AtPME17 is a functional <i>Arabidopsis thaliana</i> pectin methylesterase regulated by its PRO region that triggers PME activity in the resistance to <i>Botrytis cinerea</i>

Corpo, Daniele Del; Fullone, Maria Rosaria; Miele, Rossella; Lafond, Mickaël; Pontiggia, Daniela; Grisel, Sacha; Kieffer‐Jaquinod, Sylvie; Giardina, Thierry; Bellincampi, Daniela; Lionetti, Vincenzo

doi:10.1111/mpp.13002

Cited by 51 publications

(59 citation statements)

References 75 publications

(110 reference statements)

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“…Several studies show how plants modify their pectin methylation degree or substitution pattern in order to avoid microbe colonization [ 78 , 81 ]. It is well-described that HG demethylation, as it is necessary to establish links between HG chains through Ca 2+ bonds to form the denominated egg-box complexes [ 82 ], participate in biotic resistance [ 30 , 31 ]. This type of link could explain the M w increase observed for pectic fractions ( Figure 7 ) [ 83 , 84 ].…”

Section: Discussionmentioning

confidence: 99%

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Immune Priming Triggers Cell Wall Remodeling and Increased Resistance to Halo Blight Disease in Common Bean

Rubia

Mélida

Centeno

et al. 2021

Plants

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The cell wall (CW) is a dynamic structure extensively remodeled during plant growth and under stress conditions, however little is known about its roles during the immune system priming, especially in crops. In order to shed light on such a process, we used the Phaseolus vulgaris-Pseudomonas syringae (Pph) pathosystem and the immune priming capacity of 2,6-dichloroisonicotinic acid (INA). In the first instance we confirmed that INA-pretreated plants were more resistant to Pph, which was in line with the enhanced production of H2O2 of the primed plants after elicitation with the peptide flg22. Thereafter, CWs from plants subjected to the different treatments (non- or Pph-inoculated on non- or INA-pretreated plants) were isolated to study their composition and properties. As a result, the Pph inoculation modified the bean CW to some extent, mostly the pectic component, but the CW was as vulnerable to enzymatic hydrolysis as in the case of non-inoculated plants. By contrast, the INA priming triggered a pronounced CW remodeling, both on the cellulosic and non-cellulosic polysaccharides, and CW proteins, which resulted in a CW that was more resistant to enzymatic hydrolysis. In conclusion, the increased bean resistance against Pph produced by INA priming can be explained, at least partially, by a drastic CW remodeling.

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Section: Discussionmentioning

confidence: 99%

“…macuolicola in Arabidopsis, which leads to an increment of PME activity [ 27 ]. Thus, pme17 Arabidopsis mutants showed an increase in susceptibility to B. cinerea [ 30 ]. In the same way, PME inhibitor (PMEI) mutants such as pmei10 , pmei11 , and pmei12 lead to immunity against B. cinerea [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Immune Priming Triggers Cell Wall Remodeling and Increased Resistance to Halo Blight Disease in Common Bean

Rubia

Mélida

Centeno

et al. 2021

Plants

View full text Add to dashboard Cite

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“…The demethylesterification of pectin, controlled by PMEs, is considered to affect the porosity of the cell wall and, thus, exposes the plant to an easier degradation by pathogen enzymes (Raiola et al 2011). However, PME activity has been also associated with the activation of plant immunity and resistance against pathogens (Del Corpo et al 2020). In a recent study, in contrast to P. radiata, the resistant species P. pinea infected by F. circinatum showed a high induction of pectin methylesterase inhibitor (PMEI) genes and an inhibition of PMEs (Zamora-Ballesteros et al 2021).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification and Characterization of Fusarium circinatum-Responsive lncRNAs in Pinus radiata

