Atomic Hydrogen Surrounded by Water Molecules, H(H2O)m, Modulates Basal and UV-Induced Gene Expressions in Human Skin In Vivo

Shin, Mi Hee; Park, Raeeun; Nojima, Hideo; Kim, Hyung Chel; Kim, Yeon Kyung; Chung, Jin Ho

doi:10.1371/journal.pone.0061696

Cited by 22 publications

(18 citation statements)

References 55 publications

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“…4a). UV-B irradiation also activates NF-jB, a transcription factor that activates multiple genes involved in the expression of various proinflammatory mediators (51). The present study has shown that GA treatment inhibited the translocation of NF-jB from cytosol to the nucleus and reduced its protein content in UV-B-irradiated HaCaT cells and BALB/c mouse skin (Figs 2b, S3d, S6a, S6c).…”

Section: Discussionsupporting

confidence: 57%

Glycyrrhizic acid prevents ultraviolet‐B‐induced photodamage: a role for mitogen‐activated protein kinases, nuclear factor kappa B and mitochondrial apoptotic pathway

Afnan

Kaiser

Rafiq

et al. 2016

Experimental Dermatology

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Glycyrrhizic acid (GA), a natural triterpene, has received attention as an agent that has protective effects against chronic diseases including ultraviolet UV-B-induced skin photodamage. However, the mechanism of its protective effect remains elusive. Here, we used an immortalized human keratinocyte cell line (HaCaT) and a small animal model (BALB/c mice), to investigate the protective effects of GA against UV-B-induced oxidative damage, and additionally, delineated the molecular mechanisms involved in the UV-B-mediated inflammatory and apoptotic response. In the HaCaT cells, GA inhibited the UV-B-mediated increase in intracellular reactive oxygen species (ROS) and down-regulated the release of pro-inflammatory cytokines interleukin (IL)-1α, -1β and -6, tumor necrosis factor (TNF)-α and prostaglandin E2 (PGE2). GA inhibited UV-B-mediated activation of p38 and JNK MAP kinases, COX-2 expression and nuclear translocation of NF-κB. Furthermore, GA inhibited UV-B-mediated apoptosis by attenuating translocation of Bax from the cytosol to mitochondria, thus preserving mitochondrial integrity. GA-treated HaCaT cells also exhibited elevated antiapoptotic Bcl-2 protein, concomitant with reduced caspase-3 cleavage and decreased PARP-1 protein. In BALB/c mice, topical application of GA on dorsal skin exposed to UV-B irradiation protected against epidermal hyperplasia, lymphocyte infiltration and expression of several inflammatory proteins, p38, JNK, COX-2, NF-κB and ICAM-1. Based on the above findings, we conclude that GA protects against UV-B-mediated photodamage by inhibiting the signalling cascades triggered by oxidative stress, including MAPK/NF-κB activation, as well as apoptosis. Thus, GA has strong potential to be used as a therapeutic/cosmeceutical agent against photodamage.

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Section: Discussionsupporting

confidence: 57%

Glycyrrhizic acid prevents ultraviolet‐B‐induced photodamage: a role for mitogen‐activated protein kinases, nuclear factor kappa B and mitochondrial apoptotic pathway

Afnan

Kaiser

Rafiq

et al. 2016

Experimental Dermatology

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“…Interestingly, in a recent study by Mi Hee Shin et al62, they observed that H(H2O) (atomic hydrogen surrounded by water molecules) application to human skin prevented UV-induced erythema and DNA damage. In their stuy, H(H2O) significantly prevented UV-induced MMP-1, COX-2, IL-6 and IL-1β mRNA expressions in human skin in vivo.…”

Section: Skinmentioning

confidence: 99%

Hydrogen as a New Class of Radioprotective Agent

Qian¹,

Shen²,

Chuai³

et al. 2013

Int. J. Biol. Sci.

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It is well known that most of the ionizing radiation-induced damage is caused by hydroxyl radicals (·OH) follows radiolysis of H2O. Molecular hydrogen (H2) has antioxidant activities by selectively reducing ·OH and peroxynitrite(ONOO-). We firstly hypothesized and demonstrated the radioprotective effect of H2 in vitro and in vivo, which was also repeated on different experimental animal models by different departments. A randomized, placebo-controlled study showed that consumption of hydrogen-rich water reduces the biological reaction to radiation-induced oxidative stress without compromising anti-tumor effects. These encouraging results suggested that H2 represents a potentially novel preventative strategy for radiation-induced oxidative injuries. H2 is explosive. Therefore, administration of hydrogen-rich solution (physiological saline/pure water/other solutions saturated with H2) may be more practical in daily life and more suitable for daily consumption. This review focuses on major scientific and clinical advances of hydrogen-rich solution/H2 as a new class of radioprotective agent.

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“…In particular, TGF-β1 induces the production of type I procollagen (Lee et al, 2012). Interleukin (IL)-6, a cytokine produced by various cells, such as fibroblasts, is a signal molecule that mediates the inflammatory response (Shin et al, 2013;Wu et al, 2013) and is also known to be associated with ROS caused by UV radiation. Inflammation increases production of ROS and cytokines, accelerating the aging process (Thiex and Richardson, 2003;Pillai et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Gallic Acid Regulates Skin Photoaging in UVB‐exposed Fibroblast and Hairless Mice

Hwang

Park

Lee

et al. 2014

Phytotherapy Research

127

102

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Ultraviolet (UV) radiation is the primary factor in skin photoaging, which is characterized by wrinkle formation, dryness, and thickening. The mechanisms underlying skin photoaging are closely associated with degradation of collagen via upregulation of matrix metalloproteinase (MMP) activity, which is induced by reactive oxygen species (ROS) production. Gallic acid (GA), a phenolic compound, possesses a variety of biological activities including antioxidant and antiinflammatory activities. We investigated the protective effects of GA against photoaging caused by UVB irradiation using normal human dermal fibroblasts (NHDFs) in vitro and hairless mice in vivo. The production levels of ROS, interlukin-6, and MMP-1 were significantly suppressed, and type I procollagen expression was stimulated in UVB-irradiated and GA-treated NHDFs. GA treatment inhibited the activity of transcription factor activation protein 1. The effects of GA following topical application and dietary administration were examined by measuring wrinkle formation, histological modification, protein expression, and physiological changes such as stratum corneum hydration, transepidermal water loss, and erythema index. We found that GA decreased dryness, skin thickness, and wrinkle formation via negative modulation of MMP-1 secretion and positive regulation of elastin, type I procollagen, and transforming growth factor-β1. Our data indicate that GA is a potential candidate for the prevention of UVB-induced premature skin aging.

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Atomic Hydrogen Surrounded by Water Molecules, H(H2O)m, Modulates Basal and UV-Induced Gene Expressions in Human Skin In Vivo

Cited by 22 publications

References 55 publications

Glycyrrhizic acid prevents ultraviolet‐B‐induced photodamage: a role for mitogen‐activated protein kinases, nuclear factor kappa B and mitochondrial apoptotic pathway

Glycyrrhizic acid prevents ultraviolet‐B‐induced photodamage: a role for mitogen‐activated protein kinases, nuclear factor kappa B and mitochondrial apoptotic pathway

Hydrogen as a New Class of Radioprotective Agent

Gallic Acid Regulates Skin Photoaging in UVB‐exposed Fibroblast and Hairless Mice

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