ATPase activity associated with vacuoles and tonoplast vesicles isolated from the CAM plant, <i>Kalanchoë daigremontiana</i>

Aoki, Kunio; Nishida, Kojiro

doi:10.1111/j.1399-3054.1984.tb04243.x

Cited by 43 publications

(11 citation statements)

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“…These results suggest that pineapple can maintain maximum tonoplast LIN0 3 --ATPase activity at a wide temperature range. The optimum temperature of the enzyme in the three CAM species was basically in agreement with the temperatures of 35 to 38"C at which tonoplast ATPase activity is usually determined (Aoki and Nishida, 1984;Jochem et al, 1984;Jochem and Luttge, 1987;Struve and Luttge, 1987;White and Smith, 1989;Warren et al, 1992). 0 ' Neill et al (1983) studied the effect of temperature on corn root tonoplast .dN0 3 --ATPase activities, in which a linear temperature gradient was used between 5 to 50°C at 5°C intervals, and found that the tonoplast .dN0 3 --ATPase activity increased to a maximum at 45oC, then abruptly declined at 50°C.…”

Section: A Tpase and Ppase Activitiessupporting

confidence: 79%

“…LIN0 3 --ATPase activity was assayed as shown in "Materials and Methods". The temperature for determining LIN0 3 --ATPase activity was 37"C Aoki and Nishida (1984), Smith et al (1984), and Jochem and Lilttge (1987) (Fig. I).…”

Section: Replicationmentioning

confidence: 96%

“…The optimum pH for ATPase in tonoplasts isolated from other plants is in the range of 6.5 to 8.5 (Walker and Leigh, 1981 ;Aoki and Nishida, 1984 ;Wang and Sze, 1985;Jochem and Llittge, 1987;Ballesteros et al, 1996). Fig.…”

Section: Ph-dependence Of Tonoplast Dn0 3 --Atpase and Ppase Activitiesmentioning

confidence: 99%

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Characteristics of Adenosinetriphosphatase and Inorganic Pyrophosphatase in Tonoplasts Isolated from Three CAM Species,Ananas comosus, Kalanchoë pinnataand K. daigremontiana

Chen

Nose

2000

Plant Production Science

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The characteristics of adenosinetriphosphatase (ATPase) and inorganic pyrophosphatase (PPase) in the tonoplasts isolated from leaf mesophyll homogenates of Ananas comosus (pineapple), a hexose-utilizing species with high PPi-PFK activities, and Kalancho(j pinnata and K daigremontiana, starch-utilizing species with high ATP-PFK activities, were investigated. The ATPase and nitrate-sensitive ATPase (L1N0 3 --ATPase) activities were higher than the PPase activity of pineapple, but the reverse was the case in the two Kalancho£:' species. The optimum pH for L1N0 3 --ATPase in pineapple, K. daigremontiana and K pinnata was 7 .0,....,_.8.25, 7 .5,....,_.8.25 and 7 .5,....,_.8.25, respectively, and that for PPase was 6.5...._,7.5 in all species. The Km of L1N0 3 --ATPase for Na 2 -ATP for pineapple, K daigremontiana and K pinnata was 0.47, 0.48 and 0.43 mM, respectively, and V max was 52.6, 27.0 and 40.0 ,umol Pi mg-1 protein h-1 , respectively.The optimum MgS0 4 and Na 4 PP 1 concentrations for the PPase activity of the three CAM species were approximately 2 mM and 0.16 mM, respectively. The optimum temperature for the L1N0 3 --ATPase in pineapple, K daigremontiana and K. pinnata was 35----43, 35,...,.,.,40 and 37°C, respectively, and that for PPase was 46,....,_.49°C. In addition, at a high temperature, the decrement of tonoplast L1N0 3 --ATPase and PPase activities in pineapple was less than that in the two Kalanchoe· species. Thus, pineapple obviously maintained high tonoplast L1N0 3 --ATPase and PPase activities at high temperatures.

