Attachment, morphology, and protein expression of rat marrow stromal cells cultured on charged substrate surfaces

Qiu, Qingqing; Sayer, M.; Kawaja, Michael D.; Shen, Xin; Davies, John E.

doi:10.1002/(sici)1097-4636(199810)42:1<117::aid-jbm15>3.0.co;2-i

Cited by 94 publications

(56 citation statements)

References 33 publications

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“…Therefore, the interaction of biological molecules, especially proteins, with biomaterials has been studied extensively. Much work has been done to investigate the influence of surface properties, such as charge and hydrophobicity, on the adsorption of proteins and subsequently, the responses of cells [8,19,20,[28][29][30][31][32][33]. However, the results have been confusing (for a recent review, see Chapter 12 of [34]).…”

Section: Discussionmentioning

confidence: 99%

“…At physiological conditions, pH=7.4, anionic gels are negatively charged and the electrostatic repulsion among charges on the polymer chain result in significant swelling [2,14]. Anionic hydrogels in highly swollen state should be able to trap a large amount of proteins and other bioactive molecules in biological fluids, either by protein entanglement in the surface or electrostatic interactions [18][19][20]. We speculated that this phenomenon, if it occurs, might provide essential molecular interaction sites for gels to support cellular activities.…”

Section: Introductionmentioning

confidence: 99%

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Improved cell adhesion and proliferation on synthetic phosphonic acid-containing hydrogels

et al. 2005

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improved cell adhesion and proliferation on synthetic phosphonic acid-containing hydrogels

et al. 2005

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“…The importance of surface charges and its influence on cell attachment and function has been emphasized by other workers. 29 From this perspective, the observation that a Ti surface grafted with peptide exhibited a limited decrease in hydrophobicity supports the hypothesis that chemical modification minimally influences cell attachment and function.…”

Section: Discussionmentioning

confidence: 99%

RGDS peptides immobilized on titanium alloy stimulate bone cell attachment, differentiation and confer resistance to apoptosis

Secchi

Grigoriou

Shapiro³

et al. 2007

J Biomedical Materials Res

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. (2007). RGDS peptides immobilized on titanium alloy stimulate bone cell attachment, differentiation and confer resistance to apoptosis. Retrieved from http://repository.upenn.edu/be_papers/101 RGDS peptides immobilized on titanium alloy stimulate bone cell attachment, differentiation and confer resistance to apoptosis Abstract A major cause of implant failure in skeletal tissues is failure of osseointegration, often due to lack of adhesion of cells to the titanium (Ti) alloy interface. Since arginine-glycine-aspartic acid (RGD)-containing peptides have been shown to regulate osteoblast adhesion, we tested the hypothesis that, bound to a Ti surface, these peptides would promote osteoblasts differentiation, while at the same time inhibit apoptosis. RGDS and RGES (control) peptides were covalently linked to Ti discs using an APTS linker. While the grafting of both RGDS and RGES significantly increased Ti surface roughness, contact angle analysis showed that APTS significantly increased the surface hydrophobicity; when the peptides were tethered to Ti, this was reduced. To evaluate attachment, MC3T3-E1 osteoblast cells were grown on these discs. Significantly more cells attached to the Ti-grafted RGDS then the Ti-grafted RGES control. Furthermore, expression of the osteoblasts phenotype was significantly enhanced on the Ti-grafted RGDS surface. When cells attached to the Ti-grafted RGDS were challenged with staurosporine, an apoptogen, there was significant inhibition of apoptosis; in contrast, osteoblasts adherent to the Ti-grafted RGES were killed. It is concluded that RGDcontaining peptides covalently bonded to Ti promotes osteoblasts attachment and survival with minimal changes to the surface of the alloy. Therefore, such modifications to Ti would have the potential to promote osseointegration in vivo. Abstract: A major cause of implant failure in skeletal tissues is failure of osseointegration, often due to lack of adhesion of cells to the titanium (Ti) alloy interface. Since arginine-glycine-aspartic acid (RGD)-containing peptides have been shown to regulate osteoblast adhesion, we tested the hypothesis that, bound to a Ti surface, these peptides would promote osteoblasts differentiation, while at the same time inhibit apoptosis. RGDS and RGES (control) peptides were covalently linked to Ti discs using an APTS linker. While the grafting of both RGDS and RGES significantly increased Ti surface roughness, contact angle analysis showed that APTS significantly increased the surface hydrophobicity; when the peptides were tethered to Ti, this was reduced. To evaluate attachment, MC3T3-E1 osteoblast cells were grown on these discs. Significantly more cells attached to the Ti-grafted RGDS then the Ti-grafted RGES control. Furthermore, expression of the osteoblasts phenotype was significantly enhanced on the Ti-grafted RGDS surface. When cells attached to the Ti-grafted RGDS were challenged with staurosporine, an apoptogen, there was significant inhibition of apoptosis; in contrast, osteoblasts adherent to the Ti-gra...

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“…[8][9][10][11] A number of studies have focused on how cell adhesion and shape are affected by chemically charged substrates with corresponding functional chemical groups such as -COOH (negatively charged at pH 7.4) and -NH 2 (positively charged at pH 7.4). 9,[12][13][14][15] Few studies have investigated the effects of electric charge 12 or charged nanoparticles 13,14 on cell adhesion and shape. It is widely accepted that negative and positive charges influence cell behavior, however, it remains unclear whether cell adhesion and shape are affected by charge alone 8,[11][12][13][14][15][16][17][18] because it is difficult to separate charge effects on cell adhesion and shape.…”

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confidence: 99%

Charged Nanomatrices as Efficient Platforms for Modulating Cell Adhesion and Shape

Kim

Lim

et al. 2012

Tissue Engineering Part C: Methods

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In this article, we describe the design and manipulation of charged nanomatrices and their application as efficient platforms for modulating cell behaviors. Using electrospraying technology and well designed biomaterials, poly(e-caprolactone; PCL) and polyethylenimine, the negatively charged PCL nanomatrix (nPCL nanomatrix) and the positively charged PCL nanomatrix (pPCL nanomatrix) were fabricated. It was demonstrated that cell adhesion, affinity, and shape were sensitively modulated in negatively and positively charged nanomatrices. Our results showed that the pPCL nanomatrix promoted adhesion of NIH 3T3 fibroblast cells as compared to the nPCL nanomatrix. When fluid shear stress was applied, cell affinity on the pPCL nanomatrix increased even more. NIH 3T3 fibroblast cells adopted a relatively spherical shape on the pPCL nanomatrix while adopting an aligned, narrow shape on the nPCL nanomatrix. It was also found that charged nanomatrices influenced the cross-sectional cell shape. The cross-sectional cell shape on the pPCL nanomatrix was extremely flattened, whereas the cross-sectional cell shape was relatively round on the nPCL nanomatrix and some of the adhered cells floated. We also showed that the surfaces of the nPCL and pPCL nanomatrices adsorbed the different serum proteins. These results collectively demonstrated a combination of environmental factors including nanoscale structure, electrostatic forces, and absorption of biomolecules on charged substrates affected cell response in terms of cellular adhesion and shape.

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Attachment, morphology, and protein expression of rat marrow stromal cells cultured on charged substrate surfaces

Cited by 94 publications

References 33 publications

Improved cell adhesion and proliferation on synthetic phosphonic acid-containing hydrogels

Improved cell adhesion and proliferation on synthetic phosphonic acid-containing hydrogels

RGDS peptides immobilized on titanium alloy stimulate bone cell attachment, differentiation and confer resistance to apoptosis

Charged Nanomatrices as Efficient Platforms for Modulating Cell Adhesion and Shape

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