Attachment of different Salmonella serovars to materials commonly used in a poultry processing plant

Chia, T. W. Raymond; Goulter, Rebecca M.; McMeekin, TA; Dykes, Gary A.; Fegan, Narelle

doi:10.1016/j.fm.2009.05.012

Cited by 139 publications

(99 citation statements)

References 38 publications

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“…Our finding that the genetic patterns of S. Corvallis recovered from different areas in the poultry processing plant were indistinguishable suggests that this strain is well adapted to the conditions at this plant. This adaptation may be attributed to the use of inadequate hygienic techniques [34] and/or to the ability of the bacteria to adhere to the materials used in the poultry industry [35]. In addition, strains of S. Corvallis with the same pulsotype presented different resistance patterns and ESBL production, suggesting that the mechanisms involved in these phenotypes are associated with mobile genetic elements, such as plasmids.…”

Section: Discussionmentioning

confidence: 99%

Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse

Yamatogi

Oliveira

Camargo

et al. 2015

J Infect Dev Ctries

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Introduction: Salmonella is a major cause of foodborne disease, and poultry products are important contributors to the transmission of this zoonotic pathogen. Although considered to be rare in most countries, Salmonella Corvallis has been reported in specific geographic areas isolated from both human and non-human sources. The aim of this study was to report the occurrence, the antimicrobial resistance profiles including the extended-spectrum beta-lactamase (ESBL) production, and the clonal relatedness of S. Corvallis strains. Methodology: A total of 132 fragments of poultry carcasses from a slaughterhouse in São Paulo State, Brazil, were collected at different stages of the manufacturing process (post-bleeding, post-plucking, and post-chilling) and analyzed for the presence of Salmonella. Antimicrobial resistance was determined by disc diffusion method and Etest. Clonal relatedness was determined by pulsed-field gel electrophoresis (PFGE). Results: Among the 272 Salmonella strains recovered, fourteen were S. Corvallis. Ten (71.4%) showed ESBL production and resistance to at least three antimicrobial agents. Nalidixic acid resistance and reduced ciprofloxacin susceptibility was verified in four (28.6%) strains. PFGE analyses showed that all the S. Corvallis strains belonged to the same pulsotype. Conclusion: This study identified genetically related S. Corvallis strains exhibiting ESBL production and reduced susceptibility to quinolone. The results suggest the need to improve the sanitary conditions in the slaughterhouse. Moreover, from a public health perspective, continuous surveillance on Salmonella is needed to control the dissemination of this important zoonotic pathogen and its resistance determinants.

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Section: Discussionmentioning

confidence: 99%

Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse

Yamatogi

Oliveira

Camargo

et al. 2015

J Infect Dev Ctries

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“…In several biofilm studies, the role of flagella in bacterial attachment has been investigated. In a comparison of the ability of several different Salmonella serovar strains to form biofilm in poultry-processing plants, it was concluded that the attachment of bacteria to different surfaces is strain dependent, and bacterial surface components, such as cell wall proteins, flagella, and lipopolysaccharide, all contribute to biofilm formation (20). Furthermore, transposon mutagenesis of S. Typhimurium in flagellar hook-associated protein and lipopolysaccharide synthesis severely altered attachment to meat and poultry and biofilm formation on different surfaces (21).…”

Section: Figmentioning

confidence: 99%

Salmonella enterica Serovar Kentucky Flagella Are Required for Broiler Skin Adhesion and Caco-2 Cell Invasion

