ATP-binding cassette proteins BCRP, MRP1 and P-gp expression and localization in the human umbilical cord

Riches, Zoe; Walia, Gurinder Kaur; Berman, Jacob M.; Wright, Tricia E.; Collier, Abby C.

doi:10.3109/00498254.2015.1091118

Cited by 7 publications

(3 citation statements)

References 46 publications

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“…Meanwhile, 231/DDP cell line had a lower intracellular DDP concentration compared with 231/wt cell line, indicating 231/DDPcell line had a higher excretive ability of DDP, which might explain its strong drug resistance. Moreover, expressions of three drug-resistant membrane proteins (MRP1, P-gP and BCRP) [ 19 , 20 ] were remarkably increased in 231/DDP cell line. Collectively, these might be the physiochemical and molecular basis of DDP-drug resistance mechanisms.…”

Section: Discussionmentioning

confidence: 99%

ETS1 is associated with cisplatin resistance through IKKα/NF-κB pathway in cell line MDA-MB-231

Zhang

et al. 2018

Cancer Cell Int

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BackgroundPlatinum-based drugs are used extensively in neoadjuvant chemotherapy for triple-negative breast cancer (TNBC), but their use can be limited by resistance. In this study, we established cisplatin (DDP) resistant TNBC cells to investigate the potential relationship among ETS1, IKKα/NF-κB and resistance.MethodsThe sensitivity was evaluated by MTT, apoptosis analysis. The intracellular DDP concentration difference was tested by inductively coupled plasma mass spectrometry (ICP-MS) method. Molecular pathological mechanism of DDP resistance was explored by microarray analysis and PPI network analysis. The ETS1, NF-κB signaling change were assessed by western blot and q-PCR in vitro and vivo. The existing binds between ETS1 and the core IKKα promoter were found by luciferase assay and chromatin immunoprecipitation technique (ChIP).ResultsMDA-MB-231/DDP (231/DDP) cell had a higher IC50 value of cisplatin, lower intracellular DDP concentration, and lower apoptosis ratio than MDA-MB-231 (231/wt) cell line treated with DDP. Increased ABC transporters were induced by the activation of NF-κB pathway in 231/DDP cells. ETS1, RPL6, RBBP8, BIRC2, PIK3A and RARS were six important genes for DDP-resistance based on PPI network and expression validation. Protein expression of ETS1 and IKKα were significantly up-regulated in 231/DDP cells. However, inhibition of ETS1 expression enhances chemo-sensitivity to DDP and reversed the activation of NF-κB pathway in 231/DDP cells and subcutaneous transplantation tumor in vivo. Moreover, there is existing binds between ETS1 and the core IKKα promoter though luciferase assay and ChIP.ConclusionThis study enables us to understand the functions of ETS1 in TNBC chemotherapy and suggests that ETS1 could be used as a novel marker of poor response to DDP and a potential therapeutic target for TNBC chemotherapy.

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Section: Discussionmentioning

confidence: 99%

ETS1 is associated with cisplatin resistance through IKKα/NF-κB pathway in cell line MDA-MB-231

Zhang

et al. 2018

Cancer Cell Int

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“…The ABC transporter genes are classified into seven subfamilies (A to G). Among these transporters, ABCB1 (P-glycoprotein/P-gp/MDR1), ABCC1 (multidrug resistance protein 1/MDR1), and ABCG2 (breast cancer resistance protein/BCRP) have been extensively reported in many studies [ 160 ]. The most common indicator of multidrug resistance is P-glycoprotein (P-gp), which is a product of the multidrug resistance gene (MDR1) and a 170 kDa transmembrane phosphoglycoprotein that functions as an ATP-dependent efflux pump [ 161 ].…”

