Attainment and maintenance of pubertal cyclicity may predict reproductive longevity in beef heifers

Nafziger, Sarah R; Tenley, S. C.; Summers, Adam F.; Abedal-Majed, Mohamed A; Hart, Mariah; Bergman, Jeffrey W.; Kurz, Scott G.; Davis, John S.; Wood, Jennifer R.; Cupp, Andrea S.

doi:10.1093/biolre/ioab044

Cited by 12 publications

(14 citation statements)

References 39 publications

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“…Plasma progesterone concentrations were determined using a RIA to detect progesterone concentrations as previously described ( 76 ). Progesterone concentrations were determined using the ImmuChemTM Coated tube Progesterone 125I RIA kit (intra-assay CV = 5.64%, inter-assay CV=7.43%).…”

Section: Methodsmentioning

confidence: 99%

Prostaglandin F2α regulates mitochondrial dynamics and mitophagy in the bovine corpus luteum

et al. 2023

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Prostaglandins are arachidonic acid-derived lipid mediators involved in numerous physiological and pathological processes. PGF2α analogues are therapeutically used for regulating mammalian reproductive cycles and blood pressure, inducing term labor, and treating ocular disorders. PGF2α exerts effects via activation of calcium and PKC signaling, however, little is known about the cellular events imposed by PGF2α signaling. Here, we explored the early effects of PGF2α on mitochondrial dynamics and mitophagy in the bovine corpus luteum employing relevant and well characterized in vivo and in vitro approaches. We identified PKC/ERK and AMPK as critical protein kinases essential for activation of mitochondrial fission proteins, DRP1 and MFF. Furthermore, we report that PGF2α elicits increased intracellular reactive oxygen species and promotes receptor-mediated activation of PINK–Parkin mitophagy. These findings place the mitochondrium as a novel target in response to luteolytic mediator, PGF2α. Understanding intracellular processes occurring during early luteolysis may serve as a target for improving fertility.

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Section: Methodsmentioning

confidence: 99%

Prostaglandin F2α regulates mitochondrial dynamics and mitophagy in the bovine corpus luteum

et al. 2023

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“…These reproductive performance parameters indicate that heifers in the NC and SSD pubertal classifications would be predisposed to a reduced reproductive lifespan. Growth was also reduced in these two pubertal classifications with lower adjusted yearling weight when compared to E and T heifers (Nafziger et al, 2021). Within the UNL physiology herd, NC (delayed puberty) heifers comprise approximately 16% of the herd with 6% in the "false start" SSD group (Nafziger et al, 2021).…”

Section: Heritability Of Puberty Classificationsmentioning

confidence: 97%

Puberty classifications in beef heifers are moderately to highly heritable and associated with candidate genes related to cyclicity and timing of puberty

Hess,

Mersha,

Ference

et al. 2024

Front. Genet.

