Attachment and growth of human bone marrow derived mesenchymal stem cells on regenerated antheraea pernyi silk fibroin films

Luan, Xiying; Wang, Yong; Ding, Xuanming; Duan, Qiao-yan; Li, Mingzhong; Lu, Shenzhou; Zhang, Huanxiang; Zhang, Xueguang

doi:10.1088/1748-6041/1/4/001

Cited by 49 publications

(36 citation statements)

References 43 publications

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“…Compared with B. mori silk fibroin, the results showed that Ap-SF readily allowed cell attachment and growth. By studying the attachment, morphology, growth and phenotype of human bone marrow derived mesenchymal stem cells (hBMSCs) cultured on Ap-SF films, it was found that Ap-SF, such as B. mori silk fibroin and collagen, could support hBMSC attachment, growth and phenotypic maintenance, and has better biocompatibilities for in vitro culture of hBMSCs [12]. Based on these features, interest has risen in the use of Ap-SF as a starting material for advanced biomedical applications such as cell culture substrates, surgical mending materials and tissue engineering scaffolds [13][14][15][16][17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Gelation behavior of Antheraea pernyi silk fibroin

Yan

Zhao

et al. 2010

Sci. China Chem.

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The sol-gel transition behavior of Antherae pernyi silk fibroin (Ap-SF) has not been systematically investigated. In this work, the influence of environmental temperature, pH, the concentration of Ap-SF, K + and Ca 2+ on the gelation time, and the structural changes of Ap-SF in sol-gel transformation were studied. The results indicated that the gelation time of the Ap-SF aqueous solution decreased with the increase of the Ap-SF concentration and environmental temperature. The sol-gel transformation of Ap-SF was much more rapid than that of Bombyx mori silk fibroin under the same conditions. The Ap-SF was sensitive to changes in the concentration of Ca 2+ and K + . Upon gelation, the random coil structure of the Ap-SF was significantly transformed into the -sheet structure.

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Section: Introductionmentioning

confidence: 99%

Gelation behavior of Antheraea pernyi silk fibroin

Yan

Zhao

et al. 2010

Sci. China Chem.

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“…35 Similarly, non-mulberry silk fibroin-based materials from the Samia ricini and Antharae pernyi silkworms have supported fibroblast-like, osteoblast-like, and bone marrow-derived mesenchymal stem cell growth in vitro. 42,43 In this study, we demonstrated the cytocompatibility of Spidrex, showing that primary human and rat tenocyte viability increased over 14 days of culture on Spidrex. Morphological analysis of calcein-AM-stained human tenocytes cultured on the different silk scaffolds and within collagen gels demonstrated that human tenocytes retained their native elongated morphology when cultured on Spidrex, whereas human tenocytes cultured on the knitted B. mori scaffold and within the collagen gels did not appear as tenocytic.…”

Section: Figmentioning

confidence: 55%

“…[32][33][34] Studies evaluating silk as a biomaterial for tendon regeneration have largely focused on the mulberry B. mori silk or composites of B. mori silk with either synthetic polymers or collagen. 28,29,[35][36][37][38] However, given that non-mulberry silks contain the cell binding RGD tripeptide motif, [39][40][41] and have been shown to support fibroblast-like and bone marrow-derived mesenchymal stem cell growth in vitro, 42,43 non-mulberry silk-derived scaffolds hold much promise as biomaterials for enhancing tendon tissue regeneration. 44 In this study, in vitro assays were used to assess the cytocompatibility and immunogenicity of a novel knitted, nonmulberry silk fibroin scaffold designed for use in tendon tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

In VitroEvaluation of a Novel Non-Mulberry Silk Scaffold for Use in Tendon Regeneration

