ATP citrate lyase 1 (acl1) gene-based loop-mediated amplification assay for the detection of the Fusarium tricinctum species complex in pure cultures and in cereal samples

Niessen, Ludwig; Gräfenhan, Tom; Vogel, Rudi F.

doi:10.1016/j.ijfoodmicro.2012.06.021

Cited by 44 publications

(33 citation statements)

References 78 publications

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“…The method uses four or six different primers specifically designed to recognize six distinct regions in the target sequence (Eiken Chemical Co., Ltd.) and uses Bst DNA polymerase to interact with the template DNA during DNA replication. Bst DNA polymerase has a very high activity; thus, vast amounts of high-molecularweight DNA can be produced within a short time (28), with the reaction proceeding at a constant temperature. LAMP was first introduced in 2000, and it has been successfully used for the detection of many pathogens (28)(29)(30)(31).…”

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confidence: 99%

Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species

et al. 2014

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This study aimed to develop a loop-mediated isothermal amplification (LAMP) method for the rapid detection of Arcobacter species. Specific primers targeting the 23S ribosomal RNA gene were used to detect Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii. The specificity of the LAMP primer set was assessed using DNA samples from a panel of Arcobacter and Campylobacter species, and the sensitivity was determined using serial dilutions of Arcobacter species cultures. LAMP showed a 10-to 1,000-fold-higher sensitivity than multiplex PCR, with a detection limit of 2 to 20 CFU per reaction in vitro. Whereas multiplex PCR showed cross-reactivity with Campylobacter species, the LAMP method developed in this study was more sensitive and reliable than conventional PCR or multiplex PCR for the detection of Arcobacter species.

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confidence: 99%

Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species

et al. 2014

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“…As a consequence, DNA purification stage is time-consuming, troublesome, labor and capital-intensive and it would desirable to eliminate DNA purification. Previous researches have suggested that LAMP assay have shown a significant tolerance to inhibitor substances derived from blood, clinical samples, culture media [46,50], natural materials [18,51,52], and soil [53]. In the current study, the direct-LAMP was successfully developed which is free of DNA purification (samples prepared with minor treatment that merely took 10-20 min) and decreased cost, time, labor and also environmentally dangerous reagents.…”

Section: Discussionmentioning

confidence: 95%

Development and Application of Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Fusarium Oxysporum f. Sp. lycopersici

Almasi¹

2015

J Plant Pathol Microbiol

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A reliable and rapid pathogen detection protocol that utilizes colorimetric loop-mediated isothermal amplification (LAMP) was developed for detection of Fusarium oxysporum f. sp. lycopersici In this regard, all four LAMP primers (i.e. F3, B3, FIP and BIP) together with PCR primers (F and B) were designed based on conserved sequence of 28s ribosomal RNA gene which conserve between Fusarium oxysporum f. sp. lycopersici isolates (GenBank accession number: HM057281.1). Even though PCR and LAMP assays could successfully detect positive infected samples, considering the time, safety, cost and simplicity, the latter was overall superior. Furthermore, the results demonstrated that the LAMP assay was more sensitive and faster compared to PCR. Interestingly, LAMP reaction could successfully detect Fusarium oxysporum f. sp. lycopersici without DNA purification (direct-LAMP). Meanwhile, among six visual dyes used to detect LAMP products, hydroxynaphthol blue, GeneFinder TM and SYBR Green I could produce long stable color change and brightness in a close tube-based approach to prevent cross-contamination risk. Altogether, as LAMP is sensitive, cost effective, fairly user friendly and also can generate more accurate results than previous diagnostic procedures such as serological methods, PCR and other molecular methods, we accordingly propose this colorimetric assay as a highly reliable alternative fungi recognition system regarding Fusarium oxysporum f. sp. lycopersici recognition and probably other fungi-based diseases.

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“…This methods has been used to detect plant pathogenic fungi like Phytophthora ramorum (Tomlinson et al, 2007), Phytophthora sojae (Dai et al, 2012), A. flavus andA. parasiticus (Ahmed et al, 2010), toxigenic Fusarium (Denschlag et al, 2012;Niessen et al, 2012). The current study describes the LAMP for the detection of F. garmanirum in infested feed sample.…”

Section: Introductionmentioning

confidence: 98%

“…LAMP was also developed for rapid detection of pathogenic or allergenic fungal in the environment (Sun et al, 2010). Recently, LAMP-PCR using internal labeled probes have been developed for the major FHB pathogens in different plant materials (Niessen and Vogel, 2010;Abd-Elsalam et al, 2011;Niessen et al, 2012). LAMP depends on using a set of *Corresponding author.…”

Section: Introductionmentioning

confidence: 99%

One-hour loop-mediated isothermal amplification assay for the detection of quarantinable toxigenic Fusarium garmanirum

Almoammar¹,

Bahkali²,

Abd‐Elsalam³

2013

Afr. J. Microbiol. Res.

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Fusarium head blight (FHB) caused by severalFusarium species is one of the most serious diseases affecting wheat throughout the world. The in vitro production of the toxins deoxynivalenol, zearalenone fumonisin, T-2, and HT-2 was quantitvely evaluated in 8 different isolates of Fusarium species collected from feed samples. It was possible to detect zearalenone and the other mycotoxins in 100% and 50% of the isolates, respectively. In the present study, loop-mediated isothermal amplification method (LAMP) was designed for diagnosing Fusarium garmanirum infections and testing against feed samples, infested samples and pure cultures. The LAMP amplicon was directly visualized in the reaction tubes by the naked eye following the addition of calcein fluorescence. The LAMP products appeared as DNA marker pattern, with many bands of different sizes from 145 base pairs up to the loading well. Loop-LAMP procedure was used to detect genomic DNA of F. graminearum in fungal pure culture and in contaminated feed samples. In the future, this assay will support plant quarantine programs in Saudi Arabia and Gulf Cooperation Council states, to prevent the introduction of foreign FHB species.

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ATP citrate lyase 1 (acl1) gene-based loop-mediated amplification assay for the detection of the Fusarium tricinctum species complex in pure cultures and in cereal samples

Cited by 44 publications

References 78 publications

Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species

Comparison of Conventional PCR, Multiplex PCR, and Loop-Mediated Isothermal Amplification Assays for Rapid Detection of Arcobacter Species

Development and Application of Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Fusarium Oxysporum f. Sp. lycopersici

One-hour loop-mediated isothermal amplification assay for the detection of quarantinable toxigenic Fusarium garmanirum

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