Section: Stem Cells and Changes In Drug Efflux (Drug Transporters)mentioning

confidence: 99%

Impact of Non-Coding RNAs on Chemotherapeutic Resistance in Oral Cancer

et al. 2022

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Drug resistance in oral cancer is one of the major problems in oral cancer therapy because therapeutic failure directly results in tumor recurrence and eventually in metastasis. Accumulating evidence has demonstrated the involvement of non-coding RNAs (ncRNAs), such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), in processes related to the development of drug resistance. A number of studies have shown that ncRNAs modulate gene expression at the transcriptional or translational level and regulate biological processes, such as epithelial-to-mesenchymal transition, apoptosis, DNA repair and drug efflux, which are tightly associated with drug resistance acquisition in many types of cancer. Interestingly, these ncRNAs are commonly detected in extracellular vesicles (EVs) and are known to be delivered into surrounding cells. This intercellular communication via EVs is currently considered to be important for acquired drug resistance. Here, we review the recent advances in the study of drug resistance in oral cancer by mainly focusing on the function of ncRNAs, since an increasing number of studies have suggested that ncRNAs could be therapeutic targets as well as biomarkers for cancer diagnosis.

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“…ABCC1 expression has been shown in many species, and in human, ABCC1 mRNA is expressed in various tissues including placenta, liver, and kidney [ 12 , 13 ]. ABCC9 is a subunit of ATP-sensitive K + channel and is expressed in many tissues including myometrium, cardiac and skeletal muscle [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

Expression and regulation of prostaglandin transporters, ATP-binding cassette, subfamily C, member 1 and 9, and solute carrier organic anion transporter family, member 2A1 and 5A1 in the uterine endometrium during the estrous cycle and pregnancy in pigs

Jang

Choi

Yoo

et al. 2016

Asian-Australas J Anim Sci

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ObjectiveProstaglandins (PGs) function in various reproductive processes, including luteolysis, maternal pregnancy recognition, conceptus development, and parturition. Our earlier study has shown that PG transporters ATP-binding cassette, subfamily C, member 4 (ABCC4) and solute carrier organic anion transporter family, member 2A1 (SLCO2A1) are expressed in the uterine endometrium in pigs. Since several other PG transporters such as ABCC1, ABCC9, SLCO4C1, and SLCO5A1 are known to be present in the uterine endometrium, this study investigated the expression of these PG transporters in the porcine uterine endometrium and placenta.MethodsUterine endometrial tissues were obtained from gilts on day (D) 12 and D15 of the estrous cycle and days 12, 15, 30, 60, 90, and 114 of pregnancy.ResultsABCC1, ABCC9, SLCO4C1, and SLCO5A1 mRNAs were expressed in the uterine endometrium, and levels of expression changed during the estrous cycle and pregnancy. Expression of ABCC1 and ABCC9 mRNAs was localized mainly to luminal and glandular epithelial cells in the uterine endometrium, and chorionic epithelial cells during pregnancy. Conceptuses during early pregnancy and chorioallantoic tissues from mid to late pregnancy also expressed these PG transporters. Estradiol-17β increased the expression of ABCC1 and SLCO5A1, but not ABCC9 and SLCO4C1 mRNAs and increasing doses of interleukin-1β induced the expression of ABCC9, SLCO4C1, and SLCO5A1 mRNAs in endometrial explant tissues.ConclusionThese data showed that several PG transporters such as ABCC1, ABCC9, SLCO4C1, and SLCO5A1 were expressed at the maternal-conceptus interface, suggesting that these PG transporters may play an important role in the establishment and maintenance of pregnancy by regulating PG transport in the uterine endometrium and placenta in pigs.

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ATP-binding cassette proteins BCRP, MRP1 and P-gp expression and localization in the human umbilical cord

Cited by 7 publications

References 46 publications

ETS1 is associated with cisplatin resistance through IKKα/NF-κB pathway in cell line MDA-MB-231

ETS1 is associated with cisplatin resistance through IKKα/NF-κB pathway in cell line MDA-MB-231

Impact of Non-Coding RNAs on Chemotherapeutic Resistance in Oral Cancer

Expression and regulation of prostaglandin transporters, ATP-binding cassette, subfamily C, member 1 and 9, and solute carrier organic anion transporter family, member 2A1 and 5A1 in the uterine endometrium during the estrous cycle and pregnancy in pigs

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