Atrial natriuretic factor is a circulating hormone

Gutkowska, Jolanta; Horký, K; Thibault, Gaétan; Januszewicz, Paweł; Cantin, M.; Genest, Jacques

doi:10.1016/s0006-291x(84)80370-8

Cited by 136 publications

(50 citation statements)

References 15 publications

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“…Plasma levels of ANP, OT, and VP were measured by radioimmunoassay as previously described (16,23,24). VP and OT were extracted from 1 ml of plasma with acetone and petroleum ether, and ANP was extracted from 1 ml of plasma using Sep-Pak C-18 cartridges (Waters, Milford, MA).…”

Section: Determination Of Plasma Anp Ot and Vp Concentrationsmentioning

confidence: 99%

Serotonergic mechanisms of the lateral parabrachial nucleus in renal and hormonal responses to isotonic blood volume expansion

Margatho

Giusti‐Paiva²,

Menani³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

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This study investigated the involvement of serotonergic mechanisms of the lateral parabrachial nucleus (LPBN) in the control of sodium (Na ϩ ) excretion, potassium (K ϩ ) excretion, and urinary volume in unanesthetized rats subjected to acute isotonic blood volume expansion (0.15 M NaCl, 2 ml/100 g of body wt over 1 min) or control rats. Plasma oxytocin (OT), vasopressin (VP), and atrial natriuretic peptide (ANP) levels were also determined in the same protocol. Male Wistar rats with stainless steel cannulas implanted bilaterally into the LPBN were used. In rats treated with vehicle in the LPBN, blood volume expansion increased urinary volume, Na ϩ and K ϩ excretion, and also plasma ANP and OT. Bilateral injections of serotonergic receptor antagonist methysergide (1 or 4 g/200 l) into the LPBN reduced the effects of blood volume expansion on increased Na ϩ and K ϩ excretion and urinary volume, while LPBN injections of serotonergic 5-HT2a/HT2c receptor agonist, 2.5-dimetoxi-4-iodoamphetamine hydrobromide (DOI; 1 or 5 g/200 l) enhanced the effects of blood volume expansion on Na ϩ and K ϩ excretion and urinary volume. Methysergide (4 g) into the LPBN decreased the effects of blood volume expansion on plasma ANP and OT, while DOI (5 g) increased them. The present results suggest the involvement of LPBN serotonergic mechanisms in the regulation of urinary sodium, potassium and water excretion, and hormonal responses to acute isotonic blood volume expansion. serotonin; natriuresis; atrial natriuretic peptide; oxytocin; vasopressin ISOTONIC BLOOD VOLUME expansion, by reflex mechanisms, induces a series of regulatory responses, including the inhibition of the sympathetic outflow to the kidney, heart, and blood vessels, reduction of renin and vasopressin (VP) secretion, and increase in the levels of circulating atrial natriuretic peptide (ANP) and oxytocin (OT) that leads to diuresis and natriuresis (1,2,12,14,20,24,45,53).There are several reports demonstrating the correlation between natriuresis and diuresis and plasma ANP levels in response to systemic volume expansion, e.g., atrial balloon inflation, blood volume expansion, salt load, and water immersion (4,7,17,32,50,59). These responses depend on the activation of sinoaortic baroreceptors and/or cardiopulmonary receptor afferents located in the atria, lungs, great veins, and ventricles that project to the nucleus of the solitary tract (NTS) (1, 12, 22). The NTS neurons convey blood volume expansion signals and project to and excite different areas within the forebrain and hindbrain to produce neuroendocrine responses. Studies using expression of the immediate early gene c-Fos as a marker of neuronal activation have shown that, in addition to the NTS, volume-loading activates the paraventricular (PVN) and supraoptic (SON) nuclei, dorsal raphe nucleus (DRN), caudal ventrolateral medulla, locus coeruleus, and also the lateral parabrachial nucleus (LPBN) (6,20,46). The LPBN, a structure lying dorsolateral to the superior cerebellar peduncle, has been shown to play an ...

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Section: Determination Of Plasma Anp Ot and Vp Concentrationsmentioning

confidence: 99%

Serotonergic mechanisms of the lateral parabrachial nucleus in renal and hormonal responses to isotonic blood volume expansion

Margatho

Giusti‐Paiva²,

Menani³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

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“…It is found within ultrastructural granules in the atria that anatomically resemble secretory granules of endocrine tissues (5), and is derived from a larger precursor molecule as are many peptide hormones (6). Three recent reports have described radioimmunoassays (RIAs) for ANF in rat plasma (7)(8)(9), and levels of ANF were higher in rats on high sodium diet than on low sodium diet (8).…”

Section: Introductionmentioning

confidence: 99%

Plasma levels of immunoreactive atrial natriuretic factor in healthy subjects and in patients with edema.

