Atropa belladonna Hairy Roots: Orchestration of Concurrent Oxidation and Reduction Reactions for Biotransformation of Carbonyl Compounds

Srivastava, Vikas; Negi, Arvind S.; Ajayakumar, P. V.; Khan, Shahanavaj; Banerjee, Sayan

doi:10.1007/s12010-011-9533-3

Cited by 15 publications

(2 citation statements)

References 24 publications

(40 reference statements)

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“…More recently, it was demonstrated for the first time the untapped potentials of a selected HR clone from Atropa belladonna to perform simultaneous oxidation and reduction of 3,4,5-trimethoxy benzaldehyde into 3,4,5-trimethoxy benzoic acid and 3,4,5-trimethoxy benzyl alcohol, respectively. The same HR clone also demonstrated the ability to reduce 3,4,5-trimethoxyacetophenone into the secondary alcohol, while not biotransform the carboxylic acid substrate under similar bio-reaction conditions [98]. All these examples demonstrate the great potential of HRs as model system for in vitro biotransformations of exogenous substrates.…”

Section: Hrs As An Important Tool For Biotransfomation Of Exogenous S...mentioning

confidence: 74%

Hairy Roots, their Multiple Applications and Recent Patents

Talano

Oller²,

González³

et al. 2012

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In the last years, hairy root (HR) cultures are gaining attention in the biotechnology industry. This particular plant cell culture derives from explants infected with Agrobacterium rhizogenes. They constitute a relatively new approach to in vitro plant biotechnology and modern HR cultures are far away from the valuables findings performed by Philip R. White in the 1930's, who obtained indefinite growth of excised root tips. HR cultures are characterized by genetic and biochemical stability and high growth rate without expensive exogenous hormones source. HR cultures have allowed a deep study of plant metabolic pathways and the production of valuable secondary metabolites and enzymes, with therapeutic or industrial application. Furthermore, the potential of HR cultures is increasing continuously since different biotechnological strategies such as genetic engineering, elicitation and metabolic traps are currently being explored for discovery of new metabolites and pathways, as well as for increasing metabolites biosynthesis and/or secretion. Advances in design of proper bioreactors for HR growth are being of great interest, since scale up of metabolite production will allow the integration of this technology to industrial processes. Another application of HR cultures is related to their capabilities to biotransform and to degrade different xenobiotics. In this context, removal assays using this plant model system are useful tools for phytoremediation assays, previous to the application in the field. This review highlights the more recent application of HRs and those new patents which show their multiple utilities.

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Section: Hrs As An Important Tool For Biotransfomation Of Exogenous S...mentioning

confidence: 74%

Hairy Roots, their Multiple Applications and Recent Patents

Talano

Oller²,

González³

et al. 2012

BIOT

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“…Recently, seven distinct kinds of biotransformation reactions like glycosylation, glucosylation, reduction, condensation, etc., were classified as major reaction types that have been reported . Besides, HRCs of some plants like Pharbitis nil, Brassica napus, and Atropa belladonna bear the potential of more than one type of chemical conversion simultaneously Srivastava et al 2012Srivastava et al , 2013. These abilities actually strengthen the use of HRCs as a model system for in vitro biotransformation of exogenous substrates.…”

Section: Hairy Root-based Value-added Applicationsmentioning

confidence: 98%

Hairy root biotechnology—indicative timeline to understand missing links and future outlook

et al. 2015

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Agrobacterium rhizogenes-mediated hairy roots (HR) were developed in the laboratory to mimic the natural phenomenon of bacterial gene transfer and occurrence of disease syndrome. The timeline analysis revealed that during 90 s, the research expanded to the hairy root-based secondary metabolite production and different yield enhancement strategies like media optimization, up-scaling, metabolic engineering etc. An outlook indicates that much emphasis has been given to the strategies that are helpful in making this technology more practical in terms of high productivity at low cost. However, a sequential analysis of literature shows that this technique is upgraded to a biotechnology platform where different intra- and interdisciplinary work areas were established, progressed, and diverged to provide scientific benefits of various hairy root-based applications like phytoremediation, molecular farming, biotransformation, etc. In the present scenario, this biotechnology research platform includes (a) elemental research like hairy root-mediated secondary metabolite production coupled with productivity enhancement strategies and (b) HR-based functional research. The latter comprised of hairy root-based applied aspects such as generation of agro-economical traits in plants, production of high value as well as less hazardous molecules through biotransformation/farming and remediation, respectively. This review presents an indicative timeline portrayal of hairy root research reflected by a chronology of research outputs. The timeline also reveals a progressive trend in the state-of-art global advances in hairy root biotechnology. Furthermore, the review also discusses ideas to explore missing links and to deal with the challenges in future progression and prospects of research in all related fields of this important area of plant biotechnology.

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