Atomic force spectroscopy-based study of antibody pesticide interactions for characterization of immunosensor surface

Kaur, Jasdeep; Singh, Kanwar Vikas; Schmid, Ashwini Hirlekar; Varshney, Grish C.; Suri, C. Raman; Raje, Manoj

doi:10.1016/j.bios.2004.01.012

Cited by 52 publications

(37 citation statements)

References 37 publications

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“…By measuring an in situ membrane protein complex stabilized by the membrane bilayer and by interactions with neighbors, repeated measurements are possible that report functional interactions, with no extraction or unfolding of the cytb 6 f complex. Such stabilizing influences allow measurement of the ;300 pN unbinding forces, which are comparable with those obtained using conventional force spectroscopy for a range of other interactions including 340 to 454 pN for streptavidin-biotin (Lee et al, 1994;Stevens et al, 2002), 507 pN for the hCG-anti-hCG pair (Stevens et al, 2002), 512 pN for the MPAD-anti-MPAD interaction (Kaur et al, 2004), and 420 pN for citrate synthase-GroEL (Vinckier et al, 1998). The forces we measure likely sample the binding interaction prior to electron transfer, given the requirement for both reduced cytb 6 f and oxidized Pc, as well as the similarity of the dwell time (;160 ms at 1.0 kHz force curve repetition rate) of the AFM probe on the membrane surface to the ;70 to 130 ms electron transfer between Pc and cytb 6 f (Haehnel et al, 1980;Delosme, 1991).…”

Section: Affinity-mapping Afm Using Pc-functionalized Afm Probes Allomentioning

confidence: 60%

Nanodomains of Cytochromeb 6 fand Photosystem II Complexes in Spinach Grana Thylakoid Membranes

et al. 2014

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The cytochrome b 6 f (cytb 6 f) complex plays a central role in photosynthesis, coupling electron transport between photosystem II (PSII) and photosystem I to the generation of a transmembrane proton gradient used for the biosynthesis of ATP. Photosynthesis relies on rapid shuttling of electrons by plastoquinone (PQ) molecules between PSII and cytb 6 f complexes in the lipid phase of the thylakoid membrane. Thus, the relative membrane location of these complexes is crucial, yet remains unknown. Here, we exploit the selective binding of the electron transfer protein plastocyanin (Pc) to the lumenal membrane surface of the cytb 6 f complex using a Pc-functionalized atomic force microscope (AFM) probe to identify the position of cytb 6 f complexes in grana thylakoid membranes from spinach (Spinacia oleracea). This affinity-mapping AFM method directly correlates membrane surface topography with Pc-cytb 6 f interactions, allowing us to construct a map of the grana thylakoid membrane that reveals nanodomains of colocalized PSII and cytb6f complexes. We suggest that the close proximity between PSII and cytb 6 f complexes integrates solar energy conversion and electron transfer by fostering short-range diffusion of PQ in the protein-crowded thylakoid membrane, thereby optimizing photosynthetic efficiency.

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Section: Affinity-mapping Afm Using Pc-functionalized Afm Probes Allomentioning

confidence: 60%

Nanodomains of Cytochromeb 6 fand Photosystem II Complexes in Spinach Grana Thylakoid Membranes

et al. 2014

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“…800-1200 pN) (Lee et al, 1994) or immunochemical complex (ca. 60-250 pN) (Kaur et al, 2004), but greater than that required to remove beads bound to the surface by weak, nonspecific interactions (ca. 0.1-10 pN).…”

Section: Resultsmentioning

confidence: 99%

Three-Dimensional Near Infrared Imaging of Pathophysiological Changes Within the Breast

