Atrioventricular groove separation is a rare complication of mitral valve surgery. We present a case occurring during aortic valve surgery that was detected by the anesthesiologist using transesophageal echocardiography (TEE). During weaning from cardiopulmonary bypass after uneventful replacement of a severely calcified aortic valve, blood flow was seen on TEE leaving the left ventricular outflow tract just below the annulus of the aortic valve. This blood flow expanded into the aorto-mitral curtain during systole, consistent with atrioventricular groove separation. We review the pathophysiology behind groove separations and discuss diagnosis of these rare conditions using TEE.