Salehi

Howe

Lawrence

et al. 2017

Appl Environ Microbiol

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Nontyphoidal Salmonella strains are the main source of pathogenic bacterial contamination in the poultry industry. Recently, Salmonella enterica serovar Kentucky has been recognized as the most prominent serovar on carcasses in poultry-processing plants. Previous studies showed that flagella are one of the main factors that contribute to bacterial attachment to broiler skin. However, the precise role of flagella and the mechanism of attachment are unknown. There are two different flagellar subunits (fliC and fljB) expressed alternatively in Salmonella enterica serovars using phase variation. Here, by making deletions in genes encoding flagellar structural subunits (flgK, fliC, and fljB), and flagellar motor (motA), we were able to differentiate the role of flagella and their rotary motion in the colonization of broiler skin and cellular attachment. Utilizing a broiler skin assay, we demonstrated that the presence of FliC is necessary for attachment to broiler skin. Expression of the alternative flagellar subunit FljB enables Salmonella motility, but this subunit is unable to mediate tight attachment. Deletion of the flgK gene prevents proper flagellar assembly, making Salmonella significantly less adherent to broiler skin than the wild type. S. Kentucky with deletions in all three structural genes, fliC, fljB, and flgK, as well as a flagellar motor mutant (motA), exhibited less adhesion and invasion of Caco-2 cells, while an fljB mutant was as adherent and invasive as the wild-type strain.IMPORTANCE In this work, we answered clearly the role of flagella in S. Kentucky attachment to the chicken skin and Caco-2 cells. We demonstrated that the presence of FliC is necessary for attachment to broiler skin. Expression of the alternative flagellar subunit FljB enables Salmonella motility, but this subunit is unable to mediate strong attachment. Deletion of the flgK gene prevents proper flagellar assembly, making Salmonella significantly less adherent to broiler skin than the wild type. S. Kentucky with deletions in all three structural genes, fliC, fljB, and flgK, as well as a flagellar motor mutant (motA), exhibited less adhesion and invasion of Caco-2 cells, while an fljB mutant was as adherent and invasive as the wild-type strain. We expect these results will contribute to the understanding of the mechanisms of Salmonella attachment to food products.

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“…Stainless steel coupons were soaked in acetone for 30 min to remove grease and were rinsed in water before being soaked in 1 N NaOH for at least 1 h. The stainless steel coupons were subsequently rinsed in distilled water. All materials, glass slides, and stainless steel coupons were sterilized by autoclaving at 121 °C for 15 min (Chia et al, 2009). Stainless steel coupons were placed into 3.5 mL of LB wo /NaCl broth, and glass slides were placed into 4 mL of LB wo /NaCl broth media.…”

Section: Adhesion Assaysmentioning

confidence: 99%

The effect of marT gene on biofilm production of Salmonella Typhimurium

İlçe

Akçelik²

2015

Turk J Biol

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This study investigates the effect of MarT regulator protein on the production of Salmonella Typhimurium biofilm under in vitro culture conditions. The biofilm formation showed a statistically significant decrease compared to the wild-type strain at the two consecutive concentrations of arabinose (0.02% and 0.01%) in which the production of MarT protein was promoted to the highest level. When comparing the biofilm formation capacity to the wild type, the mutants containing a mutation in the genes investigated in terms of marT regulation showed statistically significant reductions, which were identified as fliA (59%), fliC (40%), fimA (46%), fidL (25%), and misL (25%) mutants. The biofilm structures of all strains used in this study were detected as the rdar morphotype in agar plates. Among the genes examined, fimA, slsA, fliA, dps, cheA, and wzzB genes were found to have a direct effect on the pellicle formation of S. Typhimurium. Finally, the biofilm formation features of the mutant strain, in which marT expression is induced, on glass, stainless steel, and polystyrene were also examined, and it was determined that the ability to produce biofilm decreased on all surfaces.

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Attachment of different Salmonella serovars to materials commonly used in a poultry processing plant

Cited by 139 publications

References 38 publications

Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse

Clonal relatedness and resistance patterns of Salmonella Corvallis from poultry carcasses in a Brazilian slaughterhouse

Salmonella enterica Serovar Kentucky Flagella Are Required for Broiler Skin Adhesion and Caco-2 Cell Invasion

The effect of marT gene on biofilm production of Salmonella Typhimurium

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