AtQQS orphan gene and NtNF-YC4 boost protein accumulation and pest resistance in tobacco (Nicotiana tabacum)

Tanvir, Rezwan; Ping, Wenli; Sun, Jialiang; Cain, Morgan; Li, Xuejun; Li, Ling

doi:10.1016/j.plantsci.2022.111198

Cited by 13 publications

(20 citation statements)

References 76 publications

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“…AtNF-YC4 is a key element of the NF-Y transcription factor and contains histone-fold domains, which is universal among plant species with a highly conserved peptide sequence Qi et al, 2019). AtQQS and AtNF-YC4 expression increased total protein content and reduced starch content in both leaf and seed of soybean, rice, and maize, except starch was not detected in the soybean seed (Tanvir et al, 2022). The interaction of an orphan gene from one species with the metabolic network of another species via conserved proteins point to novel possible avenues for understanding the complex traits modulation and phenotypic changes in crop species (O'Conner et al, 2018).…”

Section: Role Of Orphan Genes In Primary Substance Metabolism and Res...mentioning

confidence: 98%

“…Likewise, increased resistance/reduced susceptibility toward soybean cyst nematodes, viruses, aphids, fungi, and viruses can also be attributed to the over-expression of these genes in Arabidopsis and soybean. Furthermore, by mediating crosstalk between primary metabolism and environmental alterations, the QQS gene has also been involved in the optimization and tolerance to biotic/abiotic perturbations (Arendsee et al, 2014;Qi et al, 2019;Tanvir et al, 2022). Recently, 51 OGs were proven to be associated with environmental adaptation in watermelon (Ma et al, 2022).…”

Section: Response To Stressmentioning

confidence: 99%

“…OGs are important for viability to the plants, and the discovery of many protein-protein interaction models has gradually uncovered the mysterious functions of OGs, such as the interaction of AtQQS and AtNF-YC4. OGs were not merely results of random evolutionary development, they may indeed be an essential part of organisms' key trades (Tanvir et al, 2022), and the mysterious function of OGs still needs comprehensive exploration.…”

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

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Research Advances and Prospects of Orphan Genes in Plants

Jiang

Dong

et al. 2022

Front. Plant Sci.

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Orphan genes (OGs) are defined as genes having no sequence similarity with genes present in other lineages. OGs have been regarded to play a key role in the development of lineage-specific adaptations and can also serve as a constant source of evolutionary novelty. These genes have often been found related to various stress responses, species-specific traits, special expression regulation, and also participate in primary substance metabolism. The advancement in sequencing tools and genome analysis methods has made the identification and characterization of OGs comparatively easier. In the study of OG functions in plants, significant progress has been made. We review recent advances in the fast evolving characteristics, expression modulation, and functional analysis of OGs with a focus on their role in plant biology. We also emphasize current challenges, adoptable strategies and discuss possible future directions of functional study of OGs.

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Section: Role Of Orphan Genes In Primary Substance Metabolism and Res...mentioning

confidence: 98%

Section: Response To Stressmentioning

confidence: 99%

Section: Concluding Remarks and Future Perspectivesmentioning

confidence: 99%

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Research Advances and Prospects of Orphan Genes in Plants

Jiang

Dong

et al. 2022

Front. Plant Sci.

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“…As OGs do not contain recognizable domains, functional motifs, or folding patterns, their functions are often unclear such that detailed functional characterization is ultimately necessary. Several studies have successfully demonstrated the diverse roles played by specific OGs as mediators of metabolite synthesis (Li et al, 2009;Li and Wurtele, 2015;Li et al, 2015b;O'Conner et al, 2018;Jiang et al, 2020b;Fang et al, 2021;Shen et al, 2021;Tanvir et al, 2022b), biotic stresses response (Jiang et al, 2018;Qi et al, 2019;Brennan et al, 2020;Jiang et al, 2020a;Wang et al, 2021;Moon et al, 2022;Tanvir et al, 2022a), abiotic stresses response (Yadeta et al, 2014;Li et al, 2019;Ma et al, 2020), species-specific traits, or the regulation of growth and development (Chen et al, 2017;Ni et al, 2017;Wang et al, 2017;Zhao et al, 2018;Dossa et al, 2021). These characteristics make OGs important targets for plant breeding efforts aimed at enhancing specific traits of interest to improve plant stress resistance and quality.…”

Section: Introductionmentioning

confidence: 99%

Brassica rapa orphan gene BR1 delays flowering time in Arabidopsis

et al. 2023

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Orphan genes are essential to the emergence of species-specific traits and the process of evolution, lacking sequence similarity to any other identified genes. As they lack recognizable domains or functional motifs, however, efforts to characterize these orphan genes are often difficult. Flowering is a key trait in Brassica rapa, as premature bolting can have a pronounced adverse impact on plant quality and yield. Bolting resistance-related orphan genes, however, have yet to be characterized. In this study, an orphan gene designated BOLTING RESISTANCE 1 (BR1) was identified and found through gene structural variation analyses to be more highly conserved in Chinese cabbage than in other available accessions. The expression of BR1 was increased in bolting resistant Chinese cabbage and decreased in bolting non-resistant type, and the expression of some mark genes were consist with bolting resistance phenotype. BR1 is primarily expressed in leaves at the vegetative growth stage, and the highest BR1 expression levels during the flowering stage were observed in the flower buds and silique as compared to other tissue types. The overexpression of BR1 in Arabidopsis was associated with enhanced bolting resistance under long day (LD) conditions, with these transgenic plants exhibiting significant decreases in stem height, rosette radius, and chlorophyll content. Transcriptomic sequencing of WT and BR1OE plants showed the association of BR1 with other bolting resistance genes. Transcriptomic sequencing and qPCR revealed that six flowering integrator genes and one chlorophyll biosynthesis-related gene were downregulated following BR1 overexpression. Six key genes in photoperiodic flowering pathway exhibited downward expression trends in BR1OE plants, while the expression of floral repressor AtFLC gene was upregulated. The transcripts of these key genes were consistent with observed phenotypes in BR1OE plants, and the results indicated that BR1 may function through vernalization and photoperiodic pathway. Instead, the protein encoded by BR1 gene was subsequently found to localize to the nucleus. Taken together, we first propose that orphan gene BR1 functions as a novel regulator of flowering time, and these results suggested that BR1 may represent a promising candidate gene to support the selective breeding of Chinese cabbage cultivars with enhanced bolting resistance.

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“…However, plants are not defenceless; they are equipped with a battery of multiple mechanisms (from molecular through biochemical-physiological to structural) that are activated by them to ensure further growth and development and the production of diasporas ( Muszyńska and Labudda, 2019 ; Formela-Luboińska et al., 2020 ; Tokarz et al., 2020 ; Tokarz et al., 2021 ; Fidler et al., 2022 ; Miernicka et al., 2022 ). Among these mechanisms, the control of proteolysis and thereby the quality and composition of proteins and pool of amino acids are of fundamental significance ( Muszyńska et al., 2019 ; Labudda et al., 2020 ; O’Conner et al., 2021 ; Pan et al., 2021 ; Szewińska et al., 2021 ; Tan et al., 2021 ; Sun et al., 2022 ; Tanvir et al., 2022 ; Xing et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%