AtSPO11-1 is necessary for efficient meiotic recombination in plants

Grelon, Mathilde

doi:10.1093/emboj/20.3.589

Cited by 450 publications

(574 citation statements)

References 71 publications

(124 reference statements)

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“…In contrast to the case of atxrcc3/ spo11-1, no chromosome fragmentation is observed in anaphase II of the atrad51/atspo11-1 double mutant (Li et al, 2004). Grelon et al (2001) reported the presence of bridges in anaphase I of the spo11-1 mutant but no bridges in anaphase II. These observations suggest that AtXrcc3, and presumably also AtRad51C, functions in a post-AtRad51 role in the repair Spo11-induced DSBs or DSBs in homologous chromosomes and sister chromatids damaged spontaneously or by a minor meiotic endonuclease other than Spo11.…”

Section: Discussionmentioning

confidence: 84%

“…Recently, Forget et al (2004) showed the localization of Xrcc3 at sites of DNA breaks within 10 min after exposure to g-irradiation and that such localization is independent of Rad51. In plants, meiosis is initiated by the formation of DSB, which is introduced by Spo11, in a manner similar to that in yeast (Grelon et al, 2001;Li et al, 2004). Further studies should be performed to determine the localization of AtRad51C and AtXrcc3 following DSB formation by Spo11 in meiosis to understand the function of AtRad51C and AtXrcc3 in HR.…”

Section: Discussionmentioning

confidence: 99%

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Arabidopsis RAD51C Gene Is Important for Homologous Recombination in Meiosis and Mitosis

et al. 2005

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Rad51 is a homolog of the bacterial RecA recombinase, and a key factor in homologous recombination in eukaryotes. Rad51 paralogs have been identified from yeast to vertebrates. Rad51 paralogs are thought to play an important role in the assembly or stabilization of Rad51 that promotes homologous pairing and strand exchange reactions. We previously characterized two RAD51 paralogous genes in Arabidopsis (Arabidopsis thaliana) named AtRAD51C and AtXRCC3, which are homologs of human RAD51C and XRCC3, respectively, and described the interaction of their products in a yeast two-hybrid system. Recent studies showed the involvement of AtXrcc3 in DNA repair and functional role in meiosis. To determine the role of RAD51C in meiotic and mitotic recombination in higher plants, we characterized a T-DNA insertion mutant of AtRAD51C. Although the atrad51C mutant grew normally during vegetative developmental stage, the mutant produced aborted siliques, and their anthers did not contain mature pollen grains. Crossing of the mutant with wild-type plants showed defective male and female gametogeneses as evidenced by lack of seed production. Furthermore, meiosis was severely disturbed in the mutant. The atrad51C mutant also showed increased sensitivity to g-irradiation and cisplatin, which are known to induce double-strand DNA breaks. The efficiency of homologous recombination in somatic cells in the mutant was markedly reduced relative to that in wild-type plants.

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Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Arabidopsis RAD51C Gene Is Important for Homologous Recombination in Meiosis and Mitosis

et al. 2005

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“…Similarly, a Mre11 mutant (the mre11-1 allele, truncated after amino acid 261, in the phosphoesterase motif IV) is viable, but sterile (Bundock & Hooykaas 2002). These mutants show no normal pachytene stage and a defect in homologous chromosome pairing and synapsis, and extensive meiotic chromosome fragmentation (Bleuyard et al 2004, Puizina et al 2004, which is not seen in a spo11 mutant (Grelon et al 2001). In addition, the fact that this fragmentation in mre11 mutant is Spo11-dependent clearly suggests that DSB are formed by Spo11 in the Arabidopsis Mre11 complex mutants, but that they are not repaired (Puizina et al 2004).…”

