ATP‐induced calcium mobilization and inositol 1,4,5‐trisphosphate formation in H‐35 hepatoma cells

Horstman, Debra A.; Tennes, Karin A.; Putney, James W.

doi:10.1016/0014-5793(86)80809-2

Cited by 45 publications

(18 citation statements)

References 13 publications

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“…These early events include: (i) increase in cytosolic free calcium (7)(8)(9), (ii) formation of inositol phosphates and stimulation of calcium efflux (9,10), (iii) release of arachidonic acid and formation of prostaglandin E2 (PGE2), (iv) formation of diacylglycerol and hydrolysis of phosphatidylcholine, (v) alkalinization of the cytosol, and (vi) activation of uridine uptake (unpublished results). Similar effects of ATP have been described by others in a variety of cell lines (11)(12)(13)(14)(15)(16)(17)(18)(19)(20). Studies of various growth factors suggested that some early events were only regulatory for DNA synthesis while others were required for mitogenesis (for review, see ref.…”

supporting

confidence: 81%

Activation of early events of the mitogenic response by a P2Y purinoceptor with covalently bound 3'-O-(4-benzoyl)-benzoyladenosine 5'-triphosphate.

González

Wang

Huang

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

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3'-O-(4-Benzoyl)benzoyl-ATP (BzATP), a photoaffinity analog of ATP, was used as a ligand for a P2Y purinoceptor (adenine nucleotide receptor) present in intact Swiss 3T3 and 3T6 cells and A-431 epidermoid carcinoma cells. Photolysis of serum-starved cells in the presence of 10-50 microM BzATP, followed by extensive washing to remove unincorporated BzATP, induced the release of arachidonic acid. A trace (less than 0.01%) of photoincorporated BzATP was as effective as when 50 microM BzATP or ATP was contained in the incubation medium during the assay. Photoincorporated BzATP also stimulated the production of prostaglandin E2 and the accumulation of cyclic AMP. In previous studies, we demonstrated that these three events are obligatory early steps in a pathway leading to DNA synthesis in the above cell lines. The evidence indicated that the purinoceptor activated by extracellular ATP or BzATP was linked to a pertussis toxin-sensitive GTP-binding protein. Consistent with these observations, we now find that pertussis toxin inhibits the effect of photoincorporated BzATP on arachidonic acid release. These results indicate that BzATP is an effective agonist for the P2Y purinoceptor concerned with stimulation of DNA synthesis in 3T3, 3T6, and A-431 cells. Furthermore, after photolysis it becomes irreversibly associated with intact cells and promotes the activation of early events required for DNA synthesis.

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supporting

confidence: 81%

Activation of early events of the mitogenic response by a P2Y purinoceptor with covalently bound 3'-O-(4-benzoyl)-benzoyladenosine 5'-triphosphate.

González

Wang

Huang

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

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“…Other ATP receptors have been proposed to alter intracellular calcium in a variety of ways. Phosphoinositide hydrolysis, inositol 1,4,5-phosphate production and increased cytosolic calcium levels result from the addition of micromolar concentrations of ATP to Ehrlich ascites tumor cells [19], H-35 hepatoma cells [20], hepatocytes [21], DDT smooth muscle cells (Dubyak, G.R., personal communication), and rat aortic myocytes [22]. A calcium-permeable channel is activated by extracellular ATP in arterial smooth muscle cells [23].…”

Section: Resultsmentioning

confidence: 99%

Extracellular ATP induces Ca²⁺ transients in cardiac myocytes which are potentiated by norepinephrine

Young

Scarpa

1987

FEBS Letters

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Isolated rat ventricular cardiac myocytes loaded with the fluorescent calcium indicator fura2 Showed significant changes in intracellular calcium concentrations upon exposure to > 1/tM ATP (ECs0 = 7.4_ 1.3 gM, n =4, SE), suggesting that extracellular ATP may have an important influence on myocardial contractility. The response was found to be highly ATP specific and required extracellular calcium. Furthermore, 30 s pretreatment of the cells with 0.2-1 #M norepinephrine decreased the concentration of ATP required for the Ca z ÷ transient, shifting the EC50 for ATP to 1.7 +_ 0.1 #M (n = 3, SE). fl-Propranolol (a ilrreceptor antagonist) prevented potentiation, whereas phentolamine (an ~q-receptor antagonist) did not, indicating that regulation is through the fll-adrenergic receptor. ATP and norepinephrine released locally from sympathetic neurons may act in concert through the ATP and fll-adrenergic receptors to regulate myocardial calcium homeostasis.