Shenker¹,

Sider²,

Ostafin³

et al. 1985

J. Clin. Invest.

330

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MethodsAtrial natriuretic factor (ANF), a recently sequenced cardiac peptide, has been shown to have potent natriuretic, diuretic, and vasodilating effects in several species. We have developed a radioimmunoassay to measure the levels of immunoreactive ANF in human plasma. Plasma levels of ANF in healthy volunteers on a low sodium diet were 9.8±1.4 pmol/liter and increased to 21.9±3.0 on a high sodium diet. The levels of atrial natriuretic factor correlated directly with urinary sodium and inversely with plasma renin activity and plasma aldosterone levels. Patients with marked edema due to congestive heart failure had plasma levels of atrial natriuretic factor five times higher than normal (P < 0.05), whereas patients with cirrhosis and edema had levels that were not different from normal. These results suggest that atrial natriuretic factor plays an important role in the adaptation to increased sodium intake.

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“…Immunoreactive ANP was extracted from 1 ml of plasma with heatactivated Vycor glass beads (Corning No. 7930, Mesh 140) as described by Gutkowska et al (13), and the lyophilized residue was frozen at -20 o C. Each sample was resuspended in 500 µl ANP buffer (50 mM potassium phosphate, pH 7.4, 0.15 M NaCl, 0.1% bovine serum albumin (Sigma, A-7888), 0.1% Triton X-100, and 0.02% NaN 3 ) and aliquots of 100 µl were taken in duplicate for radioimmunoassay. After decapitation and blood collection as described above, AP, PP, ME, MBH, AV 3 V, OB, RA and LA were quickly removed and placed in 0.5 ml 0.1 M acetic acid containing the same proteolytic enzyme inhibitors used for plasma preparation.…”

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confidence: 99%

“…The remaining supernatant was lyophilized and stored at -20 o C until assay for ANP. Each sample was reconstituted in an appropriate volume of ANP buffer, and aliquots of 100 µl were taken for radioimmunoassay (13). The sensitivity and specificity of the ANP radioimmunoassay have been described in detail by Gutkowska et al (14).…”

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confidence: 99%

Correlations between ANP concentrations in atria, plasma and cerebral structures and sodium chloride preference in Wistar rats

Glass

Gutkowska²,

Favaretto³

et al. 1997

Braz J Med Biol Res

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We determined whether ANP (atrial natriuretic peptide) concentrations, measured by radioimmunoassay, in the ANPergic cerebral regions involved in regulation of sodium intake and excretion and pituitary gland correlated with differences in sodium preference among 40 Wistar male rats (180-220 g). Sodium preference was measured as mean spontaneous ingestion of 1.5% NaCl solution during a test period of 12 days. The relevant tissues included the olfactory bulb (OB), the posterior and anterior lobes of the pituitary gland (PP and AP, respectively), the median eminence (ME), the medial basal hypothalamus (MBH), and the region anteroventral to the third ventricle (AV 3 V). We also measured ANP content in the right (RA) and left atrium (LA) and plasma. The concentrations of ANP in the OB and the AP were correlated with sodium ingestion during the preceding 24 h, since an increase of ANP in these structures was associated with a reduced ingestion and vice-versa (OB: r = -0.3649, P<0.05; AP: r = -0.3291, P<0.05). Moreover, the AP exhibited a correlation between ANP concentration and mean NaCl intake (r = -0.4165, P<0.05), but this was not the case for the OB (r = 0.2422). This suggests that differences in sodium preference among individual male rats can be related to variations of AP ANP level. Earlier studies indicated that the OB is involved in the control of NaCl ingestion. Our data suggest that the OB ANP level may play a role mainly in day-today variations of sodium ingestion in the individual rat.

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Atrial natriuretic factor is a circulating hormone

Cited by 136 publications

References 15 publications

Serotonergic mechanisms of the lateral parabrachial nucleus in renal and hormonal responses to isotonic blood volume expansion

Serotonergic mechanisms of the lateral parabrachial nucleus in renal and hormonal responses to isotonic blood volume expansion

Plasma levels of immunoreactive atrial natriuretic factor in healthy subjects and in patients with edema.

Correlations between ANP concentrations in atria, plasma and cerebral structures and sodium chloride preference in Wistar rats

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