ATP-enhanced peroxidase-like activity of gold nanoparticles

Shah, Juhi; Purohit, R.K.; Singh, Ragini; Karakoti, Ajay; Singh, Sanjay

doi:10.1016/j.jcis.2015.06.015

Cited by 111 publications

(53 citation statements)

References 50 publications

(13 reference statements)

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“…3(b), black line). To the best of our knowledge, the peak absorbance at 650 nm has been frequently used as a diagnostic peak in previous studies which exploited the peroxidase-mimicking property of AuNPs [30][31][32][33]. However, chemical and physical interferences may have the potential to induce AuNP aggregation, particularly in a medium with high electrolyte content, resulting in a red-shift of the plasmon peak around 650-700 nm.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, studies have focused on the suppression and enhancement of peroxidase-mimicking activity of AuNPs for the detection of H2O2, glucose, DNA and heavy metals. Functional molecules such as aptamers, DNA, antibodies, as well as cystamine and heavy metals have been utilised to tune the enzyme-mimicking activity of AuNPs in the design of biological sensors [30][31][32][33].…”

Section: Introductionmentioning

confidence: 99%

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Unusual switchable peroxidase-mimicking nanozyme for the determination of proteolytic biomarker

et al. 2018

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Detection of enzyme biomarkers originating from either bio-fluids or contaminating microorganisms is of utmost importance in clinical diagnostics and food safety. Herein, we present a simple, low-cost and easy-to-use sensing approach based on the switchable peroxidase-mimicking activity of plasmonic gold nanoparticles (AuNPs) that can catalyse for the oxidation of 3,3',5'5-tetramethylbenzidine (TMB) for the determination of protease enzyme. The AuNP surface is modified with casein, showing dual functionalities. The first function of the coating molecule is to suppress the intrinsic peroxidase-mimicking activity of AuNPs by up to 77.1%, due to surface shielding effects. Secondly, casein also functions as recognition sites for the enzyme biomarker. In the presence of protease, the enzyme binds to and catalyses the degradation of the coating layer on the AuNP surface, resulting in the recovery of peroxidase-mimicking activity. This is shown visually in the development of a blue colored product (oxidised TMB) or spectroscopically as an increase in absorbance at 370 and 650 nm. This mechanism allows for the detection of protease at 44 ng•mL −1 in 90 min. The nanosensor circumvents issues associated with current methods of detection in terms of ease of use, compatibility with point-of-care testing, low-cost production and short analysis time. The sensing approach has also been applied for the detection of protease spiked in ultra-heat treated (UHT) milk and synthetic human urine samples at a limit of detection of 490 and 176 ng•mL −1 , respectively, showing great potential in clinical diagnostics, food safety and quality control.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Unusual switchable peroxidase-mimicking nanozyme for the determination of proteolytic biomarker

et al. 2018

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“…After entry of the nanomedicine into the nervous system it attracts and binds to the microbes. Thereafter, it carries out the following steps: (1) it destroys the peptidoglycan (membrane) layer of the microbe, leading to the control of microbial growth; (2) it releases toxic metal ions into the cytosolic region of the microbe and can cause microbial death; (3) it alters the cellular ionic environment by activating the proton efflux pumps, leading to a change in pH; (4) it enhances the generation of free radicals, especially reactive oxygen species (ROS), leading to raised oxidative stress; (5) it damages the genetic material of the microbial organism, thus stopping the regulation of microbial growth and replication; and (6) it reduces ATP production, thus increasing energy demand and controlling microbial growth and proliferation (Rizzello et al, 2013;Upadya et al, 2011;Watkins et al, 2015;Shah et al, 2015). The molecular mechanism of nanomedicine for the elimination of microbes from the nervous system is illustrated in Fig.…”

Section: Molecular Mechanism Of Nanomedicine For Neuroinfectious Diseasementioning

confidence: 99%

Antimicrobial Nanostructures for Neurodegenerative Infections

Muthuraman

Kaur

2017

Nanostructures for Antimicrobial Therapy

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“…Artificial enzyme mimics have been attracting increasing attention over the past few years (Wiester, Ulmann, & Mirkin, 2011). Recently, gold nanoparticles (AuNPs) were reported to exhibit peroxidase enzyme-like properties, and they have been applied in bionics, biosensings and biomedicines (Deng et al, 2014;Lin, Ren, & Qu, 2014;Shah, Purohit, Singh, Karakoti, & Singh, 2015). The appearance and recent progress of nanotechnology provides new opportunities for the development of nanoparticles, which have stable and high catalytic activity (Yamada et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Development of novel biomimetic enzyme-linked immunosorbent assay method based on Au@SiO₂ nanozyme labelling for the detection of sulfadiazine

Liu

Jiang

et al. 2020

Food and Agricultural Immunology

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In the current study, a rapid and novel biomimetic nano-enzymelinked immunosorbent assay has been established for the detection of sulfadiazine (SDZ) using Au@SiO 2 nanoparticles labelling as a marker. In this system, gold nanoparticles exhibited intrinsic peroxidase and activity with excellent water-solubility, and the SiO 2 nanoparticles as ideal carries possessed a large surface area and ease of surface functionalization. The molecularly imprinted polymer films were selected as bionic antibody because of its advantages of selective recognition and reusability. Combine the above advantages, the method exhibited good stability, recognition capability and sensitivity. Under the optimal condition, the limit of detection of the immunosorbent assay was 0.2 mg/L. Beef sample spiked with SDZ was analysed, and the recoveries were in the 78.00% to 90.96% range. The assay could be used to detect SDZ residues in pork and chicken samples, and the results were not significantly different from those obtained by highperformance liquid chromatography.

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ATP-enhanced peroxidase-like activity of gold nanoparticles

Cited by 111 publications

References 50 publications

Unusual switchable peroxidase-mimicking nanozyme for the determination of proteolytic biomarker

Unusual switchable peroxidase-mimicking nanozyme for the determination of proteolytic biomarker

Antimicrobial Nanostructures for Neurodegenerative Infections

Development of novel biomimetic enzyme-linked immunosorbent assay method based on Au@SiO₂ nanozyme labelling for the detection of sulfadiazine

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ATP-enhanced peroxidase-like activity of gold nanoparticles

Cited by 111 publications

References 50 publications

Unusual switchable peroxidase-mimicking nanozyme for the determination of proteolytic biomarker

Unusual switchable peroxidase-mimicking nanozyme for the determination of proteolytic biomarker

Antimicrobial Nanostructures for Neurodegenerative Infections

Development of novel biomimetic enzyme-linked immunosorbent assay method based on Au@SiO2 nanozyme labelling for the detection of sulfadiazine

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Product

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Development of novel biomimetic enzyme-linked immunosorbent assay method based on Au@SiO₂ nanozyme labelling for the detection of sulfadiazine