Attachment, re-mobilization, and inactivation of bacteriophage MS2 during bank filtration following simulation of a high virus load and an extreme rain event

Wang, He; Kaletta, Judith; Kaschuba, Sigrid; Klitzke, Sondra; Chorus, Ingrid; Griebler, Christian

doi:10.1016/j.jconhyd.2022.103960

Cited by 3 publications

(5 citation statements)

References 56 publications

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“…attachment to settling particles and sedimentation or transport in suspended fraction; Fauvel et al 2017 ); or (iv) during river bank filtration (e.g. straining or attachment in the aquifer or aquifer transport; Wang et al 2022 ). Mobility characteristics are often complex, as they are potentially influenced by physical, chemical and biological processes, depending on the aquatic scenario.…”

Section: Discussionmentioning

confidence: 99%

“…bacteriophages and enteric viruses) for groundwater transport studies is a relatively novel application of this technology, and therefore, limited literature exists. These approaches allow innovative analyses such as the quantification of multiple microorganism cotransport using multiplex qPCR and differentiating between infectious and inactivated viruses, when qPCR is used together with culture techniques (Betancourt et al 2014 , Bellou et al 2015 , Wang et al 2022 ). In addition, genetic methods are a reliable way to enumerate microorganisms attached to particles, such as sediment and microplastics (Hassard et al 2016 ).…”

Section: In-depth Review Of the Application Areas Of Genetic Faecal P...mentioning

confidence: 99%

“…Bauer et al ( 2011 ) used qPCR to analyse enteric adenoviruses to evaluate the efficiency of slow sand filtration and river bank filtration as drinking water treatment steps. Wang et al ( 2022 ) investigated the transport of MS2 phages, a surrogate for enteric viruses, from a surface water pond to groundwater via riverbank filtration. The authors differentiated between infectious phages by plaque assay versus the total number of phages detected by qPCR.…”

Section: In-depth Review Of the Application Areas Of Genetic Faecal P...mentioning

confidence: 99%

See 2 more Smart Citations

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Demeter

Linke

Reischer

et al. 2023

FEMS Microbiology Reviews

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The impacts on faecal pollution analysis using nucleic acid-based methods, such as PCR and sequencing, in health-related water quality research were assessed by rigorous literature analysis. A wide range of application areas and study designs has been identified since the first application more than 30 years ago (>1,100 publications). Given the consistency of methods and assessment types, we suggest defining this emerging part of science as a new discipline: genetic faecal pollution diagnostics (GFPD) in health-related microbial water quality analysis. Undoubtedly, GFPD has already revolutionised faecal pollution detection and microbial source tracking, the current core applications. GFPD is also expanding to many other research areas, including infection and health risk assessment, evaluation of microbial water treatment, and support of wastewater surveillance. In addition, storage of DNA extracts allows for biobanking, which opens up new perspectives. The tools of GFPD can be combined with cultivation-based standardised faecal indicator enumeration, pathogen detection, and various environmental data types, in an integrated data analysis approach. This comprehensive meta-analysis provides the scientific status quo of this field, including trend analyses and literature statistics, outlining identified application areas, and discussing the benefits and challenges of nucleic acid-based analysis in GFPD.

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Section: Discussionmentioning

confidence: 99%

Section: In-depth Review Of the Application Areas Of Genetic Faecal P...mentioning

confidence: 99%

Section: In-depth Review Of the Application Areas Of Genetic Faecal P...mentioning

confidence: 99%

See 1 more Smart Citation

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Demeter

Linke

Reischer

et al. 2023

FEMS Microbiology Reviews

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“…Apart from having different sedimentation rates, some indicators tend to attach to particles while others disperse freely in the water. In flowing watercourses, the latter tend to be transported more rapidly (Devane et al, 2022;Wang et al, 2022;Demeter et al, 2023).…”

Section: Biological Measuresmentioning

confidence: 99%

An overview of molecular markers for identification of non-human fecal pollution sources

Zlender,

Rupnik

2023

Front. Microbiol.

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Identifying primary sources of fecal pollution is important for assessing public health risks and implementing effective remediation strategies. To date, one of the main molecular approaches for identifying sources of fecal pollution relies on detecting molecular markers within bacterial, viral, or mitochondrial nucleic acids, that are indicative of a particular host. With a primary focus on identifying fecal pollution originating from humans, the field of fecal source tracking often places less emphasis on livestock sources, frequently leaving the problem of wildlife fecal pollution unaddressed. In this review, we summarize 55 previously published and validated molecular assays and describe methods for the detection of molecular markers that are indicative of non-human hosts. They cover a range of 15 animal species/groups with a primary focus on domestic animals including cattle, pigs, dogs, and poultry. Among assays associated with wild animals, the majority are designed to detect bird feces, while the availability of assays for detecting feces of other wild animals is limited. Both domestic and wild animals can represent a zoonotic reservoir of human enteropathogens, emphasizing the importance of their role in public health. This review highlights the need to address the complexity of fecal contamination and to include a broader range of animal species into assay validation and marker identification.

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“…Coliphages ΦX174 and MS2 were chosen as surrogates for the naturally occurring pathogenic enteric virus in many previous studies [22][23][24]. While they are not pathogenic, they are similar to a typical enteric virus in size.…”

Section: Bacteriophages and Plaque Assaymentioning

confidence: 99%

Fate and Transportation of Viruses from Reclaimed Water into a Floatation System

Dou

Kou

Sun

et al. 2022

Water

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The fate and transport of viruses in floatation systems is considerably important for accurate determination of the safety of reusing reclaimed water in the flotation process. Herein, simulation experiments on the floatation, adsorption and desorption were performed to examine the effect of initial virus concentration, pH and floatation reagents on the adsorption of viruses ΦΧ174 and MS2 onto copper–molybdenum ores. The transport of viruses in the flotation systems was also investigated. The viruses in the reclaimed water were rapidly adsorbed onto the ore particles, suggesting that tailing wastewater can be safely reused for floatation. However, the adsorbed viruses in the concentrates, middlings and tailings may pose health risks at certain exposure levels. The transport of viruses was dominated by their attachment to ore particles, with most being inactivated or irreversibly adsorbed. The removal and adsorption rates decreased as the initial virus concentration increased, and the removal rate decreased as pH was increased from 7.5 to 9.5. In comparison with MS2, ΦΧ174 was removed more effectively. This suggested that electrostatic repulsion is an important mechanism because MS2 has a greater negative charge. The attachment of both ΦΧ174 and MS2 onto the mineral particles increased significantly in the presence of PJ053 and CaO.

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Attachment, re-mobilization, and inactivation of bacteriophage MS2 during bank filtration following simulation of a high virus load and an extreme rain event

Cited by 3 publications

References 56 publications

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

Have genetic targets for faecal pollution diagnostics and source tracking revolutionized water quality analysis yet?

An overview of molecular markers for identification of non-human fecal pollution sources

Fate and Transportation of Viruses from Reclaimed Water into a Floatation System

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