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Section: A Tpase and Ppase Activitiessupporting

confidence: 79%

Section: Replicationmentioning

confidence: 96%

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Characteristics of Adenosinetriphosphatase and Inorganic Pyrophosphatase in Tonoplasts Isolated from Three CAM Species,Ananas comosus, Kalanchoë pinnataand K. daigremontiana

Chen

Nose

2000

Plant Production Science

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“…daigremontiana (4). The relationship between this permease, ATPase activity known to be present on tonoplast vesicles (2,23), and malate transport, however, remains to be determined.…”

mentioning

confidence: 99%

Electrophoretic Analysis of Protoplast, Vacuole, and Tonoplast Vesicle Proteins in Crassulacean Acid Metabolism Plants

Kenyon¹,

Black²

1986

Plant Physiol.

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ABSTRACrProtoplasts and vacuoles were isolated and purified in large numbers from the CAM plants Ananas comosus (pineapple) and Sedum telephium for protein characterization. Vacuoles were further fractionated to yield a tonoplast vesicle preparation. Polypeptides of protoplasts, vacuoles, and tonoplast vesicles were compared to whole leaf polypeptides from both plants by one-dimensional sodium dodecylsulfate-polyacrylamide gel electrophoresis. Approximately 100 vacuole polypeptides could be resolved of which 25 to 30% were enriched in the tonoplast vesicles. The proteins of protoplasts, vacuoles, and tonoplast vesicles from A. comosus were analyzed further by two-dimensional gel electrophoresis. When onedimensional electrophoretograms of A. comosus polypeptides were stained with a glycoprotein-specific periodic acid Schiff stain, very few polypeptides appeared to be glycosylated, whereas a large number of glycosylated polypeptides were detected with a silver-based glycoprotein stain particularly in tonoplast vesicles. Analysis of the enzymic content of vacuoles from both plants indicated the presence of a variety of hydrolases, including bromelain as a major constituent of A. comosus. No substrate-specific ATPase, however, could be detected in vacuoles or tonoplast vesicles from either plant. lutoids and sugar uptake into vacuoles from beet root and pea mesophyll also have been shown (8,15). The association of ATPase and permeases with the tonoplast suggests that this membrane may have a more complex composition than previously thought. The polypeptide composition ofthe tonoplast has been examined in other plant species, e.g. beet root (16) and Hippeastrum petals (27), but not in CAM plants.The vacuoles of CAM plants are unique because they are directly involved in photosynthetic carbon assimilation (9-1 1). CAM leaf vacuoles participate in the nocturnal accumulation and diurnal release of large amounts of malic acid (10) and in pineapple hexoses and malate fluctuate reciprocally each day (9). During the night malic acid accumulates against a steep concentration gradient implying that malic acid is actively transported into vacuoles. Evidence is available for a malate permease on the tonoplast ofK. daigremontiana (4). The relationship between this permease, ATPase activity known to be present on tonoplast vesicles (2, 23), and malate transport, however, remains to be determined.Characterization of the protein composition of the vacuole and tonoplast is an initial step in understanding the processes by which the vacuole functions as an active metabolic compartment within the plant cell. This study was undertaken to characterize the proteins and polypeptides found in tonoplast vesicles and vacuoles from two CAM plants.The vacuole in higher plant cells is a multifunctional organelle which often may occupy more than 90% of the mature cell volume. It is involved in osmotic regulation of cell volume, storage of ions and metabolites, compartmentation of specific enzymes including hydrolases, sequestration ofsecondary m...

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“…Solubilization (20) and 'negative purification' (7) of both ATPases have been attempted which will help to further elucidate their reaction mechanism. Meanwhile, comparatively little efforts were undertaken to characterize the substrate kinetics (1,9) of the tonoplast-type ATP-dependent H+ transport although a kinetic regulation ofthe H+ transport ATPases seemed likely (4).…”

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confidence: 99%

ADP Is a Competitive Inhibitor of ATP-Dependent H⁺ Transport in Microsomal Membranes from Zea mays L. Coleoptiles

Rausch¹,

Ziemann-Roth²,

Hilgenberg³

1985

Plant Physiol.

