ATR-FTIR spectroscopy for the routine quality control of exosome isolations

Ramos-Garcia, Victoria; Ten-Doménech, Isabel; Moreno-Giménez, Alba; Gormáz, María; Parra-Llorca, Anna; Shephard, Alex P.; Sepúlveda, Pilar; Pérez-Guaita, David; Vento, Máximo; Lendl, Bernhard; Quintás, Guillermo; Kuligowski, Julia

doi:10.1016/j.chemolab.2021.104401

Cited by 14 publications

(2 citation statements)

References 28 publications

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“…This protocol has been applied with promising results to human milk EVs (Figure 6), which play an important role in early life immunity and intestinal development. 95 AFM-IR spectra obtained from immobilized EVs display marker bands (Figure 6, b) in agreement with those obtained from bulk FTIR studies, 101 thus showing the suitability of this approach for the label-free tapping mode AFM-IR analysis of human milk EVs. As demonstrated in these two approaches, AFM-IR can be applied to the nanoscale label-free chemical analysis of individual EVs and thus provide a valuable contribution to EV research.…”

Section: Extracellular Vesicle Characterizationsupporting

confidence: 77%

AFM-IR for Nanoscale Chemical Characterization in Life Sciences: Recent Developments and Future Directions

V. D. dos Santos,

Hondl,

Ramos-Garcia

et al. 2023

ACS Meas. Sci. Au

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Despite the ubiquitous absorption of mid-infrared (IR) radiation by virtually all molecules that belong to the major biomolecules groups (proteins, lipids, carbohydrates, nucleic acids), the application of conventional IR microscopy to the life sciences remained somewhat limited, due to the restrictions on spatial resolution imposed by the diffraction limit (in the order of several micrometers). This issue is addressed by AFM-IR, a scanning probe-based technique that allows for chemical analysis at the nanoscale with resolutions down to 10 nm and thus has the potential to contribute to the investigation of nano and microscale biological processes. In this perspective, in addition to a concise description of the working principles and operating modes of AFM-IR, we present and evaluate the latest key applications of AFM-IR to the life sciences, summarizing what the technique has to offer to this field. Furthermore, we discuss the most relevant current limitations and point out potential future developments and areas for further application for fruitful interdisciplinary collaboration.

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Section: Extracellular Vesicle Characterizationsupporting

confidence: 77%

AFM-IR for Nanoscale Chemical Characterization in Life Sciences: Recent Developments and Future Directions

V. D. dos Santos,

Hondl,

Ramos-Garcia

et al. 2023

ACS Meas. Sci. Au

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“…The method describes specific absorption bands of proteins, lipids, and nucleic acids that may be found in the molecular cargo of EVs [ 197 ]. Moreover, FTIR provides quantitative spectral data and may highlight possible alterations in EVs biochemical composition based on a clinical condition of a patient.…”

Section: Characterization Of Evs Molecular Contentmentioning

confidence: 99%

Recent advances in optical label-free characterization of extracellular vesicles

et al. 2022

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Extracellular vesicles (EVs) are complex biological nanoparticles endogenously secreted by all eukaryotic cells. EVs carry a specific molecular cargo of proteins, lipids, and nucleic acids derived from cells of origin and play a significant role in the physiology and pathology of cells, organs, and organisms. Upon release, they may be found in different body fluids that can be easily accessed via noninvasive methodologies. Due to the unique information encoded in their molecular cargo, they may reflect the state of the parent cell and therefore EVs are recognized as a rich source of biomarkers for early diagnostics involving liquid biopsy. However, body fluids contain a mixture of EVs released by different types of healthy and diseased cells, making the detection of the EVs of interest very challenging. Recent research efforts have been focused on the detection and characterization of diagnostically relevant subpopulations of EVs, with emphasis on label-free methods that simplify sample preparation and are free of interfering signals. Therefore, in this paper, we review the recent progress of the label-free optical methods employed for the detection, counting, and morphological and chemical characterization of EVs. We will first briefly discuss the biology and functions of EVs, and then introduce different optical label-free techniques for rapid, precise, and nondestructive characterization of EVs such as nanoparticle tracking analysis, dynamic light scattering, atomic force microscopy, surface plasmon resonance spectroscopy, Raman spectroscopy, and SERS spectroscopy. In the end, we will discuss their applications in the detection of neurodegenerative diseases and cancer and provide an outlook on the future impact and challenges of these technologies to the field of liquid biopsy via EVs.

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Quantitative Analysis, Infrared

Cadet¹,

Guárdia

2022

Encyclopedia of Analytical Chemistry

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In this article, after reviewing the different mathematical methods used in quantification by IR, the main advantages and drawbacks and applications with or without previous sample treatment are presented. The bases of the use of quantitative IR in food analysis such as lipids (measurement of unsaturation degrees and lipids determination), carbohydrates, and proteins (secondary structures and quantitative analysis); original examples of the use of IR spectroscopy combined with enzymes; and modern applications in the clinical field are presented in detail, followed by examples of Fourier transform infrared (FTIR) analysis coupled with other analytical methods in different fields such as pharmaceuticals, petroleum, paints, and other industrial products and in the fields of health, environment, and trace compounds analysis in order to think about the limitations and perspectives of IR spectroscopy for quantitative analysis. It appears that the most significant developments in the field of quantitative analysis will probably come through the progress in chemometrics and instrumentation. More recently, approaches combining infrared spectroscopy and deep learning (DL) have shown great potential.

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ATR-FTIR spectroscopy for the routine quality control of exosome isolations

Cited by 14 publications

References 28 publications

AFM-IR for Nanoscale Chemical Characterization in Life Sciences: Recent Developments and Future Directions

AFM-IR for Nanoscale Chemical Characterization in Life Sciences: Recent Developments and Future Directions

Recent advances in optical label-free characterization of extracellular vesicles

Quantitative Analysis, Infrared

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