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Introduction: Pubertal attainment is critical to reproductive longevity in heifers. Previously, four heifer pubertal classifications were identified according to attainment of blood plasma progesterone concentrations > 1 ng/ml: 1) Early; 2) Typical; 3) Start-Stop; and 4) Non-Cycling. Early and Typical heifers initiated and maintained cyclicity, Start-Stop started and then stopped cyclicity and Non-Cycling never initiated cyclicity. Start-Stop heifers segregated into Start-Stop-Discontinuous (SSD) or Start-Stop-Start (SSS), with SSD having similar phenotypes to Non-Cycling and SSS to Typical heifers. We hypothesized that these pubertal classifications are heritable, and loci associated with pubertal classifications could be identified by genome wide association studies (GWAS).Methods: Heifers (n = 532; 2017 – 2022) genotyped on the Illumina Bovine SNP50 v2 or GGP Bovine 100K SNP panels were used for variant component estimation and GWAS. Heritability was estimated using a univariate Bayesian animal model.Results: When considering pubertal classifications: Early, Typical, SSS, SSD, and Non-Cycling, pubertal class was moderately heritable (0.38 ± 0.08). However, when heifers who initiated and maintained cyclicity were compared to those that did not cycle (Early+Typical vs. SSD+Non-Cycling) heritability was greater (0.59 ± 0.19). A GWAS did not identify single nucleotide polymorphisms (SNPs) significantly associated with pubertal classifications, indicating puberty is a polygenic trait. A candidate gene approach was used, which fitted SNPs within or nearby a set of 71 candidate genes previously associated with puberty, PCOS, cyclicity, regulation of hormone secretion, signal transduction, and methylation. Eight genes/regions were associated with pubertal classifications, and twenty-two genes/regions were associated with whether puberty was attained during the trial. Additionally, whole genome sequencing (WGS) data on 33 heifers were aligned to the reference genome (ARS-UCD1.2) to identify variants in FSHR, a gene critical to pubertal attainment. Fisher’s exact test determined if FSHR SNPs segregated by pubertal classification. Two FSHR SNPs that were not on the bovine SNP panel were selected for additional genotyping and analysis, and one was associated with pubertal classifications and whether they cycled during the trial.Discussion: In summary, these pubertal classifications are moderately to highly heritable and polygenic. Consequently, genomic tools to inform selection/management of replacement heifers would be useful if informed by SNPs associated with cyclicity and early pubertal attainment.

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“…Plasma progesterone concentrations were determined using a radioimmunoassay (RIA) to detect progesterone concentrations as previously described. 44 Progesterone concentrations were determined using the ImmuChemTM Coated Tube Progesterone 125I RIA kit (intra-assay CV = 2.0%, inter-assay CV = 4.46%). The sensitivity of the kit is 0.02 ng/mL.…”

Section: Progesterone Analysismentioning

confidence: 99%

Unraveling the role of lipid droplets and perilipin 2 in bovine luteal cells

Plewes,

Talbott,

Schott

et al. 2024

The FASEB Journal

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Steroidogenic tissues contain cytosolic lipid droplets that are important for steroidogenesis. Perilipin 2 (PLIN2), a structural coat protein located on the surface of lipid droplets in mammalian cells, plays a crucial role in regulating lipid droplet formation and contributing to various cellular processes such as lipid storage and energy homeostasis. Herein, we examine the role that PLIN2 plays in regulating progesterone synthesis in the bovine corpus luteum. Utilizing gene array databases and Western blotting, we have delineated the expression pattern of PLIN2 throughout the follicular to luteal transition. Our findings reveal the presence of PLIN2 in both ovarian follicular and steroidogenic luteal cells, demonstrating an increase in its levels as follicular cells transition into the luteal phase. Moreover, the depletion of PLIN2 via siRNA enhanced progesterone production in small luteal cells, whereas adenovirus‐mediated overexpression of both PLIN2 and Perilipin 3 (PLIN3) induced an increase in cytosolic lipid droplet accumulation and decreased hormone‐induced progesterone synthesis in these cells. Lastly, in vivo administration of the luteolytic hormone prostaglandin F2α resulted in an upregulation of PLIN2 mRNA and protein expression, accompanied by a decline in serum progesterone. Our findings highlight the pivotal role of PLIN2 in regulating progesterone synthesis in the bovine corpus luteum, as supported by its dynamic expression pattern during the follicular to luteal transition and its responsiveness to luteotropic and luteolytic hormones. We suggest PLIN2 as a potential therapeutic target for modulating luteal function.

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Attainment and maintenance of pubertal cyclicity may predict reproductive longevity in beef heifers

Cited by 12 publications

References 39 publications

Prostaglandin F2α regulates mitochondrial dynamics and mitophagy in the bovine corpus luteum

Prostaglandin F2α regulates mitochondrial dynamics and mitophagy in the bovine corpus luteum

Puberty classifications in beef heifers are moderately to highly heritable and associated with candidate genes related to cyclicity and timing of puberty

Unraveling the role of lipid droplets and perilipin 2 in bovine luteal cells

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