ATP-induced mucin release from cultured airway globet cell
involves, in part, activation of phospholipase A2

Shin, CY; Jo, Mi-Jeong; Lee, W. J.; Ryu, Jiho; Kim, K. C.; Ko, KH

doi:10.1358/mf.2001.23.2.627929

Cited by 2 publications

(1 citation statement)

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“…ATP has been shown to stimulate mucin release (secretion) by activation of protein kinase C (PKC) following activation of phospholipase C (PLC) coupled to the P 2 receptor via G-proteins (Ko et al, 1997). Shin and his colleagues investigated pathways downstream to the PKC activation in ATP-induced mucin release from HTSE cells and suggested that PLA 2 is also involved in ATP-induced mucin release and its activation is sequential to the PLC-PKC pathway (Shin et al, 2001). On the other hand, a natural triterpenoid, pachymic acid was reported to inhibit the PLA 2 in prostatic adenocarcinoma cells (Gapter et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

Carbenoxolone and triterpenoids inhibited mucin secretion from airway epithelial cells

Heo

Kim

Lee

et al. 2007

Phytotherapy Research

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This study investigated whether carbenoxolone, oleanolic acid and ursolic acid affect ATP-induced mucin secretion from cultured airway epithelial cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled using (3)H-glucosamine for 24 h and chased for 30 min in the presence of varying concentrations of each agent to assess the effects on (3)H-mucin secretion. The possible cytotoxicity of each agent was investigated with a lactate dehydrogenase assay. The results were as follows: (1) carbenoxolone, oleanolic acid and ursolic acid significantly inhibited the secretion of airway mucin induced by ATP; (2) none of the compounds showed significant cytotoxicity at any concentration. This result suggests that carbenoxolone, oleanolic acid and ursolic acid can regulate 'mucin secretion induced by ATP'--phenomenon simulating mucus overproduction from inflamed airway epithelial cells--y directly acting on airway mucin-secreting cells.

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Section: Resultsmentioning

confidence: 99%