ATP-Citrate Lyase Localized with the Peroxidase-Antiperoxidase Technique in RAT Heart and Liver

Abstract

Enzyme kinetic data has indicated the probability of “soluble” enzymes existing in situ as macromolecular complexes. These enzymes often function in sequential reactions, e. g., mitochondrial Krebs cycle. Therefore, it is important to define morphologically their functional in situ localization. One such enzyme of interest is ATP-citrate lyase (citrate cleavage enzyme). Biochemically, it has been characterized as a cytoplasmic enzyme. However, there are teleological as well as other indications that it may be concentrated in the vicinity of mitochondria.