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MATERIALS AND METHODSAn anion-sensitive ATP-dependent H transport in microsomal membranes from Zea mays L. coleoptiles was partially characterized using the pH gradient-dependent decrease of unprotonated neutral red. The following criteria strongly suggest a tonoplast origin of Recently, evidence has accumulated for the presence of two microsomal H+-ATPases in plants involved in the production of electrogenic H+ gradients (4,6,8,10, 11,18,19). One is associated with high density membranes (about 1.16-1.17 g/cm3 inhibited by vanadate and correlated with marker enzymes for plasma membrane (4,7,8,19). The second one is found in low density membranes (about 1.10 g/cm3), activated by Cl-, inhibited by NO3 and is thought to be of tonoplast origin (4,9,16,17,19). This latter assumption is strongly supported by recent results obtained with tonoplast vesicles prepared from previously isolated vacuoles (4) which show the same characteristics. Solubilization (20) and 'negative purification' (7) of both ATPases have been attempted which will help to further elucidate their reaction mechanism. Meanwhile, comparatively little efforts were undertaken to characterize the substrate kinetics (1, 9) of the tonoplast-type ATP-dependent H+ transport although a kinetic regulation ofthe H+ transport ATPases seemed likely (4).In this study, we partially characterize a tonoplast-type ATPdependent H+ transport in microsomal membranes (4,25). The disappearence of unprotonated neutral red in the reaction medium which reflects the accumulation of the protonated species in the membrane vesicles (14) is used to monitor H+ transport. As free protonated neutral red is in equilibrium with membranebound protonated neutral red with different spectral characteristics (13), it is not opportune to follow the accumulation of the protonated species. We present evidence for a competitive inhibition ofATP-dependent H+ transport by ADP By that time, the coleoptiles had an average length of 4 cm. The coleoptiles were cut off in room light with a razor blade 2 mm from the node, and the primary leaves were removed. After collection on ice cold deionized H20, they were filtered and blotted dry on filter paper. After determination of fresh weight, they were immediately immersed into the medium for plasmolysis (20 mM Mops' 400 mm sucrose, adjusted to pH 7.2 with 0

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ATPase activity associated with vacuoles and tonoplast vesicles isolated from the CAM plant, Kalanchoë daigremontiana

Cited by 43 publications

References 20 publications

Characteristics of Adenosinetriphosphatase and Inorganic Pyrophosphatase in Tonoplasts Isolated from Three CAM Species,Ananas comosus, Kalanchoë pinnataand K. daigremontiana

Characteristics of Adenosinetriphosphatase and Inorganic Pyrophosphatase in Tonoplasts Isolated from Three CAM Species,Ananas comosus, Kalanchoë pinnataand K. daigremontiana

Electrophoretic Analysis of Protoplast, Vacuole, and Tonoplast Vesicle Proteins in Crassulacean Acid Metabolism Plants

ADP Is a Competitive Inhibitor of ATP-Dependent H⁺ Transport in Microsomal Membranes from Zea mays L. Coleoptiles

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ATPase activity associated with vacuoles and tonoplast vesicles isolated from the CAM plant, Kalanchoë daigremontiana

Cited by 43 publications

References 20 publications

Characteristics of Adenosinetriphosphatase and Inorganic Pyrophosphatase in Tonoplasts Isolated from Three CAM Species,Ananas comosus, Kalanchoë pinnataand K. daigremontiana

Characteristics of Adenosinetriphosphatase and Inorganic Pyrophosphatase in Tonoplasts Isolated from Three CAM Species,Ananas comosus, Kalanchoë pinnataand K. daigremontiana

Electrophoretic Analysis of Protoplast, Vacuole, and Tonoplast Vesicle Proteins in Crassulacean Acid Metabolism Plants

ADP Is a Competitive Inhibitor of ATP-Dependent H+ Transport in Microsomal Membranes from Zea mays L. Coleoptiles

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ADP Is a Competitive Inhibitor of ATP-Dependent H⁺ Transport in Microsomal Membranes from Zea mays L. Coleoptiles