AtS40-1, a group I DUF584 protein positively regulates ABA response and salt tolerance in Arabidopsis

Wang, Yating; Li, Yingying; Tian, Hainan; Wang, Wei; Wang, Xutong; Hussain, Saddam; Yuan, Yuan; Lin, Rongcheng; Hussain, Hadia; Wang, Tianya; Wang, Shucai

doi:10.1016/j.gene.2022.146846

Cited by 5 publications

(3 citation statements)

References 48 publications

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“…For example, WRKY transcription factor 22 (WRKY22), which plays a crucial role in the dark‐induced senescence (Zhou et al., 2011), is downregulated in mpk1/2/7/14 and mbd10‐2 . Protein S40‐3 (AtS40‐3) is also downregulated and it is known that AtS40‐3 is induced in response to aging, dark treatment, ABA, salicylic acid, and pathogen infection (Anwer et al., 2018; Fischer‐Kilbienski et al., 2010; Jehanzeb et al., 2017; Wang et al., 2022). Another example is 12‐oxophytodienoate reductase 1 (OPR1), which is involved in leaf senescence and JA response (Biesgen & Weiler, 1999; He & Gan, 2001).…”

Section: Resultsmentioning

confidence: 99%

Group C MAP kinases phosphorylate MBD10 to regulate ABA‐induced leaf senescence in Arabidopsis

Li,

Kamiyama,

Minegishi

et al. 2024

The Plant Journal

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SUMMARYAbscisic acid (ABA) is a phytohormone that promotes leaf senescence in response to environmental stress. We previously identified methyl CpG‐binding domain 10 (MBD10) as a phosphoprotein that becomes differentially phosphorylated after ABA treatment in Arabidopsis. ABA‐induced leaf senescence was delayed in mbd10 knockout plants but accelerated in MBD10‐overexpressing plants, suggesting that MBD10 positively regulates ABA‐induced leaf senescence. ABA‐induced phosphorylation of MBD10 occurs in planta on Thr‐89, and our results demonstrated that Thr‐89 phosphorylation is essential for MBD10's function in leaf senescence. The in vivo phosphorylation of Thr‐89 in MBD10 was significantly downregulated in a quadruple mutant of group C MAPKs (mpk1/2/7/14), and group C MAPKs directly phosphorylated MBD10 in vitro. Furthermore, mpk1/2/7/14 showed a similar phenotype as seen in mbd10 for ABA‐induced leaf senescence, suggesting that group C MAPKs are the cognate kinases of MBD10 for Thr‐89. Because group C MAPKs have been reported to function downstream of SnRK2s, our results indicate that group C MAPKs and MBD10 constitute a regulatory pathway for ABA‐induced leaf senescence.

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Section: Resultsmentioning

confidence: 99%

Group C MAP kinases phosphorylate MBD10 to regulate ABA‐induced leaf senescence in Arabidopsis

Li,

Kamiyama,

Minegishi

et al. 2024

The Plant Journal

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“…This study's transcriptome analysis also demonstrated that genes expressed in leaves at the full heading stage are signi cantly enriched in senescence, primarily involving proteins containing the DUF584 domain, associated with aging-associated cysteine proteases and precursors and related cofactors. Proteins containing the DUF584 domain played an essential role in rice aging, regulating the synthesis and breakdown of abscisic acid within the plant [53]. Cysteine aids in promoting GSH formation and scavenging superoxide radicals, revealing the molecular mechanisms by which long-term fertilization with chemical and organic fertilizers regulates the content of MDA, Pro, and other substances, delaying rice aging, enhancing dry matter accumulation, and improving yield formation.…”

Section: Impact Of Long-term Fertilization On the Yield Of Early Ricementioning

confidence: 99%

Effect of long-term fertilization on the growth and yield formation of early rice

