Atriplex mollis Desf. Aerial Parts: Extraction Procedures, Secondary Metabolites and Color Analysis

Abstract

A method using high-performance liquid chromatography coupled with a photodiode array detector was proposed for the rapid characterization of different phenolic constituents from the extracts of Atriplex mollis aerial parts. Atriplex species are known for their multiple biological activities, but no information is available in the literature about A. mollis. With the aim to firstly characterize the main secondary metabolites of this plant, so as to orient better the biological evaluation, we applied three different extraction procedures and compared the chromatographic results. Microwave-assisted extraction gave the best yield and recovery of important compounds such as gallic acid, catechin, chlorogenic acid, p-OH benzoic acid, rutin, sinapinic acid, t-ferulic acid, naringenin and benzoic acid. These constituents belong to three important chemical classes: phenolic acids, flavonoids and monoterpenes. Color evaluation and analysis of chlorophylls (a and b) and carotenoids complete the preliminary profile of this plant. From these analyses, Atriplex mollis is a source of bioactive compounds (especially rutin, t-ferulic acid and gallic acid) and could be recommended as a plant of phyto-pharmaceutical relevance, opening new perspectives on this salt-tolerant plant.

Chromatographic Analyses, In Vitro Biological Activities, and Cytotoxicity of Cannabis sativa L. Essential Oil: A Multidisciplinary Study

“…Color interpretation was based on the lightness L* (between 0, absolute black, and 100, absolute white), the greenness (negative) or redness (positive) a*, and the blueness (negative) or yellowness (positive) b*. The chroma, or saturation ( C * ab ) quantitatively describes the color intensity, and the hue or color angle ( h ab ) is the attribute of appearance by which a color is identified according to its resemblance to red, yellow, green, blue, or a combination of two of these [12]. The results are reported as the mean value ± standard deviation (SD).…”

Liquid Phase and Microwave-Assisted Extractions for Multicomponent Phenolic Pattern Determination of Five Romanian Galium Species Coupled with Bioassays

“…In the present study, following our research on innovative microextraction procedures [21,22,23,24,25,26,27], different microextraction procedures were examined for the analysis of phenolic compounds in G. verum aerial parts, and then applied for the determination of the phenolic pattern of four other Galium species ( G. album , G. rivale , G. pseudoaristatum , and G. purpureum ). As an alternative, the microwave-assisted extraction (MAE) technique was used as a reference method [27,28,29].…”

Tagetes spp. Essential Oils and Other Extracts: Chemical Characterization and Biological Activity

“…Natural products proved their importance as sources of substances with therapeutic potential [ 2 , 6 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 ]. Plant medicines have some advantages, because their components act on different molecular targets, incorporating lower cost and fewer side effects [ 2 , 16 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ].…”