Atorvastatin modifies the protein profile of circulating human monocytes after an acute coronary syndrome

Barderas, María G.; Tuñón, José; Dardé, Verónica M.; Cuesta, Fernando de la; Jiménez-Nacher, José Julio; Tarín, Nieves; López-Bescós, Lorenzo; Egido, Jesús; Vivanco, Fernando

doi:10.1002/pmic.200700583

Cited by 26 publications

(15 citation statements)

References 56 publications

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“…In contrast, there was overexpression of mature cathepsin D, with pro-atherogenic effects and enolase I, involved in macrophage transformation into foam cells. Other studies in the same context confirm that, in NSTE-ACS patients, atorvastatin 80 mg/day normalizes expression of HSP70, paraoxonase I, annexin I-which has antiinflammatory properties-and annexin II-involved in spontaneous fibrinolysis (Barderas et al, 2009). It has previously been shown that cathepsin D and HSP70 are biomarkers of atherosclerotic lesions .…”

Section: Proteomics: the Current Center Of Attentionsupporting

confidence: 60%

Translational Approaches In Cardiovascular Diseases by Omics

Davani¹

2018

Current Issues in Molecular Biology

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Cardiovascular diseases are among the leading causes of morbidity and mortality. Despite scientific and technical progress in risk prediction, diagnostics, prognostication and therapy of cardiovascular pathologies, new biomarkers and therapeutic targets remain the subject of intense research to reduce the burden of these diseases.High throughput analyses, termed "omics", are a promising avenue of research. These recently developed technical fields have revolutionized biological and medical research in a very short time. By their interdisciplinary nature, these new methods have already provided a wide vision of cell and tissue pathways and functions. Here, we review how these methods can help to discover new biomarkers and therapeutic targets in cardiovascular diseases.

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Section: Proteomics: the Current Center Of Attentionsupporting

confidence: 60%

Translational Approaches In Cardiovascular Diseases by Omics

Davani¹

2018

Current Issues in Molecular Biology

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“…With a similar approach we showed that atorvastatin 80 mg/day affected the expression of 20 proteins in NSTEACS patients as compared with moderate statin therapy (29). Among them, there was a normalization of the decreased expression of HSP70, paraoxonase I, annexin I-which has anti-inflammatory properties-and annexin II-involved in spontaneous fibrinolysis.…”

Section: The Study Of Blood By Proteomic Approachesmentioning

confidence: 54%

Proteomic Strategies in the Search of New Biomarkers in Atherothrombosis

Tuñón

Martı́n-Ventura

Blanco-Colio

et al. 2010

Journal of the American College of Cardiology

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Extensive research has focused on the identification of novel plasma biomarkers to improve our ability to predict cardiovascular events in atherothrombosis. However, classical techniques can only assess a limited number of proteins at a time. Given that plasma contains more than 900,000 proteins, this approach will be extremely time-consuming. Novel proteomic approaches make it possible to compare the expression of hundreds of proteins in several samples in a single experiment. The classical approach consists of separation of proteins on a 2-dimensional gel followed by protein identification with mass spectrometry, although new complementary gel-free techniques are emerging. We can thus compare protein expression in an atherosclerotic plaque with that in a normal artery or study plasma proteins in patients with atherothrombosis as compared with healthy subjects. For such approaches, it is not necessary to study the published data to select potential biomarkers. However, because the number of patients that can be studied with most of these techniques is limited, what is really important is the design of the studies, assessing carefully what kind of patients should be included to obtain valid conclusions. Clinicians should thus play a key role in this design along with the basic scientist. In this article, we review several proteomic strategies carried out by our group and others, and we make a call for collaboration between clinicians and experts in proteomics. This collaboration could greatly increase the likelihood of identifying new prognostic biomarker panels in atherothrombosis and other cardiovascular disorders.

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“…Proteomic studies performed to date in atherosclerosis involve plasma (13), circulating cells (particularly monocytes) (14,15), and atherome tissue analysis (7). Proteomic analysis of atherome tissue has been mostly carried out by studying the tissue as a whole.…”

Section: Discussionmentioning

confidence: 99%

A Proteomic Focus on the Alterations Occurring at the Human Atherosclerotic Coronary Intima

Cuesta

Alvarez-Llamas

Maroto

et al. 2011

Molecular & Cellular Proteomics

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Coronary atherosclerosis still represents the major cause of mortality in western societies. Initiation of atherosclerosis occurs within the intima, where major histological and molecular changes are produced during pathogenesis. So far, proteomic analysis of the atherome plaque has been mainly tackled by the analysis of the entire tissue, which may be a challenging approach because of the great complexity of this sample in terms of layers and cell type composition. Based on this, we aimed to study the intimal proteome from the human atherosclerotic coronary artery. For this purpose, we analyzed the intimal layer from human atherosclerotic coronaries, which were isolated by laser microdissection, and compared with those from preatherosclerotic coronary and radial arteries, using a two-dimensional Differential-In-Gel-Electrophoresis (DIGE) approach. Results have pointed out 13 proteins to be altered (seven up-regulated and six down-regulated), which are implicated in the migrative capacity of vascular smooth muscle cells, extracellular matrix composition, coagulation, apoptosis, heat shock response, and intraplaque hemorrhage deposition. Among these, three proteins (annexin 4, myosin regulatory light 2, smooth muscle isoform, and ferritin light chain) constitute novel atherosclerotic coronary intima proteins, because they were not previously identified at this human coronary layer. For this reason, these novel proteins were validated by immunohistochemistry, together with hemoglobin and vimentin, in an independent cohort of arteries. Molecular & Cellular

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Atorvastatin modifies the protein profile of circulating human monocytes after an acute coronary syndrome

Cited by 26 publications

References 56 publications

Translational Approaches In Cardiovascular Diseases by Omics

Translational Approaches In Cardiovascular Diseases by Omics

Proteomic Strategies in the Search of New Biomarkers in Atherothrombosis

A Proteomic Focus on the Alterations Occurring at the Human Atherosclerotic Coronary Intima

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