Atrial Natriuretic Peptide as an Indicator of the Severity of Valvular Regurgitation and Heart Failure in Horses

Leroux, Aurélia; Haidar, Ali Al; Rémy, B.; Borde, Laura; Cerri, Simona; Houben, Rosa; Sandersen, Charlotte; Moula, Nassim; Erck-Westergren, E. van; Amory, Hélène

doi:10.1016/j.jevs.2014.08.007

Cited by 7 publications

(6 citation statements)

References 37 publications

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“…The present study also proposed no significant relationship between the weight and age of the horses with plasma concentrations of ANP, BNP and endothelin‐1 (Table 4). This finding was in harmony with the result of the study performed by Leroux et al (2014).…”

Section: Discussionsupporting

confidence: 92%

“…They reported a nonsignificant increase in ANP with atrial and ventricular dilatation in horses with heart valve regurgitations. But, Leroux et al (2014) suggested measurement of ANP has a diagnostic value in horses with valvular regurgitation with the atrial dilation because ANP level was significantly related to the diameter of the left atrial.…”

Section: Discussionmentioning

confidence: 99%

“…Because of this, most of the research which studied plasma concentration of ANP and BNP in horses was performed using human kits (Nakagawa et al, 2019; Van Der Vekens et al, 2013). Evaluation of ANP concentration in horses using nonspecific kits demonstrated higher plasma concentration of ANP in horses with heart diseases and valvular regurgitation, especially mitral valve regurgitation, accompanied by the altered size of the left atrium and ventricle (Leroux et al, 2014; Trachsel et al, 2012, 2013, 2015; Van Der Vekens et al, 2016). However measurement of BNP in horses using kits available for another species does not show any significant differences between healthy horses and horses with cardiovascular diseases (Van Der Vekens, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Measurement of ANP, BNP and endothelin‐1 concentrations in jumping horses with heart valvular regurgitation and their correlation with the dimensions of heart structures

Chaleshtori

Dezfouli

Najizadeh

et al. 2022

Equine Veterinary Education

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Summary Background Natriuretic peptides and endothelin‐1 are used as biochemical biomarkers for the diagnosis and prognosis of heart diseases. Objectives This study aimed to investigate plasma ANP, BNP and endothelin‐1 in jumping horses with various manifestations of heart valve regurgitation and their association with echocardiographic variables. Study design Clinical evaluations including cardiac auscultation were performed in 198 jumping horses, and 30 horses were chosen. Methods Sixteen jumping horses with murmurs of grade 3/6 to 5/6 having various degrees of heart valve regurgitation with the severity of insignificant to moderate on colour Doppler echocardiography were considered as the valvular regurgitation group (average age 9.75 ± 3.28 years and weight 427.43 ± 82.22 kg, and consisting of 10 mares, three stallions and three castrated male horses with breeds of 12 Thoroughbred and four crossbred). Fourteen healthy horses were chosen as the healthy group (average age 8.64 ± 2.44 years and weight 462.14 ± 77.42 kg, and consisting of nine mares, three stallions and two castrated males with breeds of 12 Thoroughbreds, and two crossbred). Blood samples were collected from the jugular vein, and plasma concentration of ANP, BNP and endothelin‐1 was determined using Sandwich ELISA by a horse‐specific kit. Then, the plasma concentration of mentioned peptides and echocardiographic variables were compared between the two groups. Results B‐type natriuretic peptide was significantly increased in horses with valve regurgitation compared to healthy horses, but increases in ANP and endothelin‐1 were insignificant. Also, in the valvular regurgitation group, horses with a pulmonic valve disorder had a significantly higher concentration of BNP and endothelin‐1 than healthy horses. Assessment of the relation between ANP, BNP and endothelin‐1 with echocardiographic variables showed a significant correlation between ANP and right ventricle length (RVL) and left ventricle width (LVW) in systole and endothelin‐1 with right ventricle width (RVW) and left ventricle width in systole, but BNP had no significant correlation. Conclusion and clinical importance According to the study results, it can be assumed that the measurement of these cardiac biomarkers can be helpful in the diagnosis of the jumping horse with cardiac valvular disorders (especially pulmonic valve regurgitation) and changes in the dimensions of the heart ventricle.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Measurement of ANP, BNP and endothelin‐1 concentrations in jumping horses with heart valvular regurgitation and their correlation with the dimensions of heart structures

Chaleshtori

Dezfouli

Najizadeh

et al. 2022

Equine Veterinary Education

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“…A higher proANP concentration has been found in dogs and human beings with congestive heart failure (Hunter and others 2011, Lauridsen and others 2013), but the applicability in horses remains untested. Since ANP release has already been described in horses with atrial dilatation (Trachsel and others 2012, Leroux and others 2014), the first aim of this study was to determine reference values for proANP in horses and to establish a cut‐off value for detection of atrial dilatation. The second aim of the present study was to examine the stability of proANP samples by comparison of storage at −80°C and −20°C as a previous equine ANP study (Van Der Vekens and others 2014) has demonstrated that ANP samples are only stable at −80°C.…”

Section: Introductionmentioning

confidence: 99%

Total plasma proANP increases with atrial dilatation in horses

et al. 2015

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Equine atrial natriuretic peptide (ANP) plasma concentrations are correlated with left atrial size. However, species-specific assays are lacking and the results from human assays are poorly reproducible. A new methodology called processing independent analysis (PIA) that measures the total proANP product in plasma has proven to be successful in human medicine, but has never been used in horses. The aims were to establish an equine proANP reference interval by measurement of the total proANP product using PIA and to examine the proANP concentrations in horses with atrial dilatation. Sample stability was studied by comparison of storage at -80°C and -20°C. Plasma samples were obtained from 23 healthy horses, 12 horses with moderate or severe valvular regurgitation without atrial dilatation and 42 horses with valvular regurgitation and atrial dilatation. The proANP concentration was significantly (P<0.001) higher in horses with atrial dilatation (761.4 (442.1-1859.1) pmol/l) than in healthy horses (491.6 (429.5-765.9) pmol/l; P<0.001) or horses with cardiac disease but without atrial dilatation (544.4 (457.0-677.6) pmol/l). A cut-off value (573.8 pmol/l) for detection of atrial dilatation was calculated. Sample storage at -80°C did not differ from sample storage at -20°C. The measurement of total proANP in plasma detects atrial dilatation in horses and may be useful for clinical evaluation in equine medicine.

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“…Van der Vekens and others provide a short and concise review of ANP assays already used in horses. Despite promising preliminary results showing a correlation between ANP concentration and left atrial dilatation (Leroux and others 2014), major limitations such as ANP instability and poor reproducible results with human ANP assays (Trachsel and others 2014) still prevent its clinical use in horses.…”

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confidence: 99%