Attempts at grapevine (Vitis vinifera L.) breeding through genetic transformation: The main limiting factors

Saporta, R.; Pedro, Tania San; Gisbert, Carmina

doi:10.5073/vitis.2016.55.173-186

Cited by 8 publications

(7 citation statements)

References 29 publications

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“…Thus far, only two engineered viral vectors to induce pathogen confusion have been planned for the Apulian olive tree but results have not been reported yet [199]. Genetic engineering has not been widely used in Europe also due to the stronger negative perception of genetically modified organisms and its stricter regulation [200,201]. Additionally, the success of genetic transformation techniques has been limited by illegitimate recombination, non-mendelian inheritance of transgenes, availability of few characterized genes and the quantitative nature of some trait of interest [191,192,200].…”

Section: Genetic Engineeringmentioning

confidence: 99%

“…Genetic engineering has not been widely used in Europe also due to the stronger negative perception of genetically modified organisms and its stricter regulation [200,201]. Additionally, the success of genetic transformation techniques has been limited by illegitimate recombination, non-mendelian inheritance of transgenes, availability of few characterized genes and the quantitative nature of some trait of interest [191,192,200]. Caution must be taken when translating in vitro or in-house tests into expected patterns in the field, as recently shown by long-term studies in Xf [187].…”

Section: Genetic Engineeringmentioning

confidence: 99%

“…The advent of precision breeding technology or more efficient cis-genetic tools such as engineered site-specific endonucleases (e.g., CRISP/Cas9), which edit genomes without introducing foreign genes and create non-transgenic plants, could help overcome these issues but potential implications (e.g., cascade effects on trophic networks and/or ecosystem functioning, legitimacy to engineer wild species, etc.) should be carefully considered [191,192,[200][201][202][203].…”

Section: Genetic Engineeringmentioning

confidence: 99%

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From Nucleotides to Satellite Imagery: Approaches to Identify and Manage the Invasive Pathogen Xylella fastidiosa and Its Insect Vectors in Europe

et al. 2020

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Biological invasions represent some of the most severe threats to local communities and ecosystems. Among invasive species, the vector-borne pathogen Xylella fastidiosa is responsible for a wide variety of plant diseases and has profound environmental, social and economic impacts. Once restricted to the Americas, it has recently invaded Europe, where multiple dramatic outbreaks have highlighted critical challenges for its management. Here, we review the most recent advances on the identification, distribution and management of X. fastidiosa and its insect vectors in Europe through genetic and spatial ecology methodologies. We underline the most important theoretical and technological gaps that remain to be bridged. Challenges and future research directions are discussed in the light of improving our understanding of this invasive species, its vectors and host–pathogen interactions. We highlight the need of including different, complimentary outlooks in integrated frameworks to substantially improve our knowledge on invasive processes and optimize resources allocation. We provide an overview of genetic, spatial ecology and integrated approaches that will aid successful and sustainable management of one of the most dangerous threats to European agriculture and ecosystems.

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Section: Genetic Engineeringmentioning

confidence: 99%

Section: Genetic Engineeringmentioning

confidence: 99%

Section: Genetic Engineeringmentioning

confidence: 99%

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From Nucleotides to Satellite Imagery: Approaches to Identify and Manage the Invasive Pathogen Xylella fastidiosa and Its Insect Vectors in Europe

et al. 2020

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“…Proteomics studies performed on somatic embryogenesis processes have highlighted differences between embryogenic and nonembryogenic calli, allowing researchers to refine the culture conditions to increase the production of embryogenic tissues (Marsoni et al 2008;Correia et al 2016). Many successful reports have highlighted evidence that the genotype is one of the most relevant factors which influence initiation of the embryogenic process, and especially in Vitis vinifera, various embryogenic competencies have been observed in different cultivars (Gray 1995;Saporta et al 2016). One of the main aims of this study was to determine the most suitable and efficient strategy (explant and media compositions) for the induction of somatic embryogenesis in the grapevine cultivars Ancellotta, Lambrusco Salamino, Glera, and the rootstocks 110 Richter, 17.37, SO4, and Star 50.…”

