Atorvastatin mitigates testicular injuries induced by ionizing radiation in mice

Naeimi, Ramezan Ali; Amiri, Fereshteh Talebpour; Khalatbary, Ali Reza; Ghasemi, Arash; Zargari, Mehryar; Ghesemi, Maryam; Hosseinimehr, Seyed Jalal

doi:10.1016/j.reprotox.2017.06.052

Cited by 53 publications

(34 citation statements)

References 56 publications

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“…ATV through inhibition the generation of ROS have antioxidant properties (28). Protective effect of atorvastatin has been proven in the intestines (29), brain (30), kidney (15), heart (16), testis (17) and ovary (18) injury against induced ischemia/reperfusion, chemotherapy and irradiation. Chemoprotective and therapeutic effects of ATV has been seen against doxorubicin-induced hepato-renal toxicity (31).…”

Section: Discussionmentioning

confidence: 99%

“…After incubation (2 hr) with secondary antibody conjugated with horseradish peroxidase (Mouse and Rabbit Specific HRP/DAB, Abcam, Lat: GR2623314-4), sections were incubated (5 min) with diaminobenzidine tetrahydrochloride. Then, the samples were dehydrated and mounted (17). …”

Section: Methodsmentioning

confidence: 99%

“…But, atorvastatin at low dose does not have side effects on fertility and reproduction (14). Protective effect of atorvastatin was reported against nephrotoxicity (15), cardiotoxicity (16), testicular toxicity (17) and ovarian toxicity (18). So far, no study has not reported on the protective effect of ATV against ovarian injury induced by CP.…”

Section: Introductionmentioning

confidence: 99%

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Atorvastatin attenuates the ovarian damage induced by cyclophosphamide in rat: An experimental study

Hamzeh¹,

Hosseinimehr²,

Mohammadi³

et al. 2018

IJRM

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Background:Cyclophosphamide (CP), as an anticancer agent, causes ovarian toxicity and subsequent infertility in women. Atorvastatin (ATV) at a low dose has antioxidant and anti-inflammatory properties.Objective:The aim of this study was to investigate the protective effect of ATV against CP-induced ovarian injury in rat.Materials and Methods:In this experimental study, thirty-two female Wistar rats were randomly divided into four groups as I) control, II) ATV (10 mg/kg), III) CP (150 mg/kg), and IV) CP +ATV. The ATV treated groups were received ATV for 10 days via oral gavage. In the CP+ATV group, ATV was administrated on 5 days before and 5 days after CP injection. Histological structure, apoptosis (caspase-3), oxidative stress parameters as malondialdehyde, reactive oxygen species, protein carbonyl levels and cell viability were evaluated in ovary tissue by histological scores, immunohistochemistry, histochemical and biochemical assays. The levels of estrogen and progesterone hormones were measured on the 12th day of study.Results:ATV pretreatment significantly decreased the levels of oxidative stress biomarkers as malondialdehyde, reactive oxygen species and protein carbonyl levels and increased cell death in CP-treated rats as compared with the CP alone group. ATV significantly increased estrogen and progesterone levels in CP-treated rats. In addition, the histological examination showed ATV mitigated acute inflammation, degenerative cells in stroma and follicles, stromal edema, vacuolization, atresia of the follicles and congestion of blood vessels in the CP-treated animals. Furthermore, ATV significantly reduced immunoreactivity level of caspase-3 in CP-treated rats.Conclusion:Our results showed that the ATV with antioxidant and anti-apoptosis (caspase-3) activities protected ovarian against CP-induced toxicity.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Atorvastatin attenuates the ovarian damage induced by cyclophosphamide in rat: An experimental study

Hamzeh¹,

Hosseinimehr²,

Mohammadi³

et al. 2018

IJRM

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“…Then, the tissue sections were incubated with protein blocker for 10 minutes. After incubation at 4°C overnight with primary antibodies (anti-caspase 3 rabbit polyclonal antibody, 1:100 in PBS, v/v, Abcam, Lat: GR224831-2) at 4°C overnight, serial sections of tissues were intubated with secondary antibody conjugated with horseradish peroxidase (Mouse and Rabbit Specific HRP/DAB, Abcam, Lat: GR2623314-4) for 20 minutes, sections were incubated with diaminobenzidine tetrahydrochloride for 5 minutes [48]. Then the slides were rehydrated in alcohol series and dehydrated in xylene.…”

Section: Immunohistochemical Assaymentioning

confidence: 99%

Hydroalcoholic extract of Zataria multiflora mitigates cisplatin-induced oxidative stress, apoptosis and hepatotoxicity in mice

Karimi¹,

Amir²,

Khalatbary³

et al. 2018

jrp

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Cisplatin (CP), as an anticancer drug, causes hepatotoxicity. Zataria multiflora Boiss (ZM), as a herbal medicine, has antioxidant property and decrease oxidative stress-induced toxicity and side effects. This study investigated the effects of hydroalcoholic extract of ZM against cisplatin-induced hepatotoxicity. In this experimental study, thirty-two male mice were randomly divided into four groups (8/group). The control group; ZM group was received ZM (200 mg/kg) during 7 days by gavage; CP group was received CP (10 mg/kg) intraperitoneally in 5 th day of study; ZM + CP group. Serum biochemical [alanine aminotransferases (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP)], tissue biochemical [malondialdehyde (MDA), protein carbonyl (PC) and glutathione (GSH) content], histopathological and immunohistochemical assays were examined to determine hepatotoxicity. CP caused significant increase in ALT, AST and ALP, oxidative stress (increased of MDA, PC and decreased of GSH in liver tissues), histological changes (hepatocellular degeneration, necrosis, inflammatory cell infiltration, congestion, sinusoidal dilatation). ZM significantly mitigated the toxic effects of CP on the liver tissue and oxidative stress (p<0.01 and p<0.05, respectively). Furthermore, CP increased caspase-3 immunoreactivity (apoptosis) (21.85 ± 6.81) and ZM administration significantly reduced immunoreactivity of caspase-3 compared with CP-treated mice (10.66 ± 3.23). The findings of this study showed that the ZM as a potential antioxidant compound and with scavenging free radicals, hepatic injury and suppression of caspase-3 expression attenuated the hepatotoxicity induced cisplatin.

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“…Although the testes and epididymides weights 323 between the arsenic-exposed group and the control group were similar, the testes/body 324 weight ratio was decreased in male offspring of arsenic-treated mice both in F1 and 325 F3 generations. The Johnsen scoring system is widely used to evaluate 326 spermatogenesis in the seminiferous tubules(Naeimi et al 2017). In the current study, 327 reduced Johnsen scores and histopathological alterations were observed in the arsenic-328 exposed group in both F1 and F3 generations, which indicates poor spermatogenesis.329 Li et.…”

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confidence: 99%

Transgenerational inheritance of abnormal spermatogenesis with Igf2/H19 epigenetic alteration in CD1 mouse induced by in utero arsenic exposure

Yin¹,

Xia²,

Hou³

et al. 2020

Preprint

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Atorvastatin mitigates testicular injuries induced by ionizing radiation in mice

Cited by 53 publications

References 56 publications

Atorvastatin attenuates the ovarian damage induced by cyclophosphamide in rat: An experimental study

Atorvastatin attenuates the ovarian damage induced by cyclophosphamide in rat: An experimental study

Hydroalcoholic extract of Zataria multiflora mitigates cisplatin-induced oxidative stress, apoptosis and hepatotoxicity in mice

Transgenerational inheritance of abnormal spermatogenesis with Igf2/H19 epigenetic alteration in CD1 mouse induced by in utero arsenic exposure

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