ATP-binding cassette transporters expression profiling revealed its role in the development and regulating stress response in Solanum tuberosum

Zaynab, Madiha; Wang, Zongkang; Hussain, Athar; Bahadar, Khalida; Sajid, Mateen; Sharif, Yasir; Azam, Muhammad; Sughra, Kalsoom; Raza, Muhammad Ammar; Khan, Khalid Ali; Li, Shuangfei

doi:10.1007/s11033-021-06697-z

Cited by 3 publications

(6 citation statements)

References 45 publications

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“…This crucial gene family carried out vital defense mechanisms and is an essential part of plant defense processes. The expression of the essential genes increases exponentially when plants encounter stress like salinity ( Wang et al, 2013 ; Zaynab et al, 2021d ), water shortage ( Komatsu et al, 2010 ), heavy metals ( Berna and Bernier, 1999 ), and heat stress ( Qasim et al, 2018b ). Various functions of GLPs have also been determined in different crops such as Hordeum vulgare ( Zimmermann et al, 2006 ), A. thaliana ( Rietz et al, 2012 ), Oryza sativa ( Li et al, 2016 ), and Glycine max ( Wang et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

“…In this study, we used two methods to identify GLP genes from the genome of the Solanum tuberosum. The first method was the Hidden Markov Model (HMM), and the second one was the BLASTP search ( Raza et al, 2021 ; Zaynab et al, 2021d ). Potato genome sequences were retrieved from JGI Phytozome 12.0 1 database ( Goodstein et al, 2012 ).…”

Section: Methodsmentioning

confidence: 99%

“…In order to investigate the expression of StGLP genes in various tissues and under various stress conditions, the transcriptome data were obtained from the available public database BioProject ID: Project: PRJEB2430 . For the expression analysis of StGLP genes in different tissues and under different stresses, fragments per kilobase million (FPKM) were observed ( Zaynab et al, 2021d ). The obtained data were arranged according to their expression in different tissues.…”

Section: Methodsmentioning

confidence: 99%

“…About 368,168,914 tons of potatoes were harvested in 2018 on 17,578,672 ha of land, and over 1 billion people consumed them ( Rashid et al, 2021 ). Like other plant species, potato yield is also in jeopardy due to many biotic and abiotic environmental fluctuations ( Zaynab et al, 2021d ). To date, the expression analysis of GLP genes against abiotic stress has not been reported in potatoes.…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification and Expression Profiling of Germin-Like Proteins Reveal Their Role in Regulating Abiotic Stress Response in Potato

et al. 2022

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Germin and germin-like proteins (GLPs) perform a significant role in plants against biotic and abiotic stress. To understand the role of GLPs in potato, a comprehensive genome-wide analysis was performed in the potato genome. This study identified a total of 70 StGLPs genes in the potato genome, distributed among 11 chromosomes. Phylogenetic analysis exhibited that StGLPs were categorized into six groups with high bootstrap values. StGLPs gene structure and motifs analysis showed a relatively well-maintained intron–exon and motif formation within the cognate group. Additionally, several cis-elements in the promoter regions of GLPs were hormones, and stress-responsive and different families of miRNAs target StGLPs. Gene duplication under selection pressure also exhibited positive and purifying selections in StGLPs. In our results, the StGLP5 gene showed the highest expression in response to salt stress among all expressed StGLPs. Totally 19 StGLPs genes were expressed in response to heat stress. Moreover, three genes, StGLP30, StGLP17, and StGLP14, exhibited a relatively higher expression level in the potato after heat treatment. In total, 22 genes expressed in response to abscisic acid (ABA) treatment indicated that ABA performed an essential role in the plant defense or tolerance mechanism to environmental stress. RNA-Seq data validated by RT-qPCR also confirm that the StGLP5 gene showed maximum expression among selected genes under salt stress. Concisely, our results provide a platform for further functional exploration of the StGLPs against salt and heat stress conditions.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification and Expression Profiling of Germin-Like Proteins Reveal Their Role in Regulating Abiotic Stress Response in Potato

et al. 2022

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“…These results are supported by previous studies on GLP genes ( Lee and Lee, 2003 ; Komatsu et al., 2010 ). Hormones regulate a genes’ biochemical and physiological reactions through different signal transduction pathways ( Fatima et al., 2021 ; Zaynab et al., 2021 ; Raza et al., 2022b ). Drought is a major limiting factor for crop yields.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification of germin-like proteins in peanut (Arachis hypogea L.) and expression analysis under different abiotic stresses

