ATP Bioluminescence Procedure for Viability Testing of Potential Beer Spoilage Microorganisms

Dowhanick, Terrance M.; Sobczak, Jadwiga

doi:10.1094/asbcj-52-0019

Cited by 14 publications

(9 citation statements)

References 3 publications

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“…Assays of ATP content have been widely used to characterise biomass viability [13] and to detect potential spoilage microorganisms in the beer and food industry [8,12], assuming that it is possible to use the concentration of ATP to measure the viable cells of a certain species. In the work mentioned above, ATP content was determined to give information about the general energetic state of the culture when submitted to the toxicants.…”

Section: Biochemical Toxicological Assaysmentioning

confidence: 99%

Trends in the use of protozoa in the assessment of wastewater treatment

Nicolau

Dias

Мота

et al. 2001

Research in Microbiology

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Increasing environmental pollution and the continuous development of new chemicals and drugs has led to ever growing concern about the potential effects of these compounds directly or indirectly on human health. As concerns water pollution, protozoa seem to be an excellent tool to assess both toxicity and pollution: they are regarded as biological indicators of pollution when their presence or absence can be related to particular environmental conditions, and they are considered test organisms when a species or population is used to evaluate the toxicity of relevant toxic compounds. Thus, an integrated approach is being developed to assess how toxic compounds affect the different biological levels of organisation--from the community level to the species level--of ciliated protozoa. The present paper reports and discusses the current state of the art of this approach.

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Section: Biochemical Toxicological Assaysmentioning

confidence: 99%

Trends in the use of protozoa in the assessment of wastewater treatment

Nicolau

Dias

Мота

et al. 2001

Research in Microbiology

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“…According to Connolly and colleagues (1993) and Dowhanick and Sobczak (1994), one of the major problems with pharmaceutical formulation is possible interference of formulation constituents with detection of the light emission. Formulation constituents can absorb or disperse emitted light, which severely limits the detection sensitivity and consequently leads to the occurence of false-positive results.…”

Section: Resultsmentioning

confidence: 99%

Preservative Efficacy Screening of Pharmaceutical Formulations Using ATP Bioluminescence

Kramer¹,

Šuklje-Debeljak

Kmetec

2008

Drug Development and Industrial Pharmacy

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The preservative challenge test is a method used to determine the efficacy of a preservation system in a pharmaceutical or cosmetic formulation. However, such testing is a labor-intensive, repetitive task often requiring days before results can be generated. Several alternatives to traditional colony-count techniques have been developed. A study using pure suspensions of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Bacillus subtilis, Candida albicans, and Aspergillus niger showed that the accuracy, repeatability, and linearity of the Pallchek™ luminometer ATP bioluminescence (ATP-B) system was equivalent to the traditional colony-count method. In any case, the method proved sensitive enough to follow the effect of preservatives on a number of test microorganisms, indicating the applicability of the ATP-B method for preservative screening studies in various pharmaceutical formulations.

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“…There is an increasing demand for new technologies that provide rapid quanti®cation of micro-organisms in suspension. Many methods have been evaluated, including epi¯uor escence microscopy,¯ow cytometry and bioluminescence (Dowhanick and Socczak 1994;Kepner and Pratt 1994;Davey et al 1999). The measurement of biological systems using such methods has proved dif®cult, with constraints such as selectivity, sensitivity, reproducibility and cost.…”

Section: Introductionmentioning

confidence: 99%

A rapid, non-destructive method for the determination of Staphylococcus epidermidis adhesion to surfaces using quartz crystal resonant sensor technology

Pavey

Barnes

Hanlon

et al. 2001

Lett Appl Microbiol

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Aims: To investigate the use of quartz crystal resonant sensor (QCRS) technology to determine the adhesion of Staphylococcus epidermidis to ®bronectin-coated surfaces. Methods and Results: QCRS sensors (14 MHz) with 4 mm gold electrodes were coated with ®bronectin and exposed for 15 min to suspensions of Staph. epidermidis ranging in concentration from 1´10 2 to 1´10 6 cfu ml )1 . Changes in resonant frequency were recorded and showed a linear relationship with the logarithm of cell concentration over the range tested. Conclusions: QCRS technology was shown to be a rapid, sensitive and non-destructive method for measuring the adhesion of bacteria to surfaces. Signi®cance and Impact of the Study: This report demonstrates that QCRS technology has the potential to be used for a range of applications requiring measurement of bacteria on surfaces. In particular, it may be used for the real-time monitoring of bacterial bio®lm formation.

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ATP Bioluminescence Procedure for Viability Testing of Potential Beer Spoilage Microorganisms

Cited by 14 publications

References 3 publications

Trends in the use of protozoa in the assessment of wastewater treatment

Trends in the use of protozoa in the assessment of wastewater treatment

Preservative Efficacy Screening of Pharmaceutical Formulations Using ATP Bioluminescence

A rapid, non-destructive method for the determination of Staphylococcus epidermidis adhesion to surfaces using quartz crystal resonant sensor technology

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