ATP measurements on single living cells: a dynamic approach

Bernengo, Jean‐Claude; Brau, Frédéric; Steghens, Jean Paul

doi:10.1109/iembs.1996.646314

Cited by 1 publication

(1 citation statement)

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“…For biological analysis applications, ATP concentrations at the low-end of single-cell limits (up to 1 M) have been demonstrated using firefly luciferase on a fiber-optic sensing platform in noncellular experiments [88]. A very limited number of efforts have successfully demonstrated the detection of ATP in single cells using the chemiluminescence of the luciferin/luciferase couple in mouse myoblasts (0.01-1 mM concentration range) [10] and myoballs from Wistar rats (0.01-1 mM concentration range) [9]. Similarly, chemiluminescence has been used to measure bacteria in water using a luciferine/luciferase combination; however, this process requires lysing (breaking up) the bacteria prior to successful introduction of the chemiluminescent agent [89].…”

Section: A Luminescencementioning

confidence: 98%

Sensor Technologies for Monitoring Metabolic Activity in Single Cells—Part I: Optical Methods

2004

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A review of optical, chemical, and biological sensors to detect metabolic activity at the single-cell level is presented in the context of the development of lab-on-a-chip research instrumentation. The sensors reviewed include optical sensors, at both research and commercial levels, that can optically detect intracellular metabolites including adenosine triphosphate, nicotinamide-adenine dinucleotide, reduced flavin adenine dinucleotide, and other metabolites, including oxygen, carbon dioxide, and glucose. Methods to optically detect pH changes which are a general indicator of activity in extracellular space are also briefly reviewed. Performance metrics such as sensitivity, sensor size, drift, time response, and sensing range are included when available. Highly suitable optical sensor technologies for monitoring cellular metabolic activity include luminescent (fluorescent, phosphorescent, and chemiluminescent) and colorimetric optical probes. Different approaches to extracting luminescent and colorimetric information are reviewed, including benchtop techniques, fiber-optic approaches, and the use of probes encapsulated by biologically localized embedding. A brief discussion of alternate optical sensor technologies, such as surface plasmon resonance and infrared absorption spectroscopy, is also presented.

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