Attaching and effacing Escherichia coli isolated from dogs in Brazil: characteristics and serotypic relationship to human enteropathogenic E. coli (EPEC)

Nakazato, Gerson; Gyles, Carlton; Ziebell, Kim; Keller, Rogéria; Trabulsi, Luíz Rachid; Gomes, Tânia A. T.; Irino, Kinue; Silveira, Wanderley Dias da; Castro, Antonio Fernando Pestana de

doi:10.1016/j.vetmic.2004.04.009

Cited by 53 publications

(74 citation statements)

References 32 publications

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“…On the contrary, Zahraei Salehi et al (Zahraei Salehi et al 2011) reported that 6 (6%) isolates among 10 pathogenic E. coli isolates from dogs without diarrhoea in Iran were EPEC strains. The 50% eae gene (combined with stx gene) prevalence noted in this study is higher when compared with 13, 17.6 and 20% eae gene prevalence among 19, 12 and 34 E. coli isolates from 146, 68 and 51 dogs with diarrhoea reported in Canada (Nakazato et al 2004), Brazil (Puno-Sarmiento et al 2013) and Egypt (Ali and Metwaly 2015), respectively. It is also higher than 8 and 10.5% eae gene prevalence among 36 and 86 E. coli isolates from dogs without diarrhoea reported in Canada (Nakazato et al 2004) and Brazil (Puno-Sarmiento et al 2013), respectively.…”

Section: 4% a A D ( A 1 ) T E T ( A ) T E T ( B ) D F R ( A 1 ) S contrasting

confidence: 43%

“…The 50% eae gene (combined with stx gene) prevalence noted in this study is higher when compared with 13, 17.6 and 20% eae gene prevalence among 19, 12 and 34 E. coli isolates from 146, 68 and 51 dogs with diarrhoea reported in Canada (Nakazato et al 2004), Brazil (Puno-Sarmiento et al 2013) and Egypt (Ali and Metwaly 2015), respectively. It is also higher than 8 and 10.5% eae gene prevalence among 36 and 86 E. coli isolates from dogs without diarrhoea reported in Canada (Nakazato et al 2004) and Brazil (Puno-Sarmiento et al 2013), respectively. This finding further suggests higher rate of environmental contamination and dog infection with pathogenic E. coli strains in Iran than the other study areas.…”

Section: 4% a A D ( A 1 ) T E T ( A ) T E T ( B ) D F R ( A 1 ) S contrasting

confidence: 43%

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Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran

Torkan¹,

Bahadoranian²,

Khamesipour³

et al. 2016

Arch. med. vet.

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Section: 4% a A D ( A 1 ) T E T ( A ) T E T ( B ) D F R ( A 1 ) S contrasting

confidence: 43%

Section: 4% a A D ( A 1 ) T E T ( A ) T E T ( B ) D F R ( A 1 ) S contrasting

confidence: 43%

Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran

Torkan¹,

Bahadoranian²,

Khamesipour³

et al. 2016

Arch. med. vet.

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“…These two strains are believed to cause diarrhea due to A/E lesions, by means of close adherence to the intestinal epithelium, since strain SPA14 specifically had accumulation of actin in the bacterial adhesion sites, being positive by the fluorescent-actin staining test (FAS) 8 .…”

Section: Discussionmentioning

confidence: 99%

“…The available reports on the presence of virulence factors in samples of the typical EPEC group (eae, bfpA genes and EAF plasmid) from canine colibacillus isolates stimulate epidemiological studies since the dog is a domestic animal which is thus capable of transmitting this pathogen to humans 8 .…”

Section: Discussionmentioning

confidence: 99%

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Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pups

Gouveia¹,

Silva²,

Nakazato

et al. 2011

Acta Cir. Bras.

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Purpose:To verify the possibility of an experimental infection with enteropathogenic Escherichia coli and to confirm by PCR that the symptoms manifested after infection were due to the virulence factors of the studied bacteria. Methods: Experimental units were 14 healthy pups of Boxer breed, aged 60 days. The animals were divided into three groups. One animal from each litter was included in a control group and the remaining animals were divided into two groups: one inoculated with strain 4083, and another one inoculated with strain SPA14. Gelatinous capsules coated with enteric-coating solution were used for the inoculation of strains. E. coli isolation from feces was performed for all tested animals, and the extracted DNA was subjected to Polymerase Chain Reaction (PCR). Results: All infected animals presented diarrhea and had the gene eae amplified by PCR. Conclusion: The efficiency of PCR for the studied strains indicates that this technique can be recommended for the diagnosis of enteropathogenic Escherichia coli as a differential from other pathogens causing diarrhea. It may also be used in the future to verify whether other virulence factors (bfpA gene and EAF plasmid) persist after infection and to assess the pathogenicity of these bacteria. Key words: Enteropathogenic Escherichia coli. Virulence Factors. Polymerase Chain Reaction. Dogs. RESUMOObjetivo: Verificar a possibilidade de uma infecção experimental com Escherichia coli enteropatogênicas e confirmar por PCR que os sintomas manifestados após a infecção foram decorrentes dos fatores de virulência da bactéria estudada. Métodos: As unidades experimentais foram 14 filhotes saudáveis com idade de 60 dias da raça Boxer. Os animais foram divididos em três grupos, sendo um controle de cada ninhada e o restante dividido em dois grupos, um de animais inoculados com a cepa 4083 e o outro de animais inoculados com a cepa SPA14. Para inoculação das cepas, utilizaram-se cápsulas gelatinosas revestidas com solução de revestimento entérico. O isolamento de E. coli das fezes foi realizado em todos os animais testados, e o DNA extraído foi submetido à técnica de PCR. Resultados: Todos os animais infectados apresentaram diarréia e tiveram a gene eae amplificado por meio de PCR. Conclusão: Através da eficiência da PCR das amostras, a técnica seria recomendada para diagnóstico da Escherichia coli enteropatogênicas como diferencial de outros patógenos que causam diarréia, e, no futuro, verificar se outros fatores de virulência (gene bfpA e plasmídeo EAF) permaneceriam após a infecção, podendo avaliar a patogenicidade das EPEC. Descritores: Escherichia coli Enteropatogênica. Fatores de Virulência. Reação em Cadeia da Polimerase. Cães.

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Atypical Enteropathogenic Escherichia coli

Gomes

Yamamoto

Vieira

et al. 2016

Escherichia Coli in the Americas

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Attaching and effacing Escherichia coli isolated from dogs in Brazil: characteristics and serotypic relationship to human enteropathogenic E. coli (EPEC)

Cited by 53 publications

References 32 publications

Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran

Detection of virulence and antimicrobial resistance genes in Escherichia coli isolates from diarrhoiec dogs in Iran

Experimental infection with enteropathogenic Escherichia coli identified by PCR using enteric-coated capsules in boxer pups

Atypical Enteropathogenic Escherichia coli

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