ATPase activity of Plasmodium falciparum MLH is inhibited by DNA-interacting ligands and dsRNAs of MLH along with UvrD curtail malaria parasite growth

Tarique, Mohammed; Chauhan, Manish; Tuteja, Renu

doi:10.1007/s00709-016-1021-8

Cited by 6 publications

(4 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of PfPSH2 (PF3D7_120200) in the list of non-mutable genes further strengthens our observation that PfPSH2 might be crucial for the survival of parasite. Similar studies were reported previously where PfUvrD dsRNA treatment to the parasite culture lead to growth defects and morphological abnormalities 58 . The semi quantitative real time PCR results suggest that the mRNA expression level of PfPSH2 gene is reduced in PfPSH2 dsRNA treated culture.…”

Section: Discussionsupporting

confidence: 89%

Plasmodium falciparum specific helicase 2 is a dual, bipolar helicase and is crucial for parasite growth

Chauhan

Tuteja

2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

Human malaria infection is a major challenge across the globe and is responsible for millions of deaths annually. Rapidly emerging drug resistant strains against the new class of anti-malarial drugs are major threat to control the disease burden worldwide. Helicases are present in every organism and have important role in various nucleic acid metabolic processes. Previously we have reported the presence of three parasite specific helicases (PSH) in Plasmodium falciparum 3D7 strain. Here we present the detailed biochemical characterization of PfPSH2. PfPSH2 is DNA and RNA stimulated ATPase and is able to unwind partially duplex DNA and RNA substrates. It can translocate in both 3′ to 5′ and 5′ to 3′ directions. PfPSH2 is expressed in all the stages of intraerythrocytic development and it is localized in cytoplasm in P. falciparum 3D7 strain. The dsRNA mediated inhibition study suggests that PfPSH2 is important for the growth and survival of the parasite. This study presents the detailed characterization of PfPSH2 and lays the foundation for future development of PfPSH2 as drug target.

show abstract

Section: Discussionsupporting

confidence: 89%

Plasmodium falciparum specific helicase 2 is a dual, bipolar helicase and is crucial for parasite growth

Chauhan

Tuteja

2019

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…5,7a,12 Our results corroborate with the results of other studies that have also highlighted the inhibitory effects of several commercially available compounds. 11,13 Other studies have revealed that inhibition of methyltransferases has a significant effect on the dengue virus infection. 13,14 In order to validate the differences between eight different concentrations, we employed the ANOVA test.…”

Section: Effect Of Various Compounds On Mtase Activitymentioning

confidence: 99%

“…Other authors such as Yalcin and Bayraktar also reported time-dependent inactivation of methyltransferase as the degree of inhibition increased with time. [11][12][13]15,16 CONCLUSION These outcomes are significant as a beginning stage for further illustration of the specific components of the antiviral movement and to pick up the expected information to additionally grow new, increasingly intense and safe medications to decrease the diseases brought about by this pertinent pathogen. The two compounds were found as the most potent inhibitors among the five different screened compounds.…”

Section: Time-dependent Inhibitionmentioning

confidence: 99%

Pharmacological Modulation of Flavivirus Methyltransferase Activity of Dengue Virus (DEV)

Alwabli¹,

Alattas²,

Alhebshi³

et al. 2019

IJPER

View full text Add to dashboard Cite

Background:The occurrence of dengue fever plagues has expanded significantly in the course of the most recent couple of decades. Be that as it may, no immunization or antiviral treatments are accessible. In this manner, the requirement for protected and successful antiviral medications have turned out to be essential. Aim: Methyltransferases, with their altered enzymatic action, have been found associated with several diseases or infections. It has been observed that the dengue virus infection is correlated with the hyperactivity of methyltransferase. In the current study, we proposed to investigate the inhibitory effects of commercially available compounds on the methyltransferase activity. Materials and Methods: Evaluation of methyltransferase activity of the purified enzyme and their inhibitors was carried out using Methyltransferase Activity Assay Kit (Colorimetric). Results: Among the screened components, we identify two effective compounds that revealed significant effect against flavivirus methyltransferase activity. Further, time-dependent inhibition profiles for both compounds revealed that preincubation of fractions of Azacitidine and Zebularine for different time windows showed time-dependent inactivation of methyltransferase as the degree of inhibition increased with time. Conclusion: We conclude that both Azacitidine and Zebularine may be emerging as possible suitable drugs for the treatment of dengue virus infection. Cite this article: Alwabli AS, Alattas SG, Alhebshi AM, Zabermawi NM, Kaneez F, Azhar EI, Alkenani N, Al-ghmady K, Qadri I. Pharmacological Modulation of Flavivirus Methyltransferase Activity of Dengue Virus (DEV). Indian J of Pharmaceutical Education and Research. 2019;53(3 Suppl 2):s432-s436. PICTORIAL ABSTRACT SUMMARYThis investigation, clearly shows that Azacitidine and Zebularine could be an elective second, possible, of a drug in the treatment of Dengue (DENV) infection.

show abstract

“…5,6 In addition to the RecA domains, the DHX8 helicase domain contains Winged-Helix (WH), ratchet-like, and Oligonucleotide Binding (OB)-fold domains at the C-terminus. [7][8][9][10] Several studies have been published on the activity and role of normal DHX8 as well as on its mutated forms. [11][12][13] These studies have also highlighted its association with important biological processes such as hematopoiesis and are linked to certain diseases.…”

Section: Introductionmentioning

confidence: 99%

Binding of Novobiocin Paves the Way for Inhibition of DEAH-box Helicase 8

Ajmal¹,

Almutairi²,

Ullah³

et al. 2023

Ind. J. Pharm. Edu. Res

View full text Add to dashboard Cite

Introduction: DEAH-box helicase 8 (DHX8) is a crucial DEAH-box RNA helicase involved in splicing and required for the release of mature mRNA from the splice. Here, we report the interaction study of human DHX8 and four test compounds namely etoposide, netropsin, nogalamycin, and novobiocin. Materials and Methods: Molecular docking and fluorescence emission spectra techniques were employed to determine the binding and inhibitory effect of test compounds. Results: Our docking and fluorescence emission spectra results showed that DHX8 has a good binding preference for novobiocin among these four test compounds. Moreover, fluorescence emission spectra of DHX8 with novobiocin also revealed the 2.5 µM concentration is an effective novobiocin concentration to inhibit the activity of DHX8. Conclusion: These findings provide an in-depth understanding of the inhibition of DHX8 and contribute insights towards the development of novobiocin as a therapeutic molecule against the DHX8 in targeted diseases.

show abstract

ATPase activity of Plasmodium falciparum MLH is inhibited by DNA-interacting ligands and dsRNAs of MLH along with UvrD curtail malaria parasite growth

Cited by 6 publications

References 43 publications

Plasmodium falciparum specific helicase 2 is a dual, bipolar helicase and is crucial for parasite growth

Plasmodium falciparum specific helicase 2 is a dual, bipolar helicase and is crucial for parasite growth

Pharmacological Modulation of Flavivirus Methyltransferase Activity of Dengue Virus (DEV)

Binding of Novobiocin Paves the Way for Inhibition of DEAH-box Helicase 8

Contact Info

Product

Resources

About