Hu,

Liu,

et al. 2024

Preprint

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Fertilization is crucial for rice growth and yield formation. We conducted a 42-year long-term fixed experiment in southeast China, examining nine treatments. This study focused on three treatments: a combination of chemical N, P, and K (NPK), a double dose of chemical N, P, and K (HNPK), and a combination of chemical fertilizer and organic fertilizers (NPKM). We assessed rice yield, yield components, tiller dynamics, dry matter accumulation, chlorophyll dynamics, and leaf transcriptome at the full heading stage. Results indicated that early rice yield followed the order of NPKM > HNPK > NPK. Compared to NPK, HNPK and NPKM significantly increased spikelet density, effective panicles, and 1000-grain weight, while also promoting tillering. NPKM and HNPK significantly enhanced dry matter accumulation from the full heading stage to the filling stage and facilitated the transport of dry matter from leaves and stems to spikes during the filling to mature stages. NPKM consistently maintained higher chlorophyll content than HNPK and NPK at all stages, significantly reducing chlorophyll decline from the full heading stage to the filling stage. Correlation analysis revealed a significant positive relationship between yield and both chlorophyll content and dry matter accumulation under long-term fertilization. There was also a significant negative correlation between yield and chlorophyll reduction from the full heading stage to the filling stage. Differential gene expression analysis at the full heading stage showed significant enrichment in photosynthesis and plant senescence metabolism pathways among different fertilization treatments. Overall, the combined application of chemical and organic fertilizers significantly increased early rice yield by enhancing tillering, regulating photosynthesis and senescence-related gene expression, boosting dry matter accumulation from the full heading stage to the filling stage, and improving dry matter transport to spikes from the filling to the mature stage.

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“…ABA inhibited seed germination and seedling greening were assayed as described previously [33,34,37,51]. Briefly, surface sterilized seeds of the Col wild type, the 35S:BIC1 and 35S:BIC2 transgenic plants, the bic1 and bic2 single, and the bic1 bic2 double mutants were sown on plates with 1 /2 MS salts solidified with 1.0% agar in the presence or absence of 1 µM ABA, kept at 4 • C in darkness for 2 days, and then transferred to a growth room.…”

Section: Aba Sensitivity Assaysmentioning

confidence: 99%

BIC2, a Cryptochrome Function Inhibitor, Is Involved in the Regulation of ABA Responses in Arabidopsis

Wang

Jia

et al. 2023

Plants

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The plant hormone ABA (abscisic acid) is able to regulate plant responses to abiotic stresses via regulating the expression of ABA response genes. BIC1 (Blue-light Inhibitor of Cryptochromes 1) and BIC2 have been identified as the inhibitors of plant cryptochrome functions, and are involved in the regulation of plant development and metabolism in Arabidopsis . In this study, we report the identification of BIC2 as a regulator of ABA responses in Arabidopsis . RT-PCR (Reverse Transcription-Polymerase Chain Reaction) results show that the expression level of BIC1 remained largely unchanged, but that of BIC2 increased significantly in response to ABA treatment. Transfection assays in Arabidopsis protoplasts show that both BIC1 and BIC2 were mainly localized in the nucleus, and were able to activate the expression of the co-transfected reporter gene. Results in seed germination and seedling greening assays show that ABA sensitivity was increased in the transgenic plants overexpressing BIC2, but increased slightly, if any, in the transgenic plants overexpressing BIC1. ABA sensitivity was also increased in the bic2 single mutants in seedling greening assays, but no further increase was observed in the bic1 bic2 double mutants. On the other hand, in root elongation assays, ABA sensitivity was decreased in the transgenic plants overexpressing BIC2, as well as the bic2 single mutants, but no further decrease was observed in the bic1 bic2 double mutants. By using qRT-PCR (quantitative RT-PCR), we further examined how BIC2 may regulate ABA responses in Arabidopsis , and found that inhibition of ABA on the expression of the ABA receptor genes PYL4 (PYR1-Like 4) and PYL5 were decreased, but promotion of ABA on the expression of the protein kinase gene SnRK2.6 (SNF1-Related Protein Kinases 2.6) was enhanced in both the bic1 bic2 double mutants and 35S:BIC2 overexpression transgenic plants. Taken together, our results suggest that BIC2 regulates ABA responses in Arabidopsis possibly by affecting the expression of ABA signaling key regulator genes.

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AtS40-1, a group I DUF584 protein positively regulates ABA response and salt tolerance in Arabidopsis

Cited by 5 publications

References 48 publications

Group C MAP kinases phosphorylate MBD10 to regulate ABA‐induced leaf senescence in Arabidopsis

Group C MAP kinases phosphorylate MBD10 to regulate ABA‐induced leaf senescence in Arabidopsis

Effect of long-term fertilization on the growth and yield formation of early rice

BIC2, a Cryptochrome Function Inhibitor, Is Involved in the Regulation of ABA Responses in Arabidopsis

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