Atopic dermatitis: immunophenotyping of inflammatory cells in skin lesions

Lugović, Liborija; Lipozenočić, Jasna; Jakić-Razumović, Jasminka

doi:10.1046/j.1365-4362.2001.01203.x

Cited by 29 publications

(33 citation statements)

References 22 publications

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“…Many authors report on the results of their long-term studies of various markers expressed on the surface of the cells infiltrating AD skin lesions [6, 13]. …”

Section: Discussionmentioning

confidence: 99%

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Prominent Involvement of Activated Th1-Subset of T-Cells and Increased Expression of Receptor for IFN-Gamma on Keratinocytes in Atopic Dermatitis Acute Skin Lesions

Lugović

Lipozenčić²,

Jakić-Razumović³

2005

Int Arch Allergy Immunol

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Background: Atopic dermatitis (AD) is an allergic skin disease mediated by antigen-specific IgE and an important role has been ascribed to CD4+ cells (Th cells). The objective of the study was to evaluate humoral and cellular immunological factors in the blood and the skin lesions of AD patients, and to analyze the presence of inflammatory cell-surface markers in blood and skin biopsies. Methods: The parameters for monitoring of 40 AD patients included results of prick test to inhalant allergens and epicutaneous (patch) test to contact allergens; values of total IgE, serum immunoglobulins (IgG, IgA, IgM) and different cell markers in the sera (CD3, CD4, CD8, CD20, CD21, CD23, HLA-DR). We also analyzed the presence of inflammatory cell-surface markers (CD3, CD4, CD8, CD20, CD20, CD1a, CD23, CD29, CD45Ro, IFNγ+ markers) in the biopsies of skin lesions from 10 AD patients and 5 healthy controls (HCs) by immunohistochemical analysis (method of avidin-biotin immunoperoxidase). Results: Beside increased total serum IgE and positive skin tests, a significantly higher percentage of CD23+ cells with lower percentage of CD21+ cells was revealed in peripheral blood of AD patients in comparison to HCs. A positive epidermal expression of the majority of markers of T cells (CD3+, CD4+, CD8+, CD29+, CD45Ro+, IFNγ+) and those of Langerhans’ cells (LCs) (CD1a, CD23+), without those of B cells (CD20+) were noted in AD patients, but no in the skin of HCs. Furthermore, significant difference was also found between the two groups for increased expression of CD3, CD4, CD8, CD29, CD45Ro, IFNγ+ markers (markers for IFNγ receptor) and higher intraepidermal CD23+ LCs and intradermal CD1a+ LCs in AD skin lesions. Conclusions:The obtained results suggest involvement of various humoral factors with increased production of IgE and cooperation between Th subsets and LCs, with higher production of related cytokines, and disturbed cellular immunity, including epidermal LCs with IgE receptors of high and low affinity in AD. The annotation of activated Th1 cells with increased producing of IFNγ in acute AD skin lesions is notable, and might lead to IFNγ binding to keratinocytes and consequently inflammatory skin changes in the disease.

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“…Many authors report on the results of their long-term studies of various markers expressed on the surface of the cells infiltrating AD skin lesions [6, 13]. …”

Section: Discussionmentioning

confidence: 99%

“…Many observations suggest that environmental allergens play an important role in the disease onset and exacerbation, which is demonstrated by in vivo (skin tests) and in vitro (e.g. determination of total and specific IgE antibodies) testing [5, 6]. …”

Section: Introductionmentioning

confidence: 99%

Prominent Involvement of Activated Th1-Subset of T-Cells and Increased Expression of Receptor for IFN-Gamma on Keratinocytes in Atopic Dermatitis Acute Skin Lesions

Lugović

Lipozenčić²,

Jakić-Razumović³

2005

Int Arch Allergy Immunol

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“…The epidermis, a stratified squamous epithelium, overlies the dermis, a mesenchymal tissue (Figure 1). In addition to fibroblasts and ECM components, the dermis, in common with other mesenchymal tissues, incorporates a wide range of other cell types, including blood vessel components (endothelial cells, smooth muscle cells, and blood vessel-associated fibroblasts) (7), eccrine and apocrine sweat glands, lymphatic vessel components, neurons (8), sensory receptors, and tissue-resident immune cells (9,10). These other cell types are sufficiently numerous that fibroblasts represent a minority cell population in the adult dermis.…”

Section: Introductionmentioning

confidence: 99%

Fibroblast heterogeneity: implications for human disease

Lynch

Watt

2018

Journal of Clinical Investigation

360

294

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“…Atopic dermatitis (AD) (eczema) is a chronically relapsing inflammatory skin disease associated with erythema, scaly and oozing plaques on the forehead and face, neck, hands and flexural areas and severe pruritus (1,2). AD affects 2-5% of the general population, with ≥10-20% occurrences in infants and children and 1-3% in adults (3).…”

Section: Introductionmentioning

confidence: 99%