Section: Conservation Of the Roles Of The Mre11 Complex For Meiotic Rmentioning

confidence: 99%

The multiple roles of the Mre11 complex for meiotic recombination

2007

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During the first meiotic prophase, numerous DNA double-strand breaks (DSB) are formed in the genome in order to initiate recombination between homologous chromosomes. The conserved Mre11 complex, formed of Mre11, Rad50 and Nbs1 (Xrs2 in Saccharomyces cerevisiae) proteins, plays a crucial role in mitotic cells for sensing and repairing DSB. In meiosis the Mre11 complex is also required for meiotic recombination. Depending on the organisms, the Mre11 complex is required for the formation of the DSB catalysed by the transesterase Spo11 protein. It then plays a unique function in removing covalently attached Spo11 from the 5 ¶ extremity of the breaks through its nuclease activity, to allow further break resection. Finally, the Mre11 complex also plays a role during meiosis in bridging DNA molecules together and in sensing Spo11 DSB and activating the DNA damage checkpoint. In this article the different biochemical functions of the Mre11 complex required during meiosis are reviewed, as well as the consequences of Mre11 complex inactivation for meiosis in several organisms. Finally, I describe the meiotic phenotypes of several animal models that have been developed to model hypomorphic mutations of the Mre11 complex, involved in humans in some genetic instability disorders.

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“…Mutation in the Arabidopsis AtSPO11-1 gene shows abnormalities similar to those of the yeast spo11 mutants, including greatly reduced recombination and synapsis. However, the presence of residual bivalents indicates that there might be SPO11-independent pairing, synapsis, and/or recombination mechanisms [45].…”

Section: Resolution Of Dhjmentioning

confidence: 99%

Double-stranded DNA breaks and gene functions in recombination and meiosis

Mā

2006

Cell Res

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Gene functions in recombination and meiosis 402 npg REVIEW Meiotic prophase I is a long and complex phase. Homologous recombination is an important process that occurs between homologous chromosomes during meiotic prophase I. Formation of chiasmata, which hold homologous chromosomes together until the metaphase I to anaphase I transition, is critical for proper chromosome segregation. Recent studies have suggested that the SPO11 proteins have conserved functions in a number of organisms in generating sites of double-stranded DNA breaks (DSBs) that are thought to be the starting points of homologous recombination. Processing of these sites of DSBs requires the function of RecA homologs, such as RAD51, DMC1, and others, as suggested by mutant studies; thus the failure to repair these meiotic DSBs results in abnormal chromosomal alternations, leading to disrupted meiosis. Recent discoveries on the functions of these RecA homologs have improved the understanding of the mechanisms underlying meiotic homologous recombination.Cell Research (2006) Meiosis is a highly coordinated cell division, which generates four haploid reproductive cells required for sexual reproduction. Meiosis involves one round of DNA replication and two nuclear divisions, meiosis I and meiosis II. The first nuclear division leads to the segregation of homologous chromosomes (homologs), and is unique to meiosis. During the second division, sister chromatids are separated, resulting in the formation of four haploid nuclei and followed by the meiotic cytokinesis that forms four haploid cells. Similar to mitosis, both meiosis I and meiosis II can be divided into four stages: prophase, metaphase, anaphase, and telophase.However, meiosis I differs from meiosis II as meiosis I involves homology search, pairing, interhomolog recombination, and synapsis of homologs, processes that are required for correct association and segregation of homologs. The prophase stage of meiosis I, or prophase I, has been the target of interest because of the occurrence of these major processes of chromosome interactions. During early prophase I, arms of sister chromosomes are closely associated by protein complexes called cohesins, which are removed during the metaphase I/anaphase I transition. However, the centromere regions remained associated until the segregation of sister chromatids during meiosis II. Homologous pairing is the result of interaction between homologous chromosomes relying on the homology of DNA sequences [1,2], and is considered to be a transient and non-stable association between homologous chromosomes. Pairing between homologous chromosomes facilitates the process of recombination. The recombination process confers the interexchange of genetic information between non-sister chromatids, and generates chiasmata, which ensure proper association between homologous chromosomes prior to chromosome segregation at anaphase I. Synapsis, however, is a stable association by forming synaptonemal complexes between chromosomes. Synaptonemal complexes are threeparti...

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AtSPO11-1 is necessary for efficient meiotic recombination in plants

Cited by 450 publications

References 71 publications

Arabidopsis RAD51C Gene Is Important for Homologous Recombination in Meiosis and Mitosis

Arabidopsis RAD51C Gene Is Important for Homologous Recombination in Meiosis and Mitosis

The multiple roles of the Mre11 complex for meiotic recombination

Double-stranded DNA breaks and gene functions in recombination and meiosis

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