“…In each case, the column eluent was directly coupled to an ICP-MS instrument for Hg quantication. Aer centrifugation and collection of the supernatant, 5 mL of a 0.1 mol L À1 acetate buffer at pH 5.0, and the 199 Hg and CH 3 201 Hg isotopically enriched spike materials suitable to obtain a spike : reference isotope amount ratio of close to unity, were added to 0.1 mL of the CRM extract, the pH was readjusted to 5.0 followed by the addition of 1 mL of hexane and 1 mL of either 1% NaBPr 4 or 1% NaBEt 4 However, as the authors state, both methods exhibited overall good performance for the analysis of the CRM. Four mercury species, Hg 2+ , MeHg, EtHg and PhHg, were separated in this order in under ve minutes using these conditions.…”