 27992), and biogroup XII (LMG 5337, ATCC 27981, and ATCC 27990) using both MALDI-TOF MS and gyrB sequencing (Fig.…”

mentioning

confidence: 99%

Application of Whole-Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Rapid Identification and Clustering Analysis ofPantoeaSpecies

Rezzonico

Vogel²,

Duffy

et al. 2010

Appl Environ Microbiol

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Pantoea agglomerans is an ecologically diverse taxon that includes commercially important plant-beneficial strains and opportunistic clinical isolates. Standard biochemical identification methods in diagnostic laboratories were repeatedly shown to run into false-positive identifications of P. agglomerans, a fact which is also reflected by the high number of 16S rRNA gene sequences in public databases that are incorrectly assigned to this species. More reliable methods for rapid identification are required to ascertain the prevalence of this species in clinical samples and to evaluate the biosafety of beneficial isolates. Whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) methods and reference spectra (SuperSpectrum) were developed for accurate identification of P. agglomerans and related bacteria and used to detect differences in the protein profile within variants of the same strain, including a ribosomal point mutation conferring streptomycin resistance. MALDI-TOF MS-based clustering was shown to generally agree with classification based on gyrB sequencing, allowing rapid and reliable identification at the species level. (20) is a ubiquitous plant-epiphytic bacterium that belongs to the family Enterobacteriaceae. While several strains are commercialized for biological control of plant diseases (23), the species also includes two phytopathogenic pathovars that carry distinctive virulence plasmids (32). P. agglomerans has a Jekyll-Hyde nature, being described also as an opportunistic human pathogen (30), which raises biosafety regulatory issues for the utilization of beneficial isolates (45). Clinical reports predominantly involve septicemia following penetrating trauma (16, 56) or nosocomial infections (14,55). Clinical pathogenicity of this species has not been confidently confirmed (unfulfilled Koch's postulates). Infections attributed to P. agglomerans are typically of a polymicrobial nature involving patients affected by other diseases (14) and may represent secondary contamination of wounds. Standard clinical diagnostics and identification rely mainly on biochemical profiling analysis or alternatively on 16S rRNA gene sequencing, despite the inadequacy of these techniques for precise discrimination within the Enterobacter and Pantoea genera (5, 20, 39). Problems with correct identification have been observed for automated systems such as the API 20E (24, 39) and Vitek-2/GNIϩ (39, 40) (both from bioMerieux) or the Phoenix (11, 38) and Crystal identification systems (40, 48) (both from BD Diagnostic Systems). Pantoea agglomerans P. agglomerans is a composite taxon conglomerating formerEnterobacter agglomerans, Erwinia milletiae, and Erwinia herbicola strains. Accurate identification is complicated by the unsettled taxonomy of the "P. agglomerans-E. herbicola-E. agglomerans" complex (45). Recent analyses based on gyrB sequencing, multilocus sequence analysis (MLSA) (4), and fluorescent amplified fragment length polymorphisms (fAFLP) (45) indicate that strain...

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“…Interest in the "herbicola" group peaked in 1971, when such organisms were implicated in a nosocomial septicemia outbreak in the United States resulting from contaminated intravenous products in which 40 of 378 patients died (4,40). Ewing and Fife (19,20) compared the isolates from these patients with representatives of the "herbicola" group and concluded that the two sets of strains belonged to the same species.…”

mentioning

confidence: 99%

Subjective Synonymy of Erwinia herbicola, Erwinia milletiae, and Enterobacter agglomerans and Redefinition of the Taxon by Genotypic and Phenotypic Data

Amor

Mergaert

Gavini

et al. 1988

International Journal of Systematic Bacteriology

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The type strain Enterobacter agglomerans ATCC 27155 was examined for deoxyribonucleic acid (DNA) relatedness to 54 strains of the "Erwinia herbicola-Enterobacter agglomerans complex," to 23 strains of other Erwinia and Enterobacter species, and to 50 reference strains of 49 different species in other genera of the family Enterobacteriaceae. The DNA-DNA hybridization values (at the optimal renaturation temperature; nitrocellulose filter method) showed that 24 strains were highly related to Enterobacter agglomerans ATCC 2 7 W T (62 to 97% DNA relatedness) and formed a genotypic group provisionally called DNA hybridization group 27155. These strains were received as Enterobacter agglomerans (including strains of DNA hybridization groups V and Syst. Bacteriol. 34:45-55, 19841); as Erwinia herbicola, including the type strain NCPPB 2971; as Erwinia milletiae, including the type strain NCPPB 2519; and as yellow-pigmented Enterobacter strains. Numerical analysis of the protein electropherograms of these strains revealed the existence of seven protein electrophoretic groups, each showing a characteristic protein pattern. These seven groups separated at an infraspecific level and allowed assignment of 37 additional strains, received as Enterobacter agglomerans, Erwinia herbicola, and Erwinia milletiae, to DNA hybridization group 27155. The resulting group hybridized below 55% DNA relatedness with all remaining phenotypic or genotypic groups of the "Erwinia herbicola-Enterobacter agglomerans complex" and below 57% DNA relatedness with other Erwinia and Enterobacter species. DNA binding with 49 other species of the family Enterobacteriaceae was less than 38%. Since DNA hybridization group 27155 contains the type strains of Erwinia herbicola, Erwinia milletiae, and Enterobacter agglomerans, these species names are subjective synonyms, and the specific epithet agglomerans has priority. Further genotypic studies with several closely related genera are required for final placement of this species in a genus. The description of Enterobacter agglomerans, Erwinia herbicola, and Erwiniu milletiae is emended.

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Attempts to Classify Herbicola Group-Enterobacter agglomerans Strains by Deoxyribonucleic Acid Hybridization and Phenotypic Tests

Cited by 53 publications

References 22 publications

A FAFLP system for the improved identification of plant-pathogenic and plant-associated species of the genus Pantoea

A FAFLP system for the improved identification of plant-pathogenic and plant-associated species of the genus Pantoea

Application of Whole-Cell Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Rapid Identification and Clustering Analysis ofPantoeaSpecies

Subjective Synonymy of Erwinia herbicola, Erwinia milletiae, and Enterobacter agglomerans and Redefinition of the Taxon by Genotypic and Phenotypic Data

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