et al. 2023

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Peanut is an important food and feed crop, providing oil and protein nutrients. Germins and germin-like proteins (GLPs) are ubiquitously present in plants playing numerous roles in defense, growth and development, and different signaling pathways. However, the GLP members have not been comprehensively studied in peanut at the genome-wide scale. We carried out a genome-wide identification of the GLP genes in peanut genome. GLP members were identified comprehensively, and gene structure, genomic positions, motifs/domains distribution patterns, and phylogenetic history were studied in detail. Promoter Cis-elements, gene duplication, collinearity, miRNAs, protein-protein interactions, and expression were determined. A total of 84 GLPs (AhGLPs ) were found in the genome of cultivated peanut. These GLP genes were clustered into six groups. Segmental duplication events played a key role in the evolution of AhGLPs, and purifying selection pressure was underlying the duplication process. Most AhGLPs possessed a well-maintained gene structure and motif organization within the same group. The promoter regions of AhGLPs contained several key cis-elements responsive to ‘phytohormones’, ‘growth and development’, defense, and ‘light induction’. Seven microRNAs (miRNAs) from six families were found targeting 25 AhGLPs. Gene Ontology (GO) enrichment analysis showed that AhGLPs are highly enriched in nutrient reservoir activity, aleurone grain, external encapsulating structure, multicellular organismal reproductive process, and response to acid chemicals, indicating their important biological roles. AhGLP14, AhGLP38, AhGLP54, and AhGLP76 were expressed in most tissues, while AhGLP26, AhGLP29, and AhGLP62 showed abundant expression in the pericarp. AhGLP7, AhGLP20, and AhGLP21, etc., showed specifically high expression in embryo, while AhGLP12, AhGLP18, AhGLP40, AhGLP78, and AhGLP82 were highly expressed under different hormones, water, and temperature stress. The qRT-PCR results were in accordance with the transcriptome expression data. In short, these findings provided a foundation for future functional investigations on the AhGLPs for peanut breeding programs.

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GmABCG5, an ATP-binding cassette G transporter gene, is involved in the iron deficiency response in soybean

Wang,

Zhang,

Yan

et al. 2024

Front. Plant Sci.

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Iron deficiency is a major nutritional problem causing iron deficiency chlorosis (IDC) and yield reduction in soybean, one of the most important crops. The ATP-binding cassette G subfamily plays a crucial role in substance transportation in plants. In this study, we cloned the GmABCG5 gene from soybean and verified its role in Fe homeostasis. Analysis showed that GmABCG5 belongs to the ABCG subfamily and is subcellularly localized at the cell membrane. From high to low, GmABCG5 expression was found in the stem, root, and leaf of young soybean seedlings, and the order of expression was flower, pod, seed stem, root, and leaf in mature soybean plants. The GUS assay and qRT-PCR results showed that the GmABCG5 expression was significantly induced by iron deficiency in the leaf. We obtained the GmABCG5 overexpressed and inhibitory expressed soybean hairy root complexes. Overexpression of GmABCG5 promoted, and inhibition of GmABCG5 retarded the growth of soybean hairy roots, independent of nutrient iron conditions, confirming the growth-promotion function of GmABCG5. Iron deficiency has a negative effect on the growth of soybean complexes, which was more obvious in the GmABCG5 inhibition complexes. The chlorophyll content was increased in the GmABCG5 overexpression complexes and decreased in the GmABCG5 inhibition complexes. Iron deficiency treatment widened the gap in the chlorophyll contents. FCR activity was induced by iron deficiency and showed an extraordinary increase in the GmABCG5 overexpression complexes, accompanied by the greatest Fe accumulation. Antioxidant capacity was enhanced when GmABCG5 was overexpressed and reduced when GmABCG5 was inhibited under iron deficiency. These results showed that the response mechanism to iron deficiency is more actively mobilized in GmABCG5 overexpression seedlings. Our results indicated that GmABCG5 could improve the plant’s tolerance to iron deficiency, suggesting that GmABCG5 might have the function of Fe mobilization, redistribution, and/or secretion of Fe substances in plants. The findings provide new insights into the ABCG subfamily genes in the regulation of iron homeostasis in plants.

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ATP-binding cassette transporters expression profiling revealed its role in the development and regulating stress response in Solanum tuberosum

Cited by 3 publications

References 45 publications

Genome-Wide Identification and Expression Profiling of Germin-Like Proteins Reveal Their Role in Regulating Abiotic Stress Response in Potato

Genome-Wide Identification and Expression Profiling of Germin-Like Proteins Reveal Their Role in Regulating Abiotic Stress Response in Potato

Genome-wide identification of germin-like proteins in peanut (Arachis hypogea L.) and expression analysis under different abiotic stresses

GmABCG5, an ATP-binding cassette G transporter gene, is involved in the iron deficiency response in soybean

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