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“…In addition, we observed a stimulation of the rate of Na+, K+, and uridine entry, and enhanced ornithine decarboxylase activity (unpublished observations). Other workers have reported that extracellular ATP activates similar early signals in hepatocytes (17)(18)(19)(20), Ehrlich ascites tumor cells (21)(22)(23), mouse macrophages (24), H35 hepatoma cells (25), endothelial cells (26)(27)(28)(29)(30), and turkey erythrocytes (31).…”

mentioning

confidence: 93%

Extracellular ATP is a mitogen for 3T3, 3T6, and A431 cells and acts synergistically with other growth factors.

Huang

Wang

Heppel

1989

Proc. Natl. Acad. Sci. U.S.A.

142

107

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Extracellular ATP in concentrations of 5-50 gLM displayed very little mitogenic activity by itself but it caused synergistic stimulation of [3H]thymidine incorporation in the presence of phorbol 12-tetradecanoate 13-acetate, epidermal growth factor, platelet-derived growth factor, insulin, adenosine, or 5'-(N-ethyl) These findings raise the possibility that exogenous ATP acts as a growth factor, and this was implied in two recent papers (23, 24), where ATP was compared to "more conventional mitogens." However, it is premature to call ATP a mitogen without more evidence than has been available. One must be careful to rule out the possibility that ATP is converted to adenosine by ubiquitous hydrolytic enzymes on the cell surface (ectoenzymes) (32). It was demonstrated some years ago in Rozengurt's laboratory (11-13) and by others (33) that adenosine is a potent mitogen for Swiss 3T3 cells, and this is also true for certain adenosine analogues such as NECA.In this paper, we show that exogenous ATP is a mitogen for 3T3, 3T6, A431, HFF (34), and DDT1-MF2 (35) cells. We confirm that adenosine is also mitogenic, but several lines of evidence show that ATP acts independently, in its own right, and not by being first converted to adenosine by ectoenzymes. MATERIALS AND METHODS Materials. [3H]Thymidine was purchased from Amersham.Culture media and fetal bovine serum (FBS) were from GIBCO. ATP, adenosine, epidermal growth factor (EGF), adenosine 5'-[8,y-imido]triphosphate (AdoPP[NH]P), and all other nucleotides were from Boehringer Mannheim. Bovine insulin, NECA, and phorbol 12-tetradecanoate 13-acetate (PTA) were from Sigma. Platelet-derived growth factor (PDGF) was obtained from Amgen Biologicals. All other chemicals were of the highest purity available.Cell Culture. Swiss 3T3 and 3T6 mouse fibroblasts, human A431 epidermoid carcinoma cells (14), and DDT1-MF2 cells (35), derived from a vas deferens tumor of the hamster, were maintained in Dulbecco's modified Eagle's medium (DMEM; GIBCO) containing 10 mM Hepes, 44 mM NaHCO3, penicillin (110 units/ml), streptomycin (100 ,ug/ml), and 0.5% (vol/vol) (3T6), 5% (A431, DDT1-MF2), or 10% (3T3) FBS.Cells were grown at 37°C in a humidified atmosphere containing 5% CO2 and 95% air. Two days after seeding, the 3T3 cells were given fresh medium supplemented as before and then allowed to become confluent and quiescent for 5 days. The A431, DDT1-MF2, and 3T6 cultures were used 4, 5, or 7904The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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ATP‐induced calcium mobilization and inositol 1,4,5‐trisphosphate formation in H‐35 hepatoma cells

Cited by 45 publications

References 13 publications

Activation of early events of the mitogenic response by a P2Y purinoceptor with covalently bound 3'-O-(4-benzoyl)-benzoyladenosine 5'-triphosphate.

Activation of early events of the mitogenic response by a P2Y purinoceptor with covalently bound 3'-O-(4-benzoyl)-benzoyladenosine 5'-triphosphate.

Extracellular ATP induces Ca²⁺ transients in cardiac myocytes which are potentiated by norepinephrine

Extracellular ATP is a mitogen for 3T3, 3T6, and A431 cells and acts synergistically with other growth factors.

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ATP‐induced calcium mobilization and inositol 1,4,5‐trisphosphate formation in H‐35 hepatoma cells

Cited by 45 publications

References 13 publications

Activation of early events of the mitogenic response by a P2Y purinoceptor with covalently bound 3'-O-(4-benzoyl)-benzoyladenosine 5'-triphosphate.

Activation of early events of the mitogenic response by a P2Y purinoceptor with covalently bound 3'-O-(4-benzoyl)-benzoyladenosine 5'-triphosphate.

Extracellular ATP induces Ca2+ transients in cardiac myocytes which are potentiated by norepinephrine

Extracellular ATP is a mitogen for 3T3, 3T6, and A431 cells and acts synergistically with other growth factors.

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Extracellular ATP induces Ca²⁺ transients in cardiac myocytes which are potentiated by norepinephrine