Atrial fibrillation and microRNAs

Santulli, Gaetano; Iaccarino, Guido; Luca, Nicola De; Trimarco, Bruno; Condorelli, Gianluigi

doi:10.3389/fphys.2014.00015

Cited by 121 publications

(117 citation statements)

References 128 publications

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“…Nonetheless, studies on the best normalization strategy in cardiac tissue remain sparse252627, and, to the best of our knowledge, no previous study has been performed to identify appropriate reference genes in the human atrial tissue for the study of AF. The lack of a general consensus on the best normalization strategy in this context has significant impact, considering the large increase in the number of studies analyzing the relationship between miRNA and AF in the last five years789. A variety of normalization strategies has been applied in different studies, which however were not validated in the specific experimental context of application2829.…”

Section: Discussionmentioning

confidence: 99%

“…AF is a multi-factorial disease, supported by altered electrophysiological and structural factors6. A growing body of works suggests miRNAs to act as potential mediators in the electrophysiological and structural remodeling of the atria maintaining AF789.…”

mentioning

confidence: 99%

“…Since no normalization standard has been proven to be ideal yet, it is essential to verify the expression stability of putative normalizers in each experimental setup, and in relation to the specific tissue, species, and disease under investigation. Despite the growing number of studies investigating miRNA expression in human AF789, at present no consensus exists on the reference genes for human atrial tissue samples. This may limit study comparison and, most importantly, lead to ambiguous data interpretation and misleading biological conclusions.…”

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confidence: 99%

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Selection of reference genes is critical for miRNA expression analysis in human cardiac tissue. A focus on atrial fibrillation

Masè

Grasso

Avogaro

et al. 2017

Sci Rep

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MicroRNAs (miRNAs) are emerging as key regulators of complex biological processes in several cardiovascular diseases, including atrial fibrillation (AF). Reverse transcription-quantitative polymerase chain reaction is a powerful technique to quantitatively assess miRNA expression profile, but reliable results depend on proper data normalization by suitable reference genes. Despite the increasing number of studies assessing miRNAs in cardiac disease, no consensus on the best reference genes has been reached. This work aims to assess reference genes stability in human cardiac tissue with a focus on AF investigation. We evaluated the stability of five reference genes (U6, SNORD48, SNORD44, miR-16, and 5S) in atrial tissue samples from eighteen cardiac-surgery patients in sinus rhythm and AF. Stability was quantified by combining BestKeeper, delta-Cq, GeNorm, and NormFinder statistical tools. All methods assessed SNORD48 as the best and U6 as the worst reference gene. Applications of different normalization strategies significantly impacted miRNA expression profiles in the study population. Our results point out the necessity of a consensus on data normalization in AF studies to avoid the emergence of divergent biological conclusions.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Selection of reference genes is critical for miRNA expression analysis in human cardiac tissue. A focus on atrial fibrillation

Masè

Grasso

Avogaro

et al. 2017

Sci Rep

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“…The prevalence of atrial fibrillation reaches values of 17.8% in people >85 years [1, 67, 68]. The development of a non-regular pattern of electrical activity might have detrimental consequences in hearts that are relatively stiff and relax slowly [1, 69, 70].…”

Section: Introductionmentioning

confidence: 99%

Adrenergic signaling in heart failure and cardiovascular aging

Santulli

Iaccarino

2016

Maturitas

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Both cardiovascular disease and aging are associated with changes in the sympathetic nervous system. Indeed, mounting evidence indicates that adrenergic receptors are functionally involved in numerous processes underlying both aging and cardiovascular disorders, in particular heart failure. This article will review the pathophysiological role of the sympathetic nervous system in heart failure and cardiovascular aging.

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“…In addition, recent studies showed the microRNAs might play important roles in the adaptive process of the failing heart and affect the treatment response of cardiac arrhythmia via electrical membrane signaling and ion channel activation. [42–44] A previous study also reported that metabolic syndrome was associated with poor outcome after PVC ablation. [45] This result emphasizes the role of metabolic, inflammatory, and redox processes, and ionic channel conduction properties on cardiac arrhythmia.…”

Section: Discussionmentioning

confidence: 99%

An observational study on the effect of premature ventricular complex burden on long-term outcome

et al. 2017

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The long-term clinical impact of premature ventricular complexes (PVCs) on mortality and morbidity has not been fully studied. This study aimed to investigate the association between the burden of PVCs and adverse clinical outcome.A total of 5778 subjects, who were pacemaker-free and ventricular tachycardia-free at baseline, received 24-hour electrocardiography monitoring between January 1, 2002 and December 31, 2004. Clinical event data were retrieved from the Bureau of National Health Insurance of Taiwan. Multivariate Cox hazards regression models and propensity-score matching were applied to assess the association between PVCs and adverse clinical outcome.Average follow-up time was 10�± 1 year. In all, 1403 subjects expired, 1301 subjects were hospitalized in the cardiovascular (CV) ward, 3384 were hospitalized for any reason, and 631 subjects developed new-onset heart failure (HF). The optimal cut-off PVC frequency (12 beats per day) was obtained through receiver operator characteristic curves, with a sensitivity of 58.4% and specificity of 59.8%. Upon multivariate analysis, a PVC frequency >12 beats per day was an independent predictor for all mortality (hazard ratio [HR]: 1.429, 95% confidence interval [CI]: 1.284–1.590), CV hospitalization (HR: 1.127, 95% CI: 1.008–1.260), all-cause hospitalization (HR 1.094, 95% CI: 1.021–1.173), and new-onset HF (HR: 1.411, 95% CI: 1.203–1.655). Subjects with a PVC frequency >12 beats per day had an increased risk of cardiac death attributable to HF and sudden cardiac death. The incidence rates for mortality and HF were significantly increased in cases of raised PVC frequency. Propensity-score matching analysis also echoed the main findings.Increased PVC burden was associated with a higher incidence of all-cause mortality, CV hospitalization, all-cause hospitalization, and new-onset HF which was independent of other clinical risk factors.

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Atrial fibrillation and microRNAs

Cited by 121 publications

References 128 publications

Selection of reference genes is critical for miRNA expression analysis in human cardiac tissue. A focus on atrial fibrillation

Selection of reference genes is critical for miRNA expression analysis in human cardiac tissue. A focus on atrial fibrillation

Adrenergic signaling in heart failure and cardiovascular aging

An observational study on the effect of premature ventricular complex burden on long-term outcome

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