Attachment of Agrobacteria to Grape Cells

Pu, Xin-An; Goodman, R. N.

doi:10.1128/aem.59.8.2572-2577.1993

Cited by 10 publications

(8 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…, 2000) and A. vitis , the causal agent of crown gall in grapevine (Ophel & Kerr, 1990), attach to roots and are very good root colonizers (Brisset et al. , 1991; Pu & Goodman, 1993; Vicedo et al. , 1993; Peñalver et al.…”

Section: Introductionmentioning

confidence: 99%

Pathogenic and non‐pathogenic Agrobacterium tumefaciens, A. rhizogenes and A. vitis strains form biofilms on abiotic as well as on root surfaces

Abarca-Grau

Penyalver²,

López

et al. 2010

Plant Pathology

View full text Add to dashboard Cite

Pathogenic and non-pathogenic Agrobacterium tumefaciens, A. rhizogenes and A. vitis strains growing in minimal liquid medium adhered to different abiotic surfaces, forming biofilms at initial stages of development. Agrobacterium tumefaciens and A. vitis strains were able to attach to both polystyrene and polypropylene materials, whereas the A. rhizogenes strains only bound to polystyrene surfaces. Strains of the three species were also able to form biofilms on borosilicate coverslips. It is concluded that their ability to adhere to and form nascent biofilms on abiotic surfaces is dependent on the Agrobacterium species (biovar), surface material and growth conditions. Furthermore, tumorigenic A. tumefaciens and A. vitis strains, and the biological control agent A. rhizogenes strain K84, bound tightly to and formed complex biofilms on the surface of tomato root tips ex planta. More importantly, in planta assays confirmed that all three Agrobacterium spp. strains efficiently colonized tomato seedlings and also formed biofilms on roots. These complex structures, as revealed by scanning electron microscopy, were composed of numerous bacterial cells arranged in different ways: either dense and continuous carpets, large aggregates embedded in extra-cellular material or globular mushrooms traversed internally by channels. Confocal laser scanning microscopy, using GFP-marked derivative strains, corroborated the presence of live, three-dimensional and thick green fluorescent structures attached to plant material. This study illustrates that besides A. tumefaciens, strains of the species A. rhizogenes and A. vitis are also able to build biofilms on abiotic as well as on root surfaces.

show abstract

Section: Introductionmentioning

confidence: 99%

Pathogenic and non‐pathogenic Agrobacterium tumefaciens, A. rhizogenes and A. vitis strains form biofilms on abiotic as well as on root surfaces

Abarca-Grau

Penyalver²,

López

et al. 2010

Plant Pathology

View full text Add to dashboard Cite

show abstract

“…A more likely reason is that pAtK84b carries genes which are important in early stages of the pathogenic process, e.g., attachment and the potential for competition with or inhibition of pathogens. This possibility is supported by studies which have shown that nopaline-type Ti plasmids enhance attachment (23,29). Alternatively, pAtK84b may enhance chemotaxis to wound sites, as Ti plasmids have been shown to play a role in chemotaxis to wound exudates (1,2).…”

Section: Discussionmentioning

confidence: 93%

Construction of a Range of Derivatives of the Biological Control Strain Agrobacterium rhizogenes K84: a Study of Factors Involved in Biological Control of Crown Gall Disease

McClure

Ahmadi

Clare

1998

Appl Environ Microbiol

View full text Add to dashboard Cite

The biological control strain Agrobacterium rhizogenesK84 is an effective agent in the control of Agrobacteriumpathogens, the causative agents of crown gall disease. A number of factors are thought to play a role in the control process, including production of the specific agrocins 84 and 434, which differ in the spectra of pathogenic strains that they inhibit in vitro. A range of derivatives of strain K84 has been developed with every combination of the three resident plasmids, pAgK84, pAgK434, and pAtK84b, including a plasmid-free strain. These derivatives produced either both, one, or neither of the characterized agrocins 84 and 434 and were isolated by plasmid curing, conjugation, and Tn5 transposon mutagenesis. The ability of the derivative strains to inhibit gall formation on almond roots was compared to that of the wild-type K84 parent. Treatment with the plasmid-free derivative did not result in a significant level of control of an A. rhizogenes pathogen based on numbers or dry weight of galls formed on injured almond roots. The presence of plasmid pAgK84, pAgK434, or pAtK84b significantly enhanced the biological control efficacy of K84 derivatives, and the highest level of control was observed with strains harboring two or more plasmids. The results observed with strains deficient in agrocin 434 production suggest that this product may play an important role in the biological control of A. rhizogenes pathogens. The involvement of plasmid pAgK84b in biological control has not previously been reported. This study supports the conclusion that multiple factors are involved in the success of strain K84 as a biological control agent.

show abstract

“…It is known that a variety of chromosomal genes (chvA, chvB, pscA, and cel genes) are required for attachment of A. tumefaciens to various plant species (Matthysse & McMahan, 1998). However, there is evidence that the Ti plasmid can also contribute to attachment (Pu & Goodman, 1993). It has been previously demonstrated that A. vitis attaches more efficiently than A. tumefaciens to grapevine roots and that pehA, coding for polygalacturonase production by A. vitis, may be associated with attachment (Brisset et al, 1991;Rodriguez-Palenzuela et al, 1991).…”

Section: Discussionmentioning

confidence: 99%

Mutations affecting chemotaxis of Agrobacterium vitis strain E26 also alter attachment to grapevine roots and biocontrol of crown gall disease

Yang

Wang

et al. 2009

Plant Pathology

View full text Add to dashboard Cite

Crown gall in grapevine, caused by tumorigenic Agrobacterium vitis strains, can cause severe losses in most viticulture regions worldwide. The only effective means of control is through cultivation practices. One non-tumorigenic A. vitis strain, E26, can prevent crown gall infection when applied to wounds on grapevine prior to or simultaneous with inoculation of tumorigenic strains. ME19, a Tn5 mutant of strain E26, was impaired in terms of its ability to be chemoattracted by grapevine root tissue extracts and its attachment to grapevine roots, and had reduced biological control activity; it did not significantly differ from the wild-type strain of E26 in phenotypes of agrocin production, growth in minimum medium or swarming activity. Complementation of ME19 with the cosmid clone of CP1543 from an E26 DNA library restored the chemotaxis, attachment, and biocontrol phenotypes. A 7·3-kb Kpn I fragment from CP1543 was cloned and sequenced, and sequence analysis revealed that the Tn 5 insertion occurred in a region that shares a significant homology with genes coding for methyl-accepting chemotaxis proteins (MCPs) in many bacteria. Complementation of the mcp gene mutants restored the affected phenotypes to the level of wild-type E26. An in-frame deletion mutant of the mcp gene was generated and was determined to have the same phenotypes as the original Tn5 mutant.

show abstract

Attachment of Agrobacteria to Grape Cells

Cited by 10 publications

References 34 publications

Pathogenic and non‐pathogenic Agrobacterium tumefaciens, A. rhizogenes and A. vitis strains form biofilms on abiotic as well as on root surfaces

Pathogenic and non‐pathogenic Agrobacterium tumefaciens, A. rhizogenes and A. vitis strains form biofilms on abiotic as well as on root surfaces

Construction of a Range of Derivatives of the Biological Control Strain Agrobacterium rhizogenes K84: a Study of Factors Involved in Biological Control of Crown Gall Disease

Mutations affecting chemotaxis of Agrobacterium vitis strain E26 also alter attachment to grapevine roots and biocontrol of crown gall disease

Contact Info

Product

Resources

About