ATP-Cell Viability Assay Methodology in Both Fresh Gynecologic Tumors and Cell Lines

Köchli, Ossi R.; Perras, James P.; Sevin, Bernd‐Uwe

doi:10.1159/000423479

Cited by 8 publications

(6 citation statements)

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“…Similar heterogeneity of fresh tumors in chemosensitivity testing with ATP-CVA has been shown already (28). Strikingly, the fresh tumor samples revealed a considerable variation of data.…”

Section: Discussionsupporting

confidence: 75%

“…The ATP-CVA is based on the principle of bioluminescence of cellular ATP, which is a substrate for the chemical reaction of luciferin/luciferase (27,28,(32)(33)(34)(35). Cellular ATP is produced by living cells only.…”

Section: Atp-cell-viability-assay (Bioluminescence Method)mentioning

confidence: 99%

“…We used tetra-(m-hydroxyphenyl)chlorin (m-THPC, Foscan ® ), a second generation photosensitizer (14,25) due to its propensity for good photodetection on the basis of autofluorescence (18) and its low toxicity in the dark (14,22,23,26). This method was originally described for chemosensitivity testing of fresh tumor cells in vitro (27,28). This method was originally described for chemosensitivity testing of fresh tumor cells in vitro (27,28).…”

Section: Introductionmentioning

confidence: 99%

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Photodynamic Effects In Vitro in Fresh GynecologicTumors Analyzed with a Bioluminescence Method

Schlosser¹,

Koechli²,

Cattaneo³

et al. 1999

CCLM

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Photodynamic therapy (PDT) is a promising alternative method for clinical cancer treatment. In the present study, cells from four breast carcinomas, seven ovarian carcinomas of various stages of differentiation, and ascites from a diffuse metastatic tumor were treated by PDT in vitro. Tetra(m-hydroxyphenyl)-chlorin (m-THPC) was used as the photosensitizer. Surviving cell rate was evaluated by the ATP-Cell-Viability-Assay (ATP-CVA), which measures light production as an interaction of intracellular ATP with the luciferin-luciferase complex. The most effective PDT of the tumor cells was achieved at an m-THPC concentration of 0.2 microgram/ml following incubation of the cells with photosensitizer for 24 hours. PDT toxicity resulted in a cell survival rate of 1% to 42% compared to untreated control cells (survival rate of control = 100%). The inhibitor concentration IC50 of m-THPC was determined both in the dark (dark toxicity) and in combination with laser irradiation. IC50 was defined as the concentration of photosensitizer which caused 50% of cell death. The IC50 values were heterogeneous in all tumor specimens examined. IC50 values for dark toxicity were on average 0.14 microgram m-THPC/ml for primary ovarian carcinoma, 2.16 micrograms m-THPC ml for refractory ovarian carcinoma and 0.3 microgram m-THPC/ml for breast carcinoma. After PDT, average IC50 value for refractory ovarian carcinoma was 0.04 microgram m-THPC/ml, for primary ovarian carcinoma 0.05 microgram m-THPC/ml and for breast carcinoma 0.03 microgram m-THPC/ml. These data might indicate that clinical PDT of gynecological carcinoma requires individual treatment conditions to achieve optimal results.

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“…Similar heterogeneity of fresh tumors in chemosensitivity testing with ATP-CVA has been shown already (28). Strikingly, the fresh tumor samples revealed a considerable variation of data.…”

Section: Discussionsupporting

confidence: 75%

Section: Atp-cell-viability-assay (Bioluminescence Method)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Photodynamic Effects In Vitro in Fresh GynecologicTumors Analyzed with a Bioluminescence Method

Schlosser¹,

Koechli²,

Cattaneo³

et al. 1999

CCLM

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“…Chemosensitivity was evaluated according to the study protocol at 6 concentrations of the drugs (0.125 ´-4.0 ´PPC; PPC = peak plasma concentration) using an in-vitro ATP cell viability assay [11,12,23]. In brief, the term PCC refers to the maximum plasma concentration achievable in patients.…”

Section: Chemosensitivity Testing Of Fresh Samples Of Human Ovarianmentioning

confidence: 99%

Dexamethasone-Induced Enhancement of Resistance to Ionizing Radiation and Chemotherapeutic Agents in Human Tumor Cells

Mariotta

Perewusnnyk

Koechli

et al. 1999

Strahlentherapie und Onkologie

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In agreement with previous reports, we found that dexamethasone may induce radioresistance in human carcinoma cells. Including the published data from the literature, dexamethasone induced enhancement in radioresistance in 4 of 12 carcinoma cell lines (33%), but not in 3 glioblastoma cell lines, nor in 3 fibroblast strains. Dexamethasone also induced enhanced resistance to carboplatin with a similar probability in fresh samples of ovarian cancer evaluated prospectively (in 4 of 13 samples; 31%). We worry that induction of resistance by corticosteroids given to patients undergoing either radiotherapy or chemotherapy with agents causing DNA damage might be associated with a reduced clinical responsiveness in a significant fraction of patients with a carcinoma.

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“…Dabei spiegelt der ATP-Gehalt der Zellen das Wachstumspotenzial der Zelle wider. Beim Zelltod fällt der ATP-Gehalt auf Null [1,4,11]. Die ursprünglich bei 30 Tumorproben durchgeführten Messungen reduzierten sich bei der Auswertung dadurch, dass von 2 Patientinnen die Eingaben fehlerhaft waren und dass es sich bei 6 Proben histologisch um Adenokarzinome der Cervix uteri handelte.…”

Section: Abstract !unclassified

Chemosensitivitätstestungen beim Kollumkarzinom

Husstedt¹,

Jenz²,

Bauer³

et al. 2007

Geburtsh Frauenheilk

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ATP-Cell Viability Assay Methodology in Both Fresh Gynecologic Tumors and Cell Lines

Cited by 8 publications

References 0 publications

Photodynamic Effects In Vitro in Fresh GynecologicTumors Analyzed with a Bioluminescence Method

Photodynamic Effects In Vitro in Fresh GynecologicTumors Analyzed with a Bioluminescence Method

Dexamethasone-Induced Enhancement of Resistance to Ionizing Radiation and Chemotherapeutic Agents in Human Tumor Cells

Chemosensitivitätstestungen beim Kollumkarzinom

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