Musson

Naot

Chhana

et al. 2015

Tissue Engineering Part A

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Tearing of the rotator cuff tendon in the shoulder is a significant clinical problem, with large/full-thickness tears present in *22% of the general population and recurrent tear rates postarthroscopic repair being quoted as high as 94%. Tissue-engineered biomaterials are increasingly being investigated as a means to augment rotator cuff repairs, with the aim of inducing host cell responses to increase tendon tissue regeneration. Silk-derived materials are of particular interest due to the high availability, mechanical strength, and biocompatibility of silks. In this study, Spidrex Ò , a novel knitted, non-mulberry silk fibroin scaffold was evaluated in vitro for its potential to improve tendon regeneration. Spidrex was compared with a knitted Bombyx mori silk scaffold, a 3D collagen gel and Fiberwire Ò suture material. Primary human and rat tenocytes successfully adhered to Spidrex and significantly increased in number over a 14 day period ( p < 0.05), as demonstrated by fluorescent calcein-AM staining and alamarBlue Ò assays. A similar growth pattern was observed with human tenocytes cultured on the B. mori scaffold. Morphologically, human tenocytes elongated along the silk fibers of Spidrex, assuming a tenocytic cell shape, and were less circular with a higher aspect ratio compared with human tenocytes cultured on the B. mori silk scaffold and within the collagen gel ( p < 0.05). Gene expression analysis by real-time PCR showed that rat tenocytes cultured on Spidrex had increased expression of tenocyte-related genes such as fibromodullin, scleraxis, and tenomodulin ( p < 0.05). Expression of genes that indicate transdifferentiation toward a chondrocytic or osteoblastic lineage were significantly lower in tenocytes cultured on Spidrex in comparison to the collagen gel ( p < 0.05). Immunogenicity assessment by the maturation of and cytokine release from primary human dendritic cells demonstrated that Spidrex enhanced dendritic cell maturation in a similar manner to the clinically used suture material Fiberwire, and significantly upregulated the release of proinflammatory cytokines ( p < 0.05). This suggests that Spidrex may induce an early immune response postimplantation. While further work is required to determine what effect this immune response has on the tendon healing process, our in vitro data suggests that Spidrex may have the cytocompatibility and bioactivity required to support tendon regeneration in vivo.

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“…From its commercial use, decades ago, as a biomedical suture, to today's tissue engineering scaffolds, SF has been studied as an ideal biomaterial for a range of applications, including tissue repair and induction of tissue regeneration. Many studies have revealed that silkworm SF, such as B mori SF in various shapes (such as fibers, films, nets, mats, and sponges), can support the adhesion and proliferation of many kinds of cells, including keratinocytes, fibroblasts, vascular endothelial cells, epithelial cells, bone marrow stromal cells and neuroglia cells [1][2][3][4][5][6][7][8] . In particular, stem cellbased tissue engineering with B mori SF has expanded the use of silk-based biomaterials for bone, ligament, cartilage and connective tissues [1][2][3][4] .…”

Section: Introductionmentioning

confidence: 99%

“…The SF from the wild silkworm A pernyi or A mylitta has attracted much attention as biomaterials due to a functional Arg-Gly-Asp (RGD) tripeptide sequence [7,8] . The RGD tri-peptide, found within fibronectin, laminin, vitronectin, collagen and other related adhesion molecules in extracellular matrix (ECM) proteins, has been discovered to be a recognition site for adhesion, spreading and motility of cells [9,10] .…”

Section: Introductionmentioning

confidence: 99%

Preliminary biocompatibility evaluation of regenerated Antheraea yamamai Silk Fibroin in vitro

Wang

Huiuk

Wei

2011

J. Wuhan Univ. Technol.-Mat. Sci. Edit.

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The study aimed to investigate the adhesion, morphology and proliferation of Sprague Dawley (SD) albino rat bone marrow-derived mesenchymal stem cells (BMSCs) by inverted microscope, cell counting, MTT test and laser scanning confocal microscop (LSCM). On the regenerated A yamamai SF film or blend fi lms, the cell morphology was almost the same as that on collagen (collagen type I) fi lm, the cell adhesion rate was higher than that on plastic cell plate and B mori SF fi lm after 1 h (p < 0.01) of culture, and the cell proliferation was signifi cantly higher than that on collagen fi lm (p < 0.01) and plastic cell plate (p < 0.01), and also obviously better than that on B mori SF fi lm. On the other hand, the viability of BMSCs in the regenerated A yamamai SF porous scaffold was better than that in B mori SF porous scaffold and medical grade polyvinyl alcohol (PVA) sponge.

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Attachment and growth of human bone marrow derived mesenchymal stem cells on regenerated antheraea pernyi silk fibroin films

Cited by 49 publications

References 43 publications

Gelation behavior of Antheraea pernyi silk fibroin

Gelation behavior of Antheraea pernyi silk fibroin

In VitroEvaluation of a Novel Non-Mulberry Silk Scaffold for Use in Tendon Regeneration

Preliminary biocompatibility evaluation of regenerated Antheraea yamamai Silk Fibroin in vitro

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