Attachment of glycosaminoglycans to collagenous matrices modulates the tissue response in rats

Pieper, Jeroen; Wachem, P.B. van; Luyn, Marja J. A. van; Brouwer, Linda A.; Hafmans, T.G.M.; Veerkamp, J.H.; Kuppevelt, A.H.M.S.M. van

doi:10.1016/s0142-9612(00)00052-1

Cited by 145 publications

(106 citation statements)

References 33 publications

Supporting

Mentioning

103

Contrasting

Order By: Relevance

“…The biofunctionality and regenerative capacity of collagen can be significantly improved by the addition of glycosaminoglycans (GAGs) (Tierney et al, 2009). Scaffolds fabricated from copolymers of collagen and GAGs have been successfully applied in vitro and in vivo for skin, tendon, nerve, conjunctiva and more recently for bone and cartilage repair applications (Yannas and Burke, 1980, Louie et al, 1997, Chamberlain et al, 1998, Pieper et al, 2000, Hsu et al, 2000, Farrell et al, 2006, Lyons et al, 2010a.…”

Section: Introductionmentioning

confidence: 99%

“…Studies previously carried out 5 on cartilage regeneration have shown that GAGs are fundamental for presenting motifs for cell attachment and migration. In addition, GAGs have also been attributed to activating pathways that govern proliferation and differentiation as well as assembly of the ECM (Pieper et al, 2000, Banu and Tsuchiya, 2007, Wu et al, 2010, Correia et al, 2011. Specifically, chondroitin sulphate (CS) which is the main type of GAG in cartilage ECM has been shown to stimulate chondrogenesis in vitro and promotes cellular ingrowth and cartilaginous tissue formation in vivo (van Susante et al, 2001, Buma et al, 2003.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Addition of hyaluronic acid improves cellular infiltration and promotes early-stage chondrogenesis in a collagen-based scaffold for cartilage tissue engineering

Matsiko

Levingstone

O’Brien

et al. 2012

Journal of the Mechanical Behavior of Biomedical Materials

137

100

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Addition of hyaluronic acid improves cellular infiltration and promotes early-stage chondrogenesis in a collagen-based scaffold for cartilage tissue engineering

Matsiko

Levingstone

O’Brien

et al. 2012

Journal of the Mechanical Behavior of Biomedical Materials

137

100

View full text Add to dashboard Cite

“…Chondroitin sulfate is polyanionic and thus readily interacts with proteins in the ECM and binds effector molecules such as growth factors and cytokines that influence cell metabolism. [15][16][17] It has also been reported to promote chondrocyte adhesion via enhancing the attachment of chondronectin to collagen. 18 Previous studies have shown that the covalent attachment of CS to collagen matrices stimulated significantly higher chondrocyte proliferation and cartilage formation compared to collagen-only matrices both in vitro and in vivo.…”

Section: Introductionmentioning

confidence: 99%

Binding and Release Characteristics of Insulin-Like Growth Factor-1 from a Collagen–Glycosaminoglycan Scaffold

Mullen

Best

Brooks

et al. 2010

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

Tissue engineering is a promising technique for cartilage repair, but to optimize novel scaffolds before clinical trials, it is necessary to determine their characteristics for binding and release of growth factors. Toward this goal, a novel, porous collagen-glycosaminoglycan scaffold was loaded with a range of concentrations of insulinlike growth factor-1 (IGF-1) to evaluate its potential as a controlled delivery device. The kinetics of IGF-1 adsorption and release from the scaffold was demonstrated using radiolabeled IGF-1. Adsorption was rapid, and was approximately proportional to the loading concentration. Ionic bonding contributed to this interaction. IGF-1 release was studied over 14 days to compare the release profiles from different loading groups. Two distinct phases occurred: first, a burst release of up to 44% was noted within the first 24 h; then, a slow, sustained release (13%-16%) was observed from day 1 to 14. When the burst release was subtracted, the relative percentage of remaining IGF-1 released was similar for all loading groups and broadly followed t ½ kinetics until approximately day 6. Scaffold cross-linking using dehydrothermal treatment did not affect IGF-1 adsorption or release. Bioactivity of released IGF-1 was confirmed by seeding scaffolds (preadsorbed with unlabeled IGF-1) with human osteoarthritic chondrocytes and demonstrating increased proteoglycan production in vitro.

show abstract

“…The thickness of the capsule could be used as an indicator of the biocompatibility of the material: biomaterials with poor biocompatibility are generally covered with thicker capsules, which restrict angiogenesis and integration. 30 We observed an enhanced foreign body reaction for the COL-USPIO scaffolds (a thicker capsule in MRI): the capsule from subcutaneous COL-USPIO implants was always thicker than the capsule from COL implants. This indicates that USPIO incorporation leads to a more severe inflammatory response, but there were no signs of iron oxide-related toxicity and it is very unlikely that the scaffolds contain amounts beyond their safety level (2.6 mg Fe/kg).…”

Section: Fig 5 T 2 -Weighted Magnetic Resonance Imaging (Mri) Ofmentioning

confidence: 73%

In VivoMagnetic Resonance Imaging of Type I Collagen Scaffold in Rat: Improving Visualization of Bladder and Subcutaneous Implants

Sun

Geutjes²,

Oosterwijk³

et al. 2014

Tissue Engineering Part C: Methods

View full text Add to dashboard Cite

Noninvasive monitoring of implanted scaffolds is important to understand their behavior and role in tissue engineering, in particular to follow their degradation and interaction with host tissue. Magnetic resonance imaging (MRI) is well suited for this goal, but its application is often hampered by the low contrast of scaffolds that are prepared from biomaterials such as type I collagen. The aim of this study was to test iron oxide particles incorporation in improving their MRI contrasts, and to follow their degradation and tissue interactions. Scaffolds with and without iron oxide particles were implanted either subcutaneously or on the bladder of rats. At predetermined time points, in vivo MRI were obtained and tissues were then harvested for histology analysis and transmission electron microscopy. The result showed that the incorporation of iron oxide particles improved MRI contrast of the implants, providing information on their location, shapes, and degradation. Second, the host tissue reaction to the type I collagen implants could be observed in both MRI and histology. Finally, MRI also revealed that the degradation and host tissue reaction of iron particles-loaded scaffolds differed between subcutaneous and bladder implantation, which was substantiated by histology.

show abstract

Attachment of glycosaminoglycans to collagenous matrices modulates the tissue response in rats

Cited by 145 publications

References 33 publications

Addition of hyaluronic acid improves cellular infiltration and promotes early-stage chondrogenesis in a collagen-based scaffold for cartilage tissue engineering

Addition of hyaluronic acid improves cellular infiltration and promotes early-stage chondrogenesis in a collagen-based scaffold for cartilage tissue engineering

Binding and Release Characteristics of Insulin-Like Growth Factor-1 from a Collagen–Glycosaminoglycan Scaffold

In VivoMagnetic Resonance Imaging of Type I Collagen Scaffold in Rat: Improving Visualization of Bladder and Subcutaneous Implants

Contact Info

Product

Resources

About