Section: Discussionmentioning

confidence: 99%

From induction to embryo proliferation: improved somatic embryogenesis protocol in grapevine for Italian cultivars and hybrid Vitis rootstocks

Capriotti

Limera

Mezzetti

et al. 2022

Plant Cell Tiss Organ Cult

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Somatic embryogenesis is the most common regeneration method for the application of new genomic techniques like cisgenesis/intragenesis, genome editing, and RNAi. However, some local important genotypes show recalcitrance to this morphogenetic strategy, which represents an obstacle for the application of genetic engineering techniques. Whole flowers, stamens, and pistils of three different Italian Vitis vinifera L. cultivars (Ancellotta, Glera, and Lambrusco Salamino), and four hybrid rootstocks (110 Richter, 17.37, SO4, Star 50) have been tested in several culture media with changing basal salts (NN and MS), different combinations of growth regulators (BAP, 2,4-D, NOA, PIC, and NAA), and gelling agents, to initiate somatic embryogenesis. The formation of embryogenic calli was observed mainly from whole flowers cultured on PIV medium (NN salts, B5 vitamins, 3 g L−1 gelrite, 60 g L−1 sucrose, 8.9 µM BAP, and 4.5 µM 2,4-D), and stamens on MS1 medium (MS salts and vitamins, 7 g L−1 plant agar, 20 g L−1 sucrose, 4.5 µM BAP, and 5 µM 2,4-D), in the cv. Ancellotta, Lambrusco Salamino, and all the rootstocks, except for Star 50, which showed the best embryogenetic response from pistils placed on MS1. In a recalcitrant cv. as Glera, pistils placed on MS medium supplemented with 1 µM BAP, 5 µM 2,4-D, and gelrite as gelling agent, showed the highest percentage of embryogenesis. In addition, a two-step protocol was efficiently optimized for further induction of secondary embryo production for the above-listed grapevine genotypes, which guaranteed the long-term maintenance of embryogenic cultures from clusters or single somatic embryos.

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“…Therefore, transgenesis in grapevine is mostly based on the Agrobacterium system, and the regeneration of transformants is generally achieved with somatic embryogenesis. Improved Agrobacterium-mediated transformation protocols were published to enhance the fruit quality and tolerance to different stress factors [226,227].…”

Section: Genetic Transformationmentioning

confidence: 99%

Biotechnologies and Strategies for Grapevine Improvement

Butiuc-Keul

Coste

2023

Horticulturae

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Grapevine (Vitis vinifera subsp. Vinifera) is one of the most widespread and economically important perennial fruit crops in the world. Viticulture has changed over the years in response to changing environmental conditions and market demands, triggering the development of new and improved varieties to ensure the crop’s sustainability. The aim of this review is to provide a perspective on the recent developments in biotechnology and molecular biology and to establish the potential of these technologies for the genetic improvement of grapevine. The following aspects are discussed: (i) the importance of molecular marker-based methods for proper cultivar identification and how NGS-based high-throughput technologies have greatly benefited the development of genotyping techniques, trait mapping, and genomic selection; (ii) the recent advances in grapevine regeneration, genetic transformation, and genome editing, such as new breeding technology approaches for enhanced grapevine yield, quality improvement, and the selection of valuable varieties and cultivars. The specific problems and challenges linked to grapevine biotechnology, along with the importance of integrating classical and new technologies, are highlighted.

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Attempts at grapevine (Vitis vinifera L.) breeding through genetic transformation: The main limiting factors

Cited by 8 publications

References 29 publications

From Nucleotides to Satellite Imagery: Approaches to Identify and Manage the Invasive Pathogen Xylella fastidiosa and Its Insect Vectors in Europe

From Nucleotides to Satellite Imagery: Approaches to Identify and Manage the Invasive Pathogen Xylella fastidiosa and Its Insect Vectors in Europe

From induction to embryo proliferation: improved somatic embryogenesis protocol in grapevine for Italian cultivars and hybrid Vitis rootstocks

Biotechnologies and Strategies for Grapevine Improvement

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