Zamora-Ballesteros

Martín‐García

Suárez-Vega

2021

Preprint

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One of the most promising strategies of Pine Pitch Canker (PPC) management is the use of reproductive plant material resistant to the disease. Understanding the complexity of plant transcriptome that underlies the defence to the causal agent Fusarium circinatum, would greatly facilitate the development of an accurate breeding program. Long non-coding RNAs (lncRNAs) are emerging as important transcriptional regulators under biotic stresses in plants. However, to date, characterization of lncRNAs in conifer trees has not been reported. In this study, transcriptomic identification of lncRNAs was carried out using strand-specific paired-end RNA sequencing, from Pinus radiata samples inoculated with F. circinatum at an early stage of infection. Overall, 13,312 lncRNAs were predicted through a bioinformatics approach, including long intergenic non-coding RNAs (92.3%), antisense lncRNAs (3.3%) and intronic lncRNAs (2.9%). Compared with protein-coding RNAs, pine lncRNAs are shorter, have lower expression, lower GC content and harbour fewer and shorter exons. A total of 164 differentially expressed (DE) lncRNAs were identified in response to F. circinatum infection in the inoculated versus mock-inoculated P. radiata seedlings. The predicted cis-regulated target genes of these pathogen-responsive lncRNAs were related to defence mechanisms such as kinase activity, phytohormone regulation, and cell wall reinforcement. Co-expression network analysis of DE lncRNAs, DE protein-coding RNAs and lncRNA target genes also indicated a potential network regulating pectinesterase activity and cell wall remodelling. This study presents the first analysis of conifer lncRNAs involved in the regulation of defence network and provides the basis for future functional characterizations of lncRNAs in relation to pine defence responses against F. circinatum.

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“…Pectin is one of the main components of the cell wall [45] and is usually secreted from the Golgi apparatus into the cell wall in highly methylated forms [46] and then undergoes demethylation by PME, consequently increasing metal ion binding sites in the cell wall [47]. On the other hand, soil bicarbonate can increase the apoplast pH of leaves, and the activity of PME increases as the pH increases, thereby enhancing iron precipitation in the apoplast and reducing its bioavailability [48,49]. In this study, NCFe could signi cantly reduce the expression of PbPME due to sulfonic acid groups on NCFe and effectively reduce the apoplast pH.…”

Section: Regulation Of Relative Gene Expression In Pear Leavesmentioning

confidence: 99%

Remediation of pear iron chlorosis by nanocellulose-iron chelation and mechanisms

Wang¹

2021

Preprint

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Background: Nanocrystal cellulose has a strong ability to chelate iron and the nanocomposite possesses strong adsorptive property. Iron deficiency chlorosis (IDC) is a mineral disorder that weakens pear photosynthesis and cause a significant decline in plant yield and quality. Conventional methods to control IDC are generally due to low efficiency and overuse of chemicals. The purpose of this study was to explore the capability of nanocellulose (NC)-Fe chelate to remediate pear IDC. Acidic hydrolyzed NCs were chelated with Fe (NCFe) based on the net charge density of the components. Foliar application of NCFe was employed to pre-etiolated seedlings of Pyrus betulifolia as a plant material. The ability of NCFe to promote active iron content (CFe), photosynthesis rate, and relative gene expression was studied. Results: Nanocrystal cellulose prepared by acidic hydrolysis exhibit rod-like whiskers carrying on negative charges. When NCs were mixed with FeSO4, the NCFe particles maintained a small, whisker-like morphology with small dots (Fe) on the surface of the NC particles. The Z-average hydrodynamic diameter and zeta potential of the NC whiskers measured by DLS were 84.3 ± 0.2 nm and -47.3 ± 1.7 mV, respectively. The particle size and zeta potential of NCFe were 107.4±3.0 nm and -9.7±0.4 mV, respectively. The results showed that NCFe could significantly mitigate IDC in seedlings by increasing CFe, photosynthesis parameters, major physiological indicators, and regulating the expression of key enzymes. When NCFe was prepared at a NC-to-Fe charge density ratio of 1:3,000, CFe and chlorophyll contents were enhanced by approximately 9 times and 72.7%, respectively; the major physiological indicators were all significantly increased. Interestingly, NCFe treatment significantly downregulated the expression of the pectin methylesterase gene (PbPME) and upregulated the expression of the ferritin gene (PbFER) to increase CFe.Conclusion: NCs have strong potential to promote plant photosynthesis when chelated with Fe. The remediation capability of NCFe to IDC is attributed to the enhancement of photosynthesis parameters and indicators. NCFe treatment significantly downregulated the expression of the PME gene (PbPME) and upregulated the expression of the ferritin gene (PbFER) to increase the active iron content. This finding will provide a good alternative and a complementary strategy for Fe-chelate applications in plant iron chlorosis management.

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AtPME17 is a functional Arabidopsis thaliana pectin methylesterase regulated by its PRO region that triggers PME activity in the resistance to Botrytis cinerea

Cited by 51 publications

References 75 publications

Immune Priming Triggers Cell Wall Remodeling and Increased Resistance to Halo Blight Disease in Common Bean

Immune Priming Triggers Cell Wall Remodeling and Increased Resistance to Halo Blight Disease in Common Bean

Genome-Wide Identification and Characterization of Fusarium circinatum-Responsive lncRNAs in Pinus radiata

Remediation of pear iron chlorosis by nanocellulose-iron chelation and mechanisms

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