Yalavarthy¹

2008

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A significant challenge for all biosensor systems is to achieve high assay sensitivity and specificity while minimizing sample preparation requirements, operational complexity, and sample-to-answer time. We have achieved multiplexed, unamplified, femtomolar detection of both DNA and proteins in complex matrices (including whole blood, serum, plasma, and milk) in minutes using as few as two reagents by labeling conventional assay schemes with micrometerscale magnetic beads, and applying fluidic force discrimination (FFD). In FFD assays, analytes captured onto a microarray surface are labeled with microbeads, and a controlled laminar flow is then used to apply microfluidic forces sufficient to preferentially remove only nonspecifically bound bead labels. The density of beads that remain bound is proportional to the analyte concentration and can be determined with either optical counting or magnetoelectronic detection of the magnetic labels. Combining FFD assays with chip-based magnetoelectronic detection enables a simple, potentially handheld, platform capable of both nucleic acid hybridization assays and immunoassays, including orthogonal detection and identification of bacterial and viral pathogens, and therefore suitable for a wide range of biosensing applications. Report Documentation Page Form Approved OMB No. 0704-0188Public reporting burden for the collection of information is estimated to average 1 hour per response, including the time for reviewing instructions, searching existing data sources, gathering and maintaining the data needed, and completing and reviewing the collection of information. Send comments regarding this burden estimate or any other aspect of this collection of information, including suggestions for reducing this burden, to Washington Headquarters Services, Directorate for Information Operations and Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington VA 22202-4302. Respondents should be aware that notwithstanding any other provision of law, no person shall be subject to a penalty for failing to comply with a collection of information if it does not display a currently valid OMB control number. A significant challenge for all biosensor systems is to achieve high assay sensitivity and specificity while minimizing sample preparation requirements, operational complexity, and sample-to-answer time. We have achieved multiplexed, unamplified, femtomolar detection of both DNA and proteins in complex matrices (including whole blood, serum, plasma, and milk) in minutes using as few as two reagents by labeling conventional assay schemes with micrometerscale magnetic beads, and applying fluidic force discrimination (FFD). In FFD assays, analytes captured onto a microarray surface are labeled with microbeads, and a controlled laminar flow is then used to apply microfluidic forces sufficient to preferentially remove only nonspecifically bound bead labels. The density of beads that remain bound is proportional to the analyte concentration and can be determined with either optical co...

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“…Kaur et al (2004) created oriented active immunobiosensor surface by immobilization of antibodies on the gold surface against two herbicide molecules 2,4-dichlorophenoxyacetic acid and atrazine. Analysis of the adhesive forces, between immobilized antibodies and their respective antigens by force spectroscopy using hapten-carrier protein functionalized atomic force microscope (AFM) cantilevers, showed that immobilization had not compromised the reactivity of the surface immobilized antibody molecules for antigen and there was no change in their relative quality with respect to each other.…”

Section: Detection Of Pesticide Residuesmentioning

confidence: 99%

Optical biosensors for food quality and safety assurance—a review

et al. 2011

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Food quality and safety is a scientific discipline describing handling, preparation and storage of food in ways that prevent food borne illness. Food serves as a growth medium for microorganisms that can be pathogenic or cause food spoilage. Therefore, it is imperative to have stringent laws and standards for the preparation, packaging and transportation of food. The conventional methods for detection of food contamination based on culturing, colony counting, chromatography and immunoassay are tedious and time consuming while biosensors have overcome some of these disadvantages. There is growing interest in biosensors due to high specificity, convenience and quick response. Optical biosensors show greater potential for the detection of pathogens, pesticide and drug residues, hygiene monitoring, heavy metals and other toxic substances in the food to check whether it is safe for consumption or not. This review focuses on optical biosensors, the recent developments in the associated instrumentation with emphasis on fiber optic and surface plasmon resonance (SPR) based biosensors for detecting a range of analytes in food samples, the major advantages and challenges associated with optical biosensors. It also briefly covers the different methods employed for the immobilization of bio-molecules used in developing biosensors.

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Atomic force spectroscopy-based study of antibody pesticide interactions for characterization of immunosensor surface

Cited by 52 publications

References 37 publications

Nanodomains of Cytochromeb 6 fand Photosystem II Complexes in Spinach Grana Thylakoid Membranes

Nanodomains of Cytochromeb 6 fand Photosystem II Complexes in Spinach Grana Thylakoid Membranes

Three-Dimensional Near Infrared Imaging of Pathophysiological Changes Within the Breast

Optical biosensors for food